Oridonin Enhances the Anti-Metastasis Effect of Oxaliplatinliplatin on Colorectal Cancer Liver Metastasis

Wen, Dan; Yang, Yu-Shen; Gao, Dazhi; Wang, Z; Jiang, Qi; Zhao, Xue-Feng

doi:10.1007/s10517-021-05324-y

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…In recent years, numerous studies have demonstrated that oridonin has a vital role in anti-inflammatory [6], anti-cancer [7,8], antibacterial [9], anti-sepsis [10], neuroprotection [11], immune regulation and other pharmacological and physiological aspects [12]. Among them, its anticancer activity has been widely reported in nasopharyngeal cancer [13], esophageal cancer [14], gastric cancer [15], colorectal cancer [16,17], pancreatic cancer [18], gallbladder cancer [19], breast cancer [20], ovarian cancer and other malignant cancers [21,22]. And the anticancer mechanism of oridonin is involved with inhibition of proliferation, induction of apoptosis, autophagy and inhibition of migration and invasion [23].…”

Section: Ivyspringmentioning

confidence: 99%

Oridonin inhibited epithelial-mesenchymal transition of laryngeal carcinoma by positively regulating LKB1/AMPK signaling

Kou,

Shi,

Zhou

et al. 2024

Int. J. Med. Sci.

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Oridonin is the main bioactive component of Rabdosia rubescens, and its anticancer activity has been reported in a variety of cancers. However, the molecular mechanism of oridonin in laryngeal carcinoma remains unclear. In the present study, the cytotoxic effect of oridonin on laryngeal carcinoma Hep-2 and TU212 cell lines were initially detected by modified MTT assay. The results showed that oridonin had a dose-dependent anti-proliferative effect on laryngeal carcinoma Hep-2 and TU212 cells. Next, we found that oridonin significantly inhibited the migration and invasion of human laryngeal carcinoma Hep-2 and TU212 cell lines by wound healing assay and transwell assay. Subsequently, the results of quantitative real-time PCR assay and western blotting assay confirmed that oridonin upregulated the expression of E-cadherin while downregulated the expression of N-cadherin in a concentration-dependent manner at mRNA and protein levels. In addition, phosphorylation levels of liver kinase B1 (p-LKB1) and AMP-activated protein kinase (p-AMPK) were also elevated upon oridonin treatment. To further verify the role of LKB1/AMPK signaling pathway in laryngeal carcinoma, overexpression of LKB1 was constructed by plasmid transfection. The data exhibited that overexpression of LKB1 could further reinforce the increase of E-cadherin level and decrease of N-cadherin level mediated by oridonin. Additionally, AMPK inhibitor compound C could reverse anti-metastatic effect of oridonin on laryngeal carcinoma, and antagonise EMT expression. In contrast, AMPK activator AICAR presented the opposite effect. In conclusion, our study revealed that oridonin could remarkably reverse the epithelial-mesenchymal transition of laryngeal carcinoma by positively regulating LKB1/AMPK signaling pathway, which suggested that oridonin may be a potential candidate for the treatment of laryngeal carcinoma in the future.

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Section: Ivyspringmentioning

confidence: 99%

Oridonin inhibited epithelial-mesenchymal transition of laryngeal carcinoma by positively regulating LKB1/AMPK signaling

Kou,

Shi,

Zhou

et al. 2024

Int. J. Med. Sci.

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“…Oridonin, a natural terpenoids found in traditional Chinese herbal medicine, impedes inflammasome assembly by forming covalent bond with NLRP3 Cys279 (72). Oridonin administration effectively prevents the formation of colorectal cancer liver metastasis (73) and improves oxaliplatin efficacy (74). Oridonin derivative, with potent anticancer effects, has been very recently synthesized (75).…”

