Oriented collagen tubes combined with basic fibroblast growth factor promote peripheral nerve regeneration in a 15 mm sciatic nerve defect rat model

Fujimaki, Hisako; Uchida, Katsuhisa; Inoue, Gen; Miyagi, Masayuki; Nemoto, Noriko; Saku, Taro; Isobe, Yoshihiro; Inage, Kazuhide; Matsushita, Osamu; Yagishita, Saburo; Sato, Jun; Takano, Shotaro; Sakuma, Yoshihiro; Ohtori, Seiji; Takahashi, Kazuhisa; Takaso, Masashi

doi:10.1002/jbm.a.35866

Cited by 48 publications

(43 citation statements)

References 21 publications

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“…bFGF administration promotes Schwann cell growth and nerve regeneration in areas of peripheral nerve injury . While bFGF has high therapeutic potential, its efficiency is quickly reduced after it diffuses from the area of injury into the extracellular fluid .…”

Section: Discussionmentioning

confidence: 99%

Wrapping With Basic Fibroblast Growth Factor‐Impregnated Collagen Sheet Reduces Rat Sciatic Nerve Allodynia

Mukai

Uchida

Hirosawa

et al. 2019

Journal Orthopaedic Research

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Autologous vein wrapping is used to treat recurrent chronic constriction neuropathy and traumatic peripheral nerve injury. However, its use is restricted due to the inability to obtain sufficiently long veins for larger grafts. We previously reported that vein‐derived basic fibroblast growth factor (bFGF) promotes heme oxygenase‐1 (HO‐1), which reduces allodynia via its anti‐inflammatory properties. To mimic vein wrapping, we developed a collagen sheet impregnated with bFGF. Chronic constriction injury (CCI) was induced in male Wistar rats as a model of sciatic nerve injury, and the rats were divided into three groups: (i) untreated after CCI surgery (control group), (ii) treated with a collagen sheet wrap impregnated with phosphate‐buffered saline (PBS/CS group), and (iii) treated with a collagen sheet wrap impregnated with bFGF (bFGF/CS group). Pain behavior (von Frey test) was evaluated on postoperative days (PODs) 1, 5, 7, and 14. Quantitative polymerase chain reaction was conducted on sciatic nerve RNA to quantify HO‐1 gene, Hmox1, expression. Enzyme‐linked immunosorbent assay were used to determine HO‐1 protein levels on POD 1. von Frey testing showed significantly greater pain hypersensitivity in the control and PBS/CS groups than the bFGF/CS group. In the bFGF/CS group, Hmox1 messenger RNA and HO‐1 protein levels were significantly increased in the sciatic nerve compared with the control and PBS/CS groups on PODs 1 and 5 and POD 1, respectively. The bFGF/CS group showed decreased allodynia and HO‐1 induction, as observed with vein wrapping. Therefore, local application of bFGF may be an alternative treatment strategy for compressive neuropathy and peripheral nerve trauma in clinical settings. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2258–2263, 2019

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Section: Discussionmentioning

confidence: 99%

Wrapping With Basic Fibroblast Growth Factor‐Impregnated Collagen Sheet Reduces Rat Sciatic Nerve Allodynia

Mukai

Uchida

Hirosawa

et al. 2019

Journal Orthopaedic Research

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“…Here, bFGF‐positive cells were observed in the endothelial surface and bFGF mRNA expression in veins was fivefold higher than that in the sciatic nerve. Several recent studies demonstrated that the application of exogenous bFGF with a carrier‐mediated controlled release system facilitated nerve regeneration in transected rat sciatic nerves . For example, Fujimoto et al used electron microscopy to examine the in vivo bFGF treatment of rat sciatic nerves and found that regenerating axons grew 1.5–2‐fold faster when nerve stumps were treated with bFGF compared with phosphate‐buffered saline.…”

Section: Discussionmentioning

confidence: 99%

“…During the early phase of peripheral nerve injury, the heparin‐binding protein basic fibroblast growth factor (bFGF) has neuroprotective effects and contributes to the proliferation of undifferentiated Schwann cells and nerve regeneration . Venous endothelial and smooth muscle cells contain large quantities of bFGF .…”

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confidence: 99%

Vein wrapping facilitates basic fibroblast growth factor‐induced heme oxygenase‐1 expression following chronic nerve constriction injury

