Orgyia Pseudotsugata Baculovirus p10 and Polyhedron Envelope Protein Genes: Analysis of their Relative Expression Levels and Role in Polyhedron Structure

Gross, Christian H.; Russell, Rebecca L. Q.; Rohrmann, George F.

doi:10.1099/0022-1317-75-5-1115

Cited by 81 publications

(66 citation statements)

References 15 publications

(30 reference statements)

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“…The mature bigger polyhedron is present in the nucleus or extracellular medium and has an envelope, which confers its defined geometric shape ( Figure 5). The polyhedron envelope or "calyx" seems to have two roles: to prevent the loss of virions, and to avoid polyhedron aggregation (Gross et al, 1994). In the electron micrographs, the polyhedron envelope has a membrane-like structure, as shown in Figure 6.…”

Section: Resultsmentioning

confidence: 99%

Cytopathology of Bombyx mori (Lepidoptera: Bombycidae) silk gland caused by multiple nucleopolyhedrovirus

Brancalhão

Torquato²,

Fernandez³

2009

Genet. Mol. Res.

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ABSTRACT.A multiple nucleopolyhedrovirus previously isolated from infected Bombyx mori L. larvae (BmMNPV) in Paraná State, Brazil, was inoculated into B. mori larvae to examine susceptibility and cytopathology in silk gland cells. The anterior, middle and posterior silk glands were removed from the infected silkworm at different times post-inoculation and processed for cytopathology studies by light and transmission electron microscopy. BmMNPV infection was only detected at 72 h post-inoculation in cells of the middle and posterior silk glands. No sign of infection was found in the anterior silk gland. Cytopathological characteristics were similar to those found in other target tissues; initially, they consisted of nuclear hypertrophy and the formation of virogenic stroma (viroplasm), in which the progeny virions are produced. The virions are then enveloped and occluded in protein crystal structures, the polyhedra. After viral replication, cells undergo lysis and release a great number of BmMNPV polyhedra into the hemocoele. Histopathology showed early infection foci in regions surrounding trachea insertions, demonstrating that trachea is an infection-spreading organ in the insect body. Trachea penetrates the middle and posterior silk gland basal lamina, considered a barrier to virus, facilitating the penetration of budded virus. The anterior silk gland does not have tracheal insertions into the basal lamina, which reduces budded virus infection. Tracheal branches provide a conduit for non-occluded virions (budded virus) to pass through the basal lamina barrier and disseminate BmMNPV in the silkworm silk gland.

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Section: Resultsmentioning

confidence: 99%

Cytopathology of Bombyx mori (Lepidoptera: Bombycidae) silk gland caused by multiple nucleopolyhedrovirus

Brancalhão

Torquato²,

Fernandez³

2009

Genet. Mol. Res.

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“…Brancalhão et al (2009) classified these polyhedra as immature and mature, respectively; the geometric shape is provided by the envelope. The envelope or "calyx" acts to protect the virions, preventing their loss to the external environment (GROSS et al, 1994) and prevents the aggregation of the polyhedra (LIANG et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Ampullae infection of the malpighian tubules of Bombyx mori by Alphabaculovirus

Santorum¹,

Beu²,

Ribeiro³

et al. 2017

Biosci. J.

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ABSTRACT:Alphabaculovirus is a genus of the entomopathogenic virus, whose species Bombyx mori nucleopolyhedrovirus (BmNPV) infects the silkworm, Bombyx mori, which is an important insect in the sericulture industry. A geographic isolate of BmNPV was identified in the state of Paraná, Brazil. It was infecting B. mori larvae and various organs and target tissues were identified, however, there was no information about the infection of Malpighian tubules (MT). The MT comprises the excretory system of B. mori and acts in the elimination of toxic substances and in hydroelectrolytic homeostasis. Thus, the present study examined the susceptibility and cytopathology of B. mori MT to BmNPV. To this end, hybrid fifth instar larvae were inoculated with a virus suspension at different days post-inoculation (dpi). MT segments were collected and divided into the ampullae, proximal, medial and distal regions. These were processed for light microscopy and transmission electron analysis. The MT regions revealed differences in susceptibility to BmNPV and the ampullae in its transition area was infected from the sixth dpi; the other regions did not reveal any evidence of infection. The transition area of the ampullae has not been previously described in Lepidoptera and its cytopathology revealed a hypertrophic nucleus with viroplasm, followed by the formation and development of viral polyhedra, which are common characteristics of infections by Alphabaculovirus. Thus, infection of the ampullae of the MT of B. mori by BmNPV, together with other known targets, compromises the metabolic balance of the insect, which results in consequences for silk production and damage to the sericulture sector.

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“…A P10 é uma proteína com massa molecular de 10 kDa, que está envolvida no processo de oclusão das partículas virais e provavelmente na lise celular no final da infecção (Van Oers et al, 1994). Também em estudos com a construção de vírus recombinantes sem o gene p10 foi demonstrado que essa proteína é importante para manter a integridade do poliedro (Vlak et al, 1988;Williams et al, 1989;Gross et al,1994).…”

Section: Fase L (Late)unclassified

Molecular biology of baculovirus and its use in biological control in Brazil

Castro

Souza

Sihler

et al. 1999

Pesq. agropec. bras.

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4RESUMO -Os baculovírus são vírus patogênicos a insetos, encontrados principalmente na ordem Lepidoptera. A família Baculoviridae é taxonomicamente dividida em dois gêneros: Nucleopolyhedrovirus e Granulovirus, que diferem pela morfologia do corpo de oclusão. Os nucleopoliedrovírus (NPV) possuem corpos de inclusão poliédrica (PIB), contendo múltiplas partículas virais, enquanto os granulovírus (GV) contêm, em geral, partículas únicas, ocluídas em corpos protéicos de forma ovóide. Durante o ciclo de vida são produzidos dois tipos de progênies virais: BV (Budded Virus) e PDV (Polyhedra Derived Virus), que são essenciais para o processo de infecção e propagação do vírus. Os baculovírus têm sido empregados no controle de pragas e, por serem específicos e restritos a invertebrados, são considerados agentes seguros de controle biológico. Recentemente têm sido amplamente utilizados como vetor de expressão de genes heterólogos por produzirem e processarem, em grande quantidade, proteínas de procariotos e eucariotos. Além disso, técnicas de DNA recombinante têm permitido a produção de inseticidas virais geneticamente modificados. Este trabalho constitui uma revisão sobre a taxonomia, estrutura, replicação e biologia molecular de baculovírus e sobre seu uso como bioinseticida no Brasil.

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Orgyia Pseudotsugata Baculovirus p10 and Polyhedron Envelope Protein Genes: Analysis of their Relative Expression Levels and Role in Polyhedron Structure

Cited by 81 publications

References 15 publications

Cytopathology of Bombyx mori (Lepidoptera: Bombycidae) silk gland caused by multiple nucleopolyhedrovirus

Cytopathology of Bombyx mori (Lepidoptera: Bombycidae) silk gland caused by multiple nucleopolyhedrovirus

Ampullae infection of the malpighian tubules of Bombyx mori by Alphabaculovirus

Molecular biology of baculovirus and its use in biological control in Brazil

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