Oriented immobilization of galactose oxidase to bead and magnetic bead cellulose and poly(HEMA-co-EDMA) and magnetic poly(HEMA-co-EDMA) microspheres

Effects of organic solvents on the activity of free and immobilised laccase from Rhus vernicifera

“…There are various reports on the effects of the carbohydrate part on enzyme activity [36], however, binding of support molecules too close to the active centres can result in a decrease in, or complete loss of, enzyme activity [37]. The activity of several enzymes remained mostly intact when the carbohydrate part of the glycoprotein was oxidised using sodium periodate under mild conditions [38], and therefore this immobilisation methodology was selected for use.…”

Carbon dioxide/methanol conversion cycle based on cascade enzymatic reactions supported on superparamagnetic nanoparticles

“…For instance, coupling reactions can be performed using reactive groups such as amines, carboxylates and thiols. Carboxylic groups can be activated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, or EDC (Kondo and Fukuda 1997) in order to bind amines, yielding stable amide bonds linking the enzyme to the support (Bílková et al 2002, Hong et al 2007, Demirel et al 2004, Demirel and Mutlu 2005, Zeng et al 2006, Guo et al 2003, Iman et al 1992, Pan et al 2009). Amine groups can be linked by means of glutaraldehyde, forming imine bonds, which can further be reduced with NaBH 4 .…”

Preparation of magnetic strong cation exchange resin for the extraction of melamine from egg samples followed by liquid chromatography–tandem mass spectrometry

“…In the classical sample preparation techniques, food samples are usually extracted with solvent under shaking [14], vortexing [15] or ultrasonic [3,4], followed by centrifugation, and then the extract obtained is cleaned-up by liquid-liquid extraction [2] or solid phase extraction (SPE) [14,19,22], which results in relatively complex sample preparation procedure, lower extraction yield, and re-extraction is often required [3]. Magnetic separation technology has received considerable attention in recent two decades due to the rapid and easy sample preparation procedure it provides [23] and the great potential for applications in cell separation [24,25], drug delivery [26,27] and enzyme immobilization [28] it has. Generally, magnetic adsorbent is prepared by encapsulating inorganic magnetic particle with organic monomer [29].…”