Orientation of collagen fibers at the boundary between two successive osteonic lamellae and its mechanical interpretation

Ascenzi, A.; Benvenuti, A.

doi:10.1016/0021-9290(86)90022-9

Cited by 66 publications

(34 citation statements)

References 8 publications

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“…These authors provided evidence that collagen fibers and mineral crystallites in a predominantly transverse orientation, perpendicular to the long axis of the bone, are better able to resist compressive forces, whereas longitudinal fibers and crystallites, oriented more parallel to the long axis of the bone, are better able to resist tensile forces. Collagen fibers oriented at 45 degrees between longitudinal and transverse lamellae were said to provide better resistance to shear (Ascenzi and Benvenuti, 1986). Collagen fibers of differing orientation can be visualized in polarized light, so that collagen fibers oriented transversely appear bright, those oriented longitudinally appear dark, and fibers having intermediate orientations appear in different levels of grey.…”

Section: Background Preferred Collagen Fiber Orientationmentioning

confidence: 99%

Circularly polarized light standards for investigations of collagen fiber orientation in bone

Bromage

Goldman²,

McFarlin

et al. 2003

The Anatomical Record Part B

214

211

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Bone exhibits positive form birefringence dominated by and dependent upon the orientation of its collagen. The biomechanical efficacy of bone as a tissue is largely determined by collagen fibers of preferred orientation and distribution (and corresponding orientation of mineral crystallites), and evidence is accumulating to demonstrate that this efficacy extends to function at the organ level. This study has three aims. The first is to provide a background to the study of circularly polarized light (CPL) investigations of collagen fiber orientation in bone. The significance of preferred collagen fiber orientation in bone, linearly polarized light and CPL imaging principles, and a short history of CPL studies of mammalian functional histology are reviewed. The second is to describe, in some detail, methodological considerations relating to specimen preparation and imaging appropriate for the quantitative analysis of preferentially oriented collagen. These include section transparency, section thickness, the uniformity of the illuminating system, and CPL paraphernalia. Finally, we describe a grey-level standard useful for quantitative CPL, based upon mineralized turkey tendon, which shall be provided to investigators upon request. When due consideration is paid to specimen preparation and imaging conditions, quantitative assessment of collagen fiber orientation provides insight into the effects of mechanical loading on the skeleton. Anat Rec (Part B: New Anat) 274B: 157-168, 2003.

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Section: Background Preferred Collagen Fiber Orientationmentioning

confidence: 99%

Circularly polarized light standards for investigations of collagen fiber orientation in bone

Bromage

Goldman²,

McFarlin

et al. 2003

The Anatomical Record Part B

214

211

View full text Add to dashboard Cite

show abstract

“…In a single osteonal lamellae, the previous mentioned sub-layers rotate to an angle from roughly 10° to 60° in respect to osteon long axis (Wagermaier et al, 2006). Finally, between two subsequent lamellae lies a putatively NCP-rich (Derkx et al, 1998) interlamellar area where the mineralized collagen fibrils are oriented perpendicularly to the longitudinal axis of the osteon (Ascenzi and Benvenuti, 1986;Reid, 1986;Ziv et al, 1996). This area is often called "thin", "dense" or "transverse" lamella (Marotti, 1993;Reid, 1986), and has been characterized as a transition zone between lamellae (Ziv et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Load-bearing in cortical bone microstructure: Selective stiffening and heterogeneous strain distribution at the lamellar level

Katsamenis

Chong

Andriotis

et al. 2013

Journal of the Mechanical Behavior of Biomedical Materials

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An improved understanding of bone mechanics is vital in the development of evaluation strategies for patients at risk of bone fracture. The current evaluation approach based on bone mineral density (BMD) measurements lacks sensitivity, and it has become clear that as well as bone mass, bone quality should also be evaluated.The latter includes, among other parameters, the bone matrix material properties, which in turn depend on the hierarchical structural features that make up bone as well as their composition. Optimal load transfer, energy dissipation and toughening mechanisms have, to some extent, been uncovered in bone. Yet, the origin of these properties and their dependence upon the hierarchical structure and composition of bone are largely unknown. Here we investigate load transfer in the osteonal and subosteonal levels and the mechanical behaviour of osteonal lamellae and interlamellar areas during loading. Using cantilever-based nanoindentation, in situ microtensile testing during atomic force microscopy (AFM) and digital image correlation (DIC), we report evidence for a previously unknown mechanism. This mechanism transfers load and movement in a manner analogous to the engineered "elastomeric bearing pads" used in large engineering structures. ȝ-RAMAN microscopy investigations 2 showed compositional differences between lamellae and interlamellar areas. The latter have lower collagen content but an increased concentration of noncollagenous proteins (NCPs). Hence, NCPs enriched areas on the microscale might be similarly important for bone failure as on the nanoscale. Finally, we managed to capture stable crack propagation within the interlamellar areas in a time-lapsed fashion, proving their significant contribution towards fracture toughness.

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“…More recent studies indicate that the fibrils are all more or less parallel to the interlamellar boundaries and that fibril directions are nearly orthogonal to each other in adjacent lamellae (Ascenzi and Bonucci, 1976;Reid, 1986). However, studies of transition zones between lamellae have shown crisscrossed oblique collagen fibrils (Ascenzi and Benvenuti, 1986) and regions of gradually rotating fibril orientations, with the latter present in the same bone together with regions of sharp interlamellar transitions (GiraudGuille, 1988). Other less widely accepted proposals for lamellar structure envisage the lamellae arising from alternate dense and loose layers, both oriented in many subsequently mineralize.…”

Section: Introductionmentioning

confidence: 95%

Transitional structures in lamellar bone

Ziv

Sabanay

Arad

et al. 1996

Microsc. Res. Tech.

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Orientation of collagen fibers at the boundary between two successive osteonic lamellae and its mechanical interpretation

Cited by 66 publications

References 8 publications

Circularly polarized light standards for investigations of collagen fiber orientation in bone

Circularly polarized light standards for investigations of collagen fiber orientation in bone

Load-bearing in cortical bone microstructure: Selective stiffening and heterogeneous strain distribution at the lamellar level

Transitional structures in lamellar bone

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