Organophosphorous Pesticide Exposure Increases the Frequency of Sperm Sex Null Aneuploidy

Recio, R; Robbins, W. A.; Ocampo-Gomez, Guadalupe; Borja-Aburto, Víctor Hugo; Morán-Martínez, Javier; Froines, John R.; Hernandez, Rosa Ma. Garcia; Cebrián, Mariano E.

doi:10.2307/3454745

Cited by 35 publications

(39 citation statements)

References 20 publications

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“…Epidemiological studies have linked exposure to OP (including Me-Pa) with decreased semen quality, increased sperm DNA damage and hormonal imbalance (Padungtod et al, 2000;Recio et al, 2001Recio et al, , 2005Sánchez-Peña et al, 2004). In addition, Me-Pa exposure caused testicular dysfunction (Narayana, 2006), reduced sperm quality (Mathew et al, 1992;Narayana et al, 2005), and increased sperm genetic damage (Narayana et al, 2005;Piña-Guzmán et al, 2006) in laboratory animals.…”

Section: Discussionmentioning

confidence: 99%

Methyl-parathion decreases sperm function and fertilization capacity after targeting spermatocytes and maturing spermatozoa

Piña-Guzmán

Sánchez-Gutiérrez

Marchetti

et al. 2009

Toxicology and Applied Pharmacology

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Paternal germline exposure to organophosphorous pesticides (OP) has been associated with reproductive failures and adverse effects in the offspring. Methyl parathion (Me-Pa), a worldwide-used OP, has reproductive adverse effects and is genotoxic to sperm.Oxidative damage has been involved in the genotoxic and reproductive effects of OP.The purpose of this study was to determine the effects of Me-Pa on spermatozoa function and ability to fertilize. Male mice were exposed to i.p.) and spermatozoa from epididymis-vas deferens were collected at 7 or 28 days posttreatment (dpt) to assess the effects on maturing spermatozoa and spermatocytes, respectively. DNA damage was evaluated by nick translation (NT-positive cells) and SCSA (%DFI); lipoperoxidation (LPO) by malondialdehyde production; sperm function by spontaneous-and induced-acrosome reactions (AR); mitochondrial membrane potential (MMP) by using the JC-1 flurochrome; and, fertilization ability by an in vitro assay and in vivo mating. Results showed alterations in DNA integrity (%DFI and NTpositive cells) at 7 and 28 dpt, in addition to decreased sperm quality and a decrease in induced-AR; reduced MMP and LPO was observed only at 7 dpt. We found negative correlations between LPO and all sperm alterations. Altered sperm functional parameters were associated with reduced fertilization rates at both times, evaluated either in vitro or in vivo. These results show that Me-Pa exposure of maturing spermatozoa and spermatocytes affects many sperm functional parameters that result in a decreased fertilizing capacity. Oxidative stress seems to be a likely mechanism of the detrimental effects of Me-Pa in male germ cells.Key words. methyl-parathion, sperm function, fertilization, oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Methyl-parathion decreases sperm function and fertilization capacity after targeting spermatocytes and maturing spermatozoa

Piña-Guzmán

Sánchez-Gutiérrez

Marchetti

et al. 2009

Toxicology and Applied Pharmacology

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“…Padungtod et al reported that exposure to methyl parathion, ethyl parathion and methamidophos in Chinese pesticide factory workers had a small effect on male reproductive hormones 17) and increased the prevalence of sperm aneuploidy 16) . Another study revealed a positive association between OP metabolite levels and sex null and total aneuploidy frequencies in Mexican agricultural workers 19) . The sprayers in the present study had been exposed to fenitrothion most frequently, the testicular toxicity of which has not been reported, and often sprayed it together with DDVP, which was reported to be a possible testicular toxin 27,28) and to cause retention of cytoplasmic droplet and reduce sperm motility 29) in animal studies.…”

Section: Discussionmentioning

confidence: 99%

“…Given that these pesticides could pose a possible risk for human reproduction, semen studies in pesticide-exposed workers have been conducted worldwide in recent years. Some of them suggested the deterioration of semen quality [16][17][18][19] , while others did not detect any significant alterations 20,21) . In the light of ongoing development of this research area, it is now necessary to accumulate more findings in various exposure settings.…”

