Oridonin relieves hypoxia-evoked apoptosis and autophagy via modulating microRNA-214 in H9c2 cells

Abstract

Oridonin (Orid) has been diffusely applied to remedy dissimilar cancers. Howbeit, the influence of Orid in ischemic heart disease (IHD) remains imprecise. The current study uncovered the functions of Orid in hypoxia-caused apoptosis and autophagy in H9c2 cells. H9c2 cells received hypoxia and Orid manipulation, cell viability, apoptosis, apoptosis-interrelated factors and autophagy-correlative factors were appraised. After the extraordinary vectors transfections, the impacts of miR-214 inhibition on hypoxia-triggered apoptosis and autophagy were investigated. Further, dual luciferase reporter assay was enforced for ascertaining the pertinence between miR-214 and PTEN. PI3K/AKT/mTOR pathway was finally determined using western blot. We found that, Orid significantly alleviated hypoxia-induced apoptosis and autophagy through regulation their associated proteins in H9c2 cells. Up-regulation of miR-214 was found in hypoxia and Orid co-managed cells, meanwhile, repression of miR-214 obviously annulled the modulatory functions of Orid in hypoxia-evoked apoptosis and autophagy. Additionally, PTEN was forecasted to be a firsthand target of miR-214. Besides, we observed that Orid evoked PI3K/ AKT/mTOR activation through elevation of miR-214 in hypoxia-managed H9c2 cells. In conclusion, the amusing results corroborated that Orid relieved hypoxia-caused apoptosis and autophagy via adjusting PI3K/AKT/mTOR pathway through enhancement of miR-214 in H9c2 cells.

miR-214-3p Attenuates Sepsis-Induced Myocardial Dysfunction in Mice by Inhibiting Autophagy through PTEN/AKT/mTOR Pathway

“…Gong et al reported that Oridonin relieved hypoxia-caused apoptosis and autophagy via adjusting the PI3K/AKT/mTOR pathway through the enhancement of miR-214 in H9C2 cells. Additionally, PTEN was forecasted to be a first-hand target of miR-214 [ 27 ]. Hu et al reported that miR-214 promotes radio sensitivity by the inhibition of ATG12-mediated autophagy in colorectal cancer (CRC).…”

Oridonin prevents oxidative stress‐induced endothelial injury via promoting Nrf‐2 pathway in ischaemic stroke

“…have found oridonin could relieve hypoxia‐induced apoptosis in H9C2 cells. 40 Basically, we observed the protective effects of oridonin on endothelial cells apoptosis both in vivo and in vitro. The increased colocation of IB4 + endothelial cells and TUNEL + apoptotic cells in vehicle‐treatment tMCAO group was reversed with oridonin treatment, and in vitro, FACS results of bEND.3 cells after OGD/R also showed oridonin significantly inhibited the cell apoptosis in a concentration‐dependent manner.…”

“…have found oridonin could relieve hypoxia-induced apoptosis in H9C2 cells. 40 Emerging reports suggest that Nrf-2, as a vital nuclear transcriptional factor, shows strong anti-oxidative activity and has been widely known as a promoter to inhibit oxidative stress and the resulting inflammation. 41 Especially, the increase in Nrf-2 nuclear translocation is strongly associated with anti-oxidative stress injury via activating AKT(Ser473)/GSK3β(Ser473)/Fyn signalling pathway, 42,43 and reportedly, oridonin could exert protective effects on anti-inflammatory and anti-oxidative activities via modulating Nrf-2.…”

MicroRNA‑214 promotes the EMT process in melanoma by downregulating CADM1 expression

“…mir-214 functions as a pro-metastatic factor in malignant tumors (42), and plays a critical role in the migration and invasion of cancer cells (43)(44)(45). The present study identified a potential link between mir-214 and cadM1 in the coordination of metastasis in melanoma.…”