Orientational relaxation times of rhodamine 700 in glycerol-water mixtures

Megens, Mischa; Sprik, R.; Wegdam, G.H.; Lagendijk, Ad

doi:10.1063/1.474410

Cited by 21 publications

(17 citation statements)

References 19 publications

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“…The dependence of lifetime on viscosity can be evaluated by determining the parameter α in eq from the slope of a log–log plot of Figure b, which is 0.28 for the viscosity-dependent region (from 0.94 to 14.6 cP) and virtually zero above 41.1 cP. Interestingly, similar observations have been made for the orientational correlation times of different dyes in water–glycerol mixtures . This is explained as a change in the boundary conditions from a stick to a slip boundary at high viscosities, which leads to the formation of cavities. , At high viscosities, the cavity achieves a limiting rigidity that does not change with further changes in viscosities, and thus, the lifetime becomes independent of viscosity.…”

Section: Resultsmentioning

confidence: 57%

Sensing Temperature in Vitro and in Cells Using a BODIPY Molecular Probe

Ogle¹,

McWilliams²,

Ware

et al. 2019

J. Phys. Chem. B

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Boron dipyrromethene (BODIPY) molecular rotors have shown sensitivity toward viscosity, polarity, and temperature. Here, we report a 1,3,5,7-tetramethyl-8-phenyl-BODIPY modified with a polyethylene glycol (PEG) chain, for temperature sensing and live cell imaging. This new PEG-BODIPY dye presents an increase in nonradiative decay as temperature increases, which directly influences its lifetime. This change in lifetime is dependent on changes in both temperature and viscosity at low viscosity values, but is only dependent on temperature at high viscosity values. The dependence of fluorescence lifetime with temperature allows for temperature monitoring in vitro and in cells, with sub degree resolution. When in contact with cells, the PEG-BODIPY spontaneously penetrates and stains the cell but not the nucleus. Furthermore, no significant cell toxicity was found even at 100 μM concentration. Using fluorescence lifetime imaging microscopy (FLIM), we were able to observe the changes in the lifetime of PEG-BODIPY within the cell at different temperatures. The use of FLIM and molecular probes such as PEG-BODIPY can provide important information about cellular temperature and heat dissipation upon medically relevant stimuli, such as radiofrequency ablation and photodynamic therapy.

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Section: Resultsmentioning

confidence: 57%

Sensing Temperature in Vitro and in Cells Using a BODIPY Molecular Probe

Ogle¹,

McWilliams²,

Ware

et al. 2019

J. Phys. Chem. B

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“…Furthermore, in one time-resolved study conducted in our laboratory with rhodamine 101 in an acidified ethanol-water mixture, 17 we also obtained a limiting anisotropy much closer to 0.4 than those previously published. 9 On the other hand, early 18,19 and more recent [20][21][22] time-resolved determinations of the limiting anisotropies of several xanthene dyes, including rhodamine 101, yielded values in the range 0.37-0.40 with a typical precision of 0.02, insufficient to decide between the two alternatives. All these observations prompted us to study in more detail the fluorescence polarization of rhodamine 101, and to determine its limiting value with unprecedented accuracy, quantifying at the same time the effect of some factors that may explain the substantially lower values reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

Accurate Determination of the Limiting Anisotropy of Rhodamine 101. Implications for Its Use as a Fluorescence Polarization Standard