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confidence: 99%

A Survey of Semen Indices in Insecticide Sprayers

Kamijima

Hibi

Gotoh

et al. 2004

Journal of Occupational Health

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A Survey of Semen Indices in InsecticideSprayers: Michihiro Kamijima, et al. Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine-This study aims at clarifying the semen indices of insecticide sprayers who are exposed mainly to organophosphorus and pyrethroid insecticides. Eighteen male sprayers out of 54 working for 9 companies in central Japan and 18 age-matched students or medical doctors as unexposed controls participated in detailed reproductive check-ups conducted in summer and the following winter. The sprayers were exposed to insecticides more in summer, the busiest season, than winter, the off-season (p<0.05). Erythrocyte true cholinesterase activities in the sprayers were lower than in the controls in summer (p<0.05), and decreased in significant association with the increase in exposure frequency. Testicular volumes in the sprayers tended to be smaller than in the controls (p=0.06). The serum testosterone concentration in winter in the sprayers was higher than in the controls (p<0.05), though luteinizing h o r m o n e a n d f o l l i c l e s t i m u l a t i n g h o r m o n e concentrations were not significantly different. The sperm counts and vitality were comparable between the groups, but detailed sperm motility analysis in summer revealed that the percentages of slow progressive and nonprogressive motile sperm were twice as high in the sprayers (p<0.05), and that of rapid progressive sperm tended to be lower (p=0.06). Such differences were not observed in winter. Differential sperm morphology counts showed that interaction of group and abstinence effects were significant in sperm Received Aug 19, 2003; Accepted Nov 28, 2003 Correspondence to: M. Kamijima, Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan (e-mail: kamijima@med.nagoya-u.ac.jp) with normal morphology and with head deformity only in the summer check-up. Despite possible inherent differences between the groups, the above seasondependent differences suggested that the observed lower semen quality in the sprayers was associated with pesticide spraying work.

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“…Pesticides in general may directly damage spermatozoa, alter Steroli cell or Laydig cell function, or disrupt the endocrine function in any stage of hormone regulation (hormone synthesis, release, storage, transport and clearance, receptor recognition and binding) [36]. Clear effects on male fertility have been demonstrated for some pesticides: dibromochloropropane [4], ethylene dibromide [37], organophosphorus [38,39], alochlor, metochlor, 2,4-D, atrazine [40], fenvalerate [41], carbaryl, chlorpyrifos [42]. The results of the recent studies performed in Denmark, China and Mexico indicated that exposure to pesticides (however not confirmed by exposure measurements) increased the risk of specific morphological abnormalities of the sperm and decreased sperm count per ejaculate and the percentage of viable sperm.…”

Section: Pesticidesmentioning

confidence: 99%

Environmental factors and semen quality

Jurewicz¹,

Hanke²,

Radwan³

et al. 2009

International Journal of Occupational Medicine and Environmental Health

160

109

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Objectives: An increasing number of reports suggest that chemical and physical agents in the environment, introduced and spread by human activity, may affect male fertility in humans. This article aims at evaluating the impact of environmental exposures (pesticides, phthalates, PCBs, air pollution, trihalomethanes (THMs), mobile phones) on semen quality, by reviewing most recent published literature. Materials and Methods: Epidemiological studies focusing on exposure to environmental factors and semen quality for the last ten years were identified by a search of the Pubmed, Medline, Ebsco, Agricola and Toxnet literature bases. Results: The results from the presented studies suggest that there are strong and rather consistent indications that some pesticides besides DBCP (e.g. DDT/Dichlorodiphenyldichloroethylene [DDE], ethylenedibromide, organophosphates) affects sperm count. PCBs are detrimental to sperm motility. In case of air pollution, studies suggest a link between ambient air pollutants and various semen characteristics. Additional research is needed to corroborate this association and to establish the causal agents. Results of few studies on subfertile men demonstrate associations between phthalate levels commonly experienced by the public and impaired sperm quality (impact on sperm concentration, morphology, motility), but the findings have not been corroborated in studies of men from the general population. Mobile phones might adversely affect the quality of semen by decreasing mostly motility but also the sperm counts, viability and morphology. In spite of their consistent results, most of the studies are rather small. Association between exposure to THMs and poor semen quality was not observed. Conclusions: Epidemiological studies suggest awareness of environmental factors which may affect semen quality. In case both of well proven and disputable reproductive and developmental hazards, it is necessary to prevent parental exposure to the agents associated with those hazards.

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Organophosphorous Pesticide Exposure Increases the Frequency of Sperm Sex Null Aneuploidy

Cited by 35 publications

References 20 publications

Methyl-parathion decreases sperm function and fertilization capacity after targeting spermatocytes and maturing spermatozoa

Methyl-parathion decreases sperm function and fertilization capacity after targeting spermatocytes and maturing spermatozoa

A Survey of Semen Indices in Insecticide Sprayers

Environmental factors and semen quality

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