Hirosawa

Uchida

Kuniyoshi

et al. 2017

Journal Orthopaedic Research

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The clinical efficacy of autologous vein wrapping for recurrent compressive neuropathy has been demonstrated; however, the underlying mechanisms of this technique remain unclear. Rats were divided into chronic constriction injury (CCI) and CCI + vein wrapping (CCI + VW) groups. Mechanical allodynia was evaluated using von Frey filaments. To identify the neuroprotective factors released from veins, basic fibroblast growth factor (bFGF) mRNA expression in veins was compared to that in the sciatic nerve. The response of heme oxygenase-1 (HO-1) expression to vein wrapping was evaluated by RT-PCR and enzyme-linked immunosorbent assays. The effects of exogenous bFGF on HO-1 expression were evaluated using a sciatic nerve cell culture. Vein wrapping significantly increased the withdraw threshold levels compared to the untreated CCI group. bFGF mRNA expression in veins was higher than that in untreated sciatic nerves. HO-1 mRNA expression was induced at higher levels in sciatic nerve cells in the presence of exogenous bFGF compared to untreated control cells. HO-1 mRNA and protein expression in the sciatic nerve were also higher in the CCI + VW group compared with the CCI group. Our results suggest that vein-derived bFGF contributes to the therapeutic benefit of vein wrapping through the induction of HO-1 in the sciatic nerve. Vein wrapping is a useful technique for reducing neuropathic pain. Further understanding of the neurotrophic factors released from veins may help to optimize current procedures for treating recurrent compressive neuropathy and traumatic peripheral nerve injury, and lead to the development of new therapeutic methods using recombinant neurotrophic factors. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:898-905, 2018.

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“…Researchers often choose from wide variety of gait parameters to report their findings without justification of selection preference (Freria et al, 2016; Fujimaki et al, 2016; Hausner et al, 2014; Huang et al, 2012), and there are conflicting interpretations of results across different injury models (Bozkurt et al, 2008; Hamers et al, 2006). The lack of a standard set of parameters and interpretations may due to common but seldom mentioned problems following rat sciatic nerve injury such as preferential heel walking (Deumens et al, 2007; Deumens et al, 2014) and poor compliance (Neckel, 2015).…”

Section: Introductionmentioning

confidence: 99%

Establishing a reliable gait evaluation method for rodent studies

Chen

Zhang

et al. 2017

Journal of Neuroscience Methods

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BACKGROUND CatWalk is one of the most popular tools for evaluating gait recovery in preclinical research, however, there is currently no consensus on which of the many gait parameters captured by CatWalk can reliably model recovery. There are conflicting interpretations of results, along with many common but seldom reported problems such as heel walking and poor compliance. NEW METHOD We developed a systematic manual classification method that overcomes common problems such as heel walking and poor compliance. By correcting automation errors and removing inconsistent gait cycles, we isolated stretches of recordings that are more reliable for analysis. Recovery outcome was also assessed by quantitative histomorphometric analysis of myelinated axons. RESULTS While 40–60% of runs were erroneously classified without manual intervention, we corrected all errors with our new method, and showed that Stand Time, Duty Cycle, and Swing Speed are able to track significant differences over time and between experimental groups (all p<0.05). The usability of print area and intensity parameters requires further validation beyond the capabilities of CatWalk. COMPARISON WITH EXISTING METHOD(S) There is currently no strategy that addresses problems such as heel walking and poor compliance, and therefore no standard set of parameters that researchers can rely on to report their findings. CONCLUSION Manual classification is a crucial step to generate reliable CatWalk data, and Stand Time, Duty Cycle, and Swing Speed are suitable parameters for evaluating gait recovery. Static parameters such as print area and intensity should be used with extreme caution.

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Oriented collagen tubes combined with basic fibroblast growth factor promote peripheral nerve regeneration in a 15 mm sciatic nerve defect rat model

Cited by 48 publications

References 21 publications

Wrapping With Basic Fibroblast Growth Factor‐Impregnated Collagen Sheet Reduces Rat Sciatic Nerve Allodynia

Wrapping With Basic Fibroblast Growth Factor‐Impregnated Collagen Sheet Reduces Rat Sciatic Nerve Allodynia

Vein wrapping facilitates basic fibroblast growth factor‐induced heme oxygenase‐1 expression following chronic nerve constriction injury

Establishing a reliable gait evaluation method for rodent studies

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