Organs-on-chip monitoring: sensors and other strategies

Kilic, Tugba; Navaee, Fatemeh; Stradolini, Francesca; Renaud, Philippe; Carrara, Sandro

doi:10.21037/mps.2018.01.01

Cited by 79 publications

(98 citation statements)

References 153 publications

(234 reference statements)

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“… Example uses of lab on-a-chip systems. All figures within this figure were prepared on the basis of original papers (body [ 15 ], brain [ 16 ], gut [ 17 ], heart [ 18 ], kidney [ 19 ], liver [ 20 ], lung [ 21 ], lymph node [ 22 ], and skin [ 23 ]). …”

Section: Figurementioning

confidence: 99%

Novel Strategies in Artificial Organ Development: What Is the Future of Medicine?

Klak¹,

Bryniarski²,

Kowalska³

et al. 2020

Micromachines

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The technology of tissue engineering is a rapidly evolving interdisciplinary field of science that elevates cell-based research from 2D cultures through organoids to whole bionic organs. 3D bioprinting and organ-on-a-chip approaches through generation of three-dimensional cultures at different scales, applied separately or combined, are widely used in basic studies, drug screening and regenerative medicine. They enable analyses of tissue-like conditions that yield much more reliable results than monolayer cell cultures. Annually, millions of animals worldwide are used for preclinical research. Therefore, the rapid assessment of drug efficacy and toxicity in the early stages of preclinical testing can significantly reduce the number of animals, bringing great ethical and financial benefits. In this review, we describe 3D bioprinting techniques and first examples of printed bionic organs. We also present the possibilities of microfluidic systems, based on the latest reports. We demonstrate the pros and cons of both technologies and indicate their use in the future of medicine.

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Section: Figurementioning

confidence: 99%

Novel Strategies in Artificial Organ Development: What Is the Future of Medicine?

Klak¹,

Bryniarski²,

Kowalska³

et al. 2020

Micromachines

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“…Many of these systems incorporate biophysical and/or biochemical stimuli (i.e., mechanical [ 22 , 34 ], electrical [ 40 ] and biochemical cues [ 41 ]) to mimic the 3D in vivo physiological environment of the corresponding native organ and to induce the proper cellular phenotypes and tissue maturation. However, recapitulating and monitoring in a single system the controlled and dynamic exchange of molecules and dissolved gases, the complicated tissue-tissue interactions, as well as the complex tissue pathophysiology is still an open challenge [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

“…Major progresses in this field are related to the integration of biosensors for continuous, noninvasive, and on-line monitoring of parameters of interest. This, together with the possibility of parallelizing samples analysis characteristic of OoC, holds the promise to improve the exploitation of these in vitro systems as preclinical models and drug screening platforms [ 42 , 43 ]. Specifically, a range of electrical, magnetic, and optical biosensing approaches have been reported over the last years and previously reviewed [ 42 , 43 , 44 , 45 ] for monitoring cell populations within microfabricated OoC systems, with high sensitivity and high resolution [ 46 , 47 , 48 , 49 ].…”

Section: Introductionmentioning

confidence: 99%

“…This, together with the possibility of parallelizing samples analysis characteristic of OoC, holds the promise to improve the exploitation of these in vitro systems as preclinical models and drug screening platforms [ 42 , 43 ]. Specifically, a range of electrical, magnetic, and optical biosensing approaches have been reported over the last years and previously reviewed [ 42 , 43 , 44 , 45 ] for monitoring cell populations within microfabricated OoC systems, with high sensitivity and high resolution [ 46 , 47 , 48 , 49 ]. For instance, biosensors for monitoring cell growth and behavior [ 50 ], electrical [ 51 , 52 , 53 ] and mechanical [ 30 , 54 , 55 ] properties, as well as environmental parameters such as oxygen [ 56 , 57 , 58 ], pH [ 59 ], and metabolites concentration [ 60 , 61 ] have been studied and validated [ 62 ].…”

Section: Introductionmentioning

confidence: 99%

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Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

et al. 2020

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Organs-on-chip (OoC), often referred to as microphysiological systems (MPS), are advanced in vitro tools able to replicate essential functions of human organs. Owing to their unprecedented ability to recapitulate key features of the native cellular environments, they represent promising tools for tissue engineering and drug screening applications. The achievement of proper functionalities within OoC is crucial; to this purpose, several parameters (e.g., chemical, physical) need to be assessed. Currently, most approaches rely on off-chip analysis and imaging techniques. However, the urgent demand for continuous, noninvasive, and real-time monitoring of tissue constructs requires the direct integration of biosensors. In this review, we focus on recent strategies to miniaturize and embed biosensing systems into organs-on-chip platforms. Biosensors for monitoring biological models with metabolic activities, models with tissue barrier functions, as well as models with electromechanical properties will be described and critically evaluated. In addition, multisensor integration within multiorgan platforms will be further reviewed and discussed.

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“…Downscaling chemical and biological sensors result not only in ultra-portable devices, but in advantages such as enhanced process performance, higher analysis speed and reduced reagent consumption, significantly lowering fabrication, maintenance, and operational costs (Shakoor et al 2018). Some fields that benefit from these achievements are healthcare monitoring (Boppart and Richards-Kortum 2014; Wessels and Raad 2016;Zi et al 2016;Papageorgiou et al 2017;Sharma et al 2018), organ-on-a-chip (Zhang et al 2015;Si Hadj Mohand et al 2017;Kilic et al 2018), drug screening (Zhang et al 2015), food monitoring (Bhattacharya et al 2017), and environment monitoring (Ricciardi et al 2015a;Calvé et al 2017;Rezende et al 2019;Măriuța et al 2020). Potential applications are countless with a huge impact on the quality of life (Chen et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Miniaturization of fluorescence sensing in optofluidic devices

Măriuța

Colin

Barrot-Lattes

et al. 2020

Microfluid Nanofluid

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Successful development of a micro-total-analysis system (µTAS, lab-on-a-chip) is strictly related to the degree of miniaturization, integration, autonomy, sensitivity, selectivity, and repeatability of its detector. Fluorescence sensing is an optical detection method used for a large variety of biological and chemical assays, and its full integration within lab-on-a-chip devices remains a challenge. Important achievements were reported during the last few years, including improvements of previously reported methodologies, as well as new integration strategies. However, a universal paradigm remains elusive. This review considers achievements in the field of fluorescence sensing miniaturization, starting from off-chip approaches, representing miniaturized versions of their lab counter-parts, continuing gradually with strategies that aim to fully integrate fluorescence detection on-chip, and reporting the results around integration strategies based on optical-fiber-based designs, optical layer integrated designs, CMOS-based fluorescence sensing, and organic electronics. Further successful development in this field would enable the implementation of sensing networks in specific environments that, when coupled to Internet-of-Things (IoT) and artificial intelligence (AI), could provide real-time data collection and, therefore, revolutionize fields like health, environmental, and industrial sensing.

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Organs-on-chip monitoring: sensors and other strategies

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Cited by 79 publications

References 153 publications

Novel Strategies in Artificial Organ Development: What Is the Future of Medicine?

Novel Strategies in Artificial Organ Development: What Is the Future of Medicine?

Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

Miniaturization of fluorescence sensing in optofluidic devices

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