Section: Inflammasome Targeting Drugs: Potential Anti-cancer Therapeuticmentioning

confidence: 99%

Extracellular Vesicles and the Inflammasome: An Intricate Network Sustaining Chemoresistance

et al. 2022

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Extracellular vesicles (EVs) are membrane enclosed spherical particles devoted to intercellular communication. Cancer-derived EVs (Ca-EVs) are deeply involved in tumor microenvironment remodeling, modifying the inflammatory phenotype of cancerous and non-cancerous residing cells. Inflammation plays a pivotal role in initiation, development, and progression of many types of malignancies. The key feature of cancer-related inflammation is the production of cytokines that incessantly modify of the surrounding environment. Interleukin-1β (IL-1β) is one of the most powerful cytokines, influencing all the initiation-to-progression stages of many types of cancers and represents an emerging critical contributor to chemoresistance. IL-1β production strictly depends on the activation of inflammasome, a cytoplasmic molecular platform sensing exogenous and endogenous danger signals. It has been recently shown that Ca-EVs can activate the inflammasome cascade and IL-1β production in tumor microenvironment-residing cells. Since inflammasome dysregulation has been established as crucial regulator in inflammation-associated tumorigenesis and chemoresistance, it is conceivable that the use of inflammasome-inhibiting drugs may be employed as adjuvant chemotherapy to counteract chemoresistance. This review focuses on the role of cancer-derived EVs in tuning tumor microenvironment unveiling the intricate network between inflammasome and chemoresistance.

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“…Oridonin has previously been investigated for its therapeutic effects on the following types of caners: colon, 2 , 3 , 6 , 10 , 11 breast, 12 , 13 pancreatic, 14 , 15 lung, 16 , 17 , 18 gallbladder, 19 prostate, 20 ovarian, 21 oesophageal, 22 oral, 23 , 24 bladder, 25 liver 26 and other cancers. It has been reported that the inhibitory effect of oridonin on proliferation of gastric cancer is mainly related to inducing apoptosis and promoting autophagy, 27 , 28 , 29 without further in‐depth discussions.…”

Section: Introductionmentioning

confidence: 99%

Oridonin suppresses gastric cancer SGC‐7901 cell proliferation by targeting the TNF‐alpha/androgen receptor/TGF‐beta signalling pathway axis

Gao

Tan

2023

J Cellular Molecular Medi

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Statistics provided by GLOBOCAN list gastric cancer as the sixth most common, with a mortality ranking of third highest for the year 2020. In China, a herb called Rabdosia rubescens (Hemsl.) H.Hara, has been used by local residents for the treatment of digestive tract cancer for hundreds of years. Oridonin, the main ingredient of the herb, has a curative effect for gastric cancer, but the mechanism has not been previously clarified. This study mainly aimed to investigate the role of TNF‐alpha/Androgen receptor/TGF‐beta signalling pathway axis in mediating the proliferation inhibition of oridonin on gastric cancer SGC‐7901 cells. MTT assay, cell morphology observation assay and fluorescence assay were adopted to study the efficacy of oridonin on cell proliferation. The network pharmacology was used to predict the pathway axis regulated by oridonin. Western blot assay was adopted to verify the TNF‐α/Androgen receptor/TGF‐β signalling pathway axis regulation on gastric cancer by oridonin. The results showed Oridonin could inhibit the proliferation of gastric cancer cells, change cell morphology and cause cell nuclear fragmentation. A total of 11signaling pathways were annotated by the network pharmacology, among them, Tumour necrosis factor alpha (TNF‐α) signalling pathway, androgen receptor (AR) signalling pathway and transforming growth factor (TGF‐β) signalling pathway account for the largest proportion. Oridonin can regulate the protein expression of the three signalling pathways, which is consistent with the results predicted by network pharmacology. These findings indicated that oridonin can inhibit the proliferation of gastric cancer SGC‐7901 cells by regulating the TNF‐α /AR /TGF‐β signalling pathway axis.

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Oridonin Enhances the Anti-Metastasis Effect of Oxaliplatinliplatin on Colorectal Cancer Liver Metastasis

Cited by 3 publications

References 14 publications

Oridonin inhibited epithelial-mesenchymal transition of laryngeal carcinoma by positively regulating LKB1/AMPK signaling

Oridonin inhibited epithelial-mesenchymal transition of laryngeal carcinoma by positively regulating LKB1/AMPK signaling

Extracellular Vesicles and the Inflammasome: An Intricate Network Sustaining Chemoresistance

Oridonin suppresses gastric cancer SGC‐7901 cell proliferation by targeting the TNF‐alpha/androgen receptor/TGF‐beta signalling pathway axis

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