et al. 2008

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The S 1 -S 0 limiting anisotropy of a widely used fluorophore, rhodamine 101, is determined with unprecedented accuracy. From time-resolved and steady-state fluorescence measurements in several solvents, it is shown that the limiting anisotropy of rhodamine 101 is for all practical purposes equal to the theoretical one-photon fundamental anisotropy value of 2/5, both in rigid and in fluid media. This fact, along with the favorable chemical and photophysical properties of rhodamine 101, point to its use as a standard for fluorescence polarization measurements. It is also shown that if the excitation pulse can be considered a delta impulse with respect to the time scale of the anisotropy decay (but not necessarily to the time scale of the intensity decay), then no deconvolution procedure is needed for anisotropy decay analysis. IntroductionRhodamine 101 (also known as rhodamine 640), Chart 1, and derivatives retaining the same fluorophore are widely used as fluorescent probes. Rhodamine 101 was designed so that no rotation of the amino groups is possible, 1,2 in the hope of achieving unit fluorescence quantum yield, but this was not fulfilled. 3 Indeed, its fluorescence quantum yield, although high, is similar to that of rhodamine 6G [3][4][5] and is in fact distinctly lower than 1.0, 4-6 which is the value initially reported. 1,2,7 In room temperature acidified ethanol, where the dye is present in the cationic form, it has a fluorescence quantum yield of about 0.9 6 and a single exponential fluorescence decay with a lifetime of 4.3 ns. 6 The cation and the zwitterion share a common fluorophore, whereas the lactone form has quite different photophysical properties. 8 A precise determination of the S 1 -S 0 limiting anisotropy (i.e., the highest measured S 1 -S 0 anisotropy for a given molecule) of several xanthene dyes, including the rhodamines B, 6G and 101, and fluorescein, r 0 ) 0.373 ( 0.002, was reported by Johansson, 9 on the basis of both steady-state and time-resolved measurements. This begs the question as to why the limiting anisotropy is lower than the fundamental anisotropy, whose onephoton theoretical value is 0.4 for parallel absorption and emission transition moments.The fact that the limiting anisotropy seldom attains the theoretical value of 2 / 5 has been subject of considerable attention over the years; see, e.g., the classic work of Feofilov 10 for an early view, and Valeur's book 11 for a recent discussion. Possible reasons for the discrepancy fall in four categories: (i) instrumental effects (effect of wide angle collection and/or of polarizer misalignment, 12,13 etc.); (ii) matrix-dependent effects (depolarization by light scattering, depolarization by stress-induced optical activity in solid glasses, depolarization by residual rotational motion); (iii) intermolecular effects (depolarization by radiative and/or nonradiative energy migration); (iv) intramolecular effects (mixed polarization bands, significantly different geometries for the Franck-Condon and emissive states implying noncoinci...

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“…It is reported in the literature that an increase in solvent viscosity increases both the probe fluorescence lifetime [16,17] and the rotational relaxation time [18], which is the case for MEG relative to water. Concentrations between 1.8 × 10 −3 and 2.2 × 10 −3 mol L −1 were chosen in order to satisfy all these constraints on the fluorescent probe and obtain reliable signal intensity.…”

Section: Methodsmentioning

confidence: 97%

Detection of chemical alterations at internal walls of microchannel flow cells by nondestructive fluorescence depolarization

Quintella¹,

Lima²,

Mammana³

et al. 2004

Journal of Colloid and Interface Science

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A recent trend is the production of workable microchannel flow cells (MF cells). The nondestructive methods used to assess their reliability are based mainly on output monitoring and do not evaluate internal chemical interactions. We investigate a nondestructive method for evaluating changes in the chemical composition of the inner walls based on evaluation of the extent of alignment of a fluorescent probe in a liquid flowing within MF cells. Two MF cells were built with a 10-microm inner spacing. Their inner walls had four parallel SnO(2) strips, 2.00 mm wide, separated by 0.50-mm-wide glass strips. One cell had strips parallel to the flow and the other perpendicular. Flow-induced intermolecular alignment of rhodamine B in monoethylene glycol was scanned with 28-microm precision by fluorescence depolarization, using polarized-laser-induced fluorescence within induced flows (PLF-FI). No changes of polarization were seen when the flow was stopped. Under flowing conditions, polarization was always 4% lower in the glass region as compared to SnO(2). Glass had a higher solid-liquid interfacial tension (determined by contact angle measurements), thus being more wettable and increasing the drag, which propagates into the liquid flow, decreasing polarization. PLF-FI can thus identify regions with different chemical constitutions.

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Orientational relaxation times of rhodamine 700 in glycerol-water mixtures

Cited by 21 publications

References 19 publications

Sensing Temperature in Vitro and in Cells Using a BODIPY Molecular Probe

Sensing Temperature in Vitro and in Cells Using a BODIPY Molecular Probe

Accurate Determination of the Limiting Anisotropy of Rhodamine 101. Implications for Its Use as a Fluorescence Polarization Standard

Detection of chemical alterations at internal walls of microchannel flow cells by nondestructive fluorescence depolarization

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