Orientations of the<i>Bacteroides fragilis</i>Capsular Polysaccharide Biosynthesis Locus Promoters during Symbiosis and Infection

Troy, Erin B.; Carey, Vincent J.; Kasper, Dennis L.; Comstock, Laurie E.

doi:10.1128/jb.00555-10

Cited by 21 publications

(15 citation statements)

References 20 publications

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“…Surface polysaccharide expression and phase variation is important for B. fragilis colonization 28 . However, the kinetics of carbohydrate shedding, turnover, and production are unknown.…”

Section: Resultsmentioning

confidence: 99%

In vivo imaging and tracking of host–microbiota interactions via metabolic labeling of gut anaerobic bacteria

Geva‐Zatorsky

Álvarez

Hudak

et al. 2015

Nat Med

201

197

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The intestine is densely populated by anaerobic commensal bacteria. These microorganisms shape immune system development, but our understanding of host–commensal interactions is hampered by a lack of tools for studying the anaerobic intestinal environment. We applied metabolic oligosaccharide engineering and bioorthogonal click-chemistry to label various commensal anaerobes, including Bacteroides fragilis, a common and immunologically important commensal. We studied the dissemination of B. fragilis following acute peritonitis, and characterized the interactions of the intact microbe and its polysaccharide components in myeloid and B cell lineages. The distribution and colonization of labeled B. fragilis along the intestine can be assessed, as well as niche competition following coadministration of multiple species of the microbiota. Nine additional anaerobic commensals (both gram-negative and gram-positive) from three phyla common in the gut—Bacteroidetes, Firmicutes, and Proteobacteria—and five families and one aerobic pathogen (Staphylococcus aureus) were also fluorescently labeled. This strategy permits visualization of the anaerobic microbial niche by various methods, including intravital two-photon microscopy and non-invasive whole-body imaging, and an approach to study microbial colonization and host–microbe interactions in real-time.

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“…Surface polysaccharide expression and phase variation is important for B. fragilis colonization 28 . However, the kinetics of carbohydrate shedding, turnover, and production are unknown.…”

Section: Resultsmentioning

confidence: 99%

In vivo imaging and tracking of host–microbiota interactions via metabolic labeling of gut anaerobic bacteria

Geva‐Zatorsky

Álvarez

Hudak

et al. 2015

Nat Med

201

197

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“…The frequency of phase variants under conditions of laboratory culture is known to differ from that seen under conditions of growth in an animal host ( 20 , 21 ). To determine the frequency of this phase variant in vivo , we next analyzed whole-genome-shotgun (WGS) metagenomic sequence data from Human Microbiome Project (HMP) stool samples in an effort to find a subject colonized predominantly by B. thetaiotaomicron .…”

Section: Resultsmentioning

confidence: 99%

A Phase-Variable Surface Layer from the Gut Symbiont Bacteroides thetaiotaomicron

Taketani

Donia

Jacobson

et al. 2015

mBio

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The capsule from Bacteroides, a common gut symbiont, has long been a model system for studying the molecular mechanisms of host-symbiont interactions. The Bacteroides capsule is thought to consist of an array of phase-variable polysaccharides that give rise to subpopulations with distinct cell surface structures. Here, we report the serendipitous discovery of a previously unknown surface structure in Bacteroides thetaiotaomicron: a surface layer composed of a protein of unknown function, BT1927. BT1927, which is expressed in a phase-variable manner by ~1:1,000 cells in a wild-type culture, forms a hexagonally tessellated surface layer. The BT1927-expressing subpopulation is profoundly resistant to complement-mediated killing, due in part to the BT1927-mediated blockade of C3b deposition. Our results show that the Bacteroides surface structure is capable of a far greater degree of structural variation than previously known, and they suggest that structural variation within a Bacteroides species is important for productive gut colonization.

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“…7 Although the factors driving the promoter orientations of B. fragilis in the gut are incompletely understood, the environmental factors, such as the presence of complex microbiota, influence the promoter orientations. 7 Capsular PS enhance the stability of the bacterium at the mucosal niche, 6 and also elicit host immune responses, which may in turn influence bacterial colonization. [9][10][11] Therefore, PS promoter orientations may be important for the adaptation of bacteria -commensals and potential pathogens -in the gut environment.…”

Section: Bacterial Adaptation In the Steady-state Gutmentioning

confidence: 99%

Microbial adaptation to the healthy and inflamed gut environments

2020

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Orientations of theBacteroides fragilisCapsular Polysaccharide Biosynthesis Locus Promoters during Symbiosis and Infection

Cited by 21 publications

References 20 publications

In vivo imaging and tracking of host–microbiota interactions via metabolic labeling of gut anaerobic bacteria

In vivo imaging and tracking of host–microbiota interactions via metabolic labeling of gut anaerobic bacteria

A Phase-Variable Surface Layer from the Gut Symbiont Bacteroides thetaiotaomicron

Microbial adaptation to the healthy and inflamed gut environments

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