Organophosphates of urine, including adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate

Price, T.D.; Ashman, D.F.; Melicow, Meyer M.

doi:10.1016/0005-2787(67)90542-4

Cited by 65 publications

(12 citation statements)

References 30 publications

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“…In 1964, an enzyme capable of breaking the 3',5'-phosphodiester bond of cGMP was characterized (Kuriyama et al, 1964). The same laboratory confirmed the detection of cGMP in rat urine and proposed that the cGMP synthesis is catalyzed by a cyclase (Price et al, 1967). The chemical and 3D structures of cGMP are shown in Fig.…”

Section: Discovering Camp In Higher Plantssupporting

confidence: 51%

Cyclic Nucleotides and Nucleotide Cyclases in Plant Stress Responses

Lemtiri‐Chlieh¹,

Thomas²,

Marondedze³

et al. 2011

Abiotic Stress Response in Plants - Physiological, Biochemical and Genetic Perspectives

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Section: Discovering Camp In Higher Plantssupporting

confidence: 51%

Cyclic Nucleotides and Nucleotide Cyclases in Plant Stress Responses

Lemtiri‐Chlieh¹,

Thomas²,

Marondedze³

et al. 2011

Abiotic Stress Response in Plants - Physiological, Biochemical and Genetic Perspectives

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“…Nephrogenous cyclic nucleotide can be detected by dilution of the urine cyclic nucleotide-3H specific radioactivity relative to that in plasma at infusion equilibrium and nmay be quantified as follows: rk = R, (3) where rk is the rate of nephrogenous cyclic nucleotide addition to the tubular urine; (Su).q and (S),eq are the cyclic nucleotide-3H specific radioactivities in urine and plasma at infusion equilibrium, and Re is the total rate of cyclic nucleotide excretion. In the infusion studies, where rk can be quantified, equation 2 may be corrected so that PRu yields an accurate estimate of cyclic nucleotide production rate in the miscible pool:…”

Section: Cyclic Amp and Cyclic Gmp In Man 2225mentioning

confidence: 99%

“…Adenosine 3',5'-monophosphate (cyclic AMP) and guanosine 3',5'-monophosphate (cyclic GMP) were identified in urine several years ago (1)(2)(3), but definitive studies concerning the sources of the excreted cyclic nucleotides have not been reported. Cyclic AMP has been found in dog plasma (1), and we have identified both cyclic nucleotides in human plasma, raising the possibility that renal plasma clearance could account for at least a portion of the cyclic nucleotides excreted into the urine.…”

Section: Introductionmentioning

confidence: 99%

Kinetic parameters and renal clearances of plasma adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate in man

Broadus¹,

Kaminsky²,

Hardman³

et al. 1970

J. Clin. Invest.

253

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A B S T R A C T Kinetic parameters and the renal clearances of plasma adenosine 3',5'-monophosphate (cyclic AMP) and guanosine 3',5'-monophosphate (cyclic GMP) were evaluated in normal subjects using tritium-labeled cyclic nucleotides. Each tracer was administered both by single, rapid intravenous injection and by constant intravenous infusion, and the specific activities of the cyclic nucleotides in plasma and urine were determined.Both cyclic AMP and cyclic GMP were cleared from plasma by glomerular filtration. The kidney was found to add a variable quantity of endogenous cyclic AMP to the tubular urine, amounting to an average of approximately one-third of the total level of cyclic AMP excreted. Plasma was the source of virtually all of the cyclic GMP excreted.Plasma levels of the cyclic nucleotides appeared to be in dynamic steady state. The apparent volumes of distribution of both nucleotides exceeded extracellular fluid volume, averaging 27 and 38% of body weight for cyclic AMP and cyclic GMP, respectively. Plasma production rates ranged from 9 to 17 nmoles/min for cyclic AMP and from 7 to 13 nnmoles/min for cyclic GMP. Plasma clearance rates averaged 668 mln/in for cyclic AMP and 855 ml/min for cyclic GMP. Approximately 85% of the elimination of the cyclic nucleotides from the circulation was due to extrarenal clearance.These studies were presented in part at the Annual Meeting of the Southern Society for Clinical Investigation, New Orleans, La., January, 1970. Clin. Res. 18: 73. (Abstr.) INTRODUCTION Adenosine 3',5'-monophosphate (cyclic AMP) and guanosine 3',5'-monophosphate (cyclic GMP) were identified in urine several years ago (1-3), but definitive studies concerning the sources of the excreted cyclic nucleotides have not been reported. Cyclic AMP has been found in dog plasma (1), and we have identified both cyclic nucleotides in human plasma, raising the possibility that renal plasma clearance could account for at least a portion of the cyclic nucleotides excreted into the urine. Two hormones known to stimulate renal