Organophosphorus insecticides mineralizing endophytic and rhizospheric soil bacterial consortium influence eggplant growth-promotion

Unveiling chlorpyrifos mineralizing and tomato plant-growth activities of Enterobacter sp. strain HSTU-ASh6 using biochemical tests, field experiments, genomics, and in silico analyses

“…The present study assumed that the strain HSTU-ASh6 could fix nitrogen in Jensen's medium. Phosphate-solubilizing bacteria can dissolve insoluble phosphate and increase soil fertility and plant growth (Loiret et al, 2004;Das et al, 2022), which was also demonstrated by the strain HSTU-ASh6. Moreover, the strain enhanced the germination rate and increased tomato plant growth at vegetative and reproductive stages when using 70 and 30% reduced doses of urea.…”

“…They consume almost all-natural and synthetic compounds as their source of carbon and energy. Moreover, a significant number of genes such as opd (organophosphate degrading), carboxylesterase, mpd (methyl parathion degrading), and amidohydrolase, α/β hydrolase and several enzymes are known to be involved in degrading certain organophosphate pesticides ( Yang et al, 2006 ; Parakhia et al, 2014 ; Lee et al, 2021 ; Das et al, 2022 ).…”

Fate of heavy metals in Swarna rice after traditional cooking and submerged fermentation linked to bacterial interactions

“…The isolates were biochemically characterized according to the results of oxidase, catalase, methyl red (MR), Voges-Proskauer (VP), triple sugar iron (TSI), citrate utilization (CU), indole, motility indole urease (MIU), and carbohydrates (lactose, maltose, sucrose and dextrose) fermentation tests in aseptic condition (Abdullah-Al-Mamun et al, 2022;Haque et al, 2015a). The activities of the extracellular amylase, pectinase, xylanase, and protease of the isolates were conducted by spreading the bacterial samples with a sterile loop in starch, pectin, xylan, and casein medium that served as the sole carbon source in an aseptic condition as described (Das et al, 2022;Haque et al, 2021). The agar plates were streaked by picking off a loopful colony of 24 h pure culture of the isolates.…”

“…The agar plates were streaked by picking off a loopful colony of 24 h pure culture of the isolates. The culture plates were incubated at 37 °C for 24 h. Finally, the plates (starch, pectin, xylan, casein) were washed with Congo red followed by distilled water, and the hydrolytic zone was observed as described (Das et al, 2022).…”

“…The PCR amplification was carried out in a 200 µL PCR tube containing 25 µL of 2X PCR Master mix solution (Promega Corporation, USA), 27F (5'-AGA GTT TGT TGA TGG CTC AG -3') as the forward primer (5.0 µL), and 1492R (5'-GGT TAC CTT GTT ACG ACT T-3') as the reverse primer (5.0 µL), template DNA 2.0 µL, and a nuclease-free water 13.0 µL. The following PCR steps were programmed, visualized, purified, and sequenced for gene amplification, (Haque et al, 2021;Das et al, 2022). The query sequence was used to construct a phylogenetic tree using MEGA 7.0 software with the neighbor-joining method (bootstrap 1000x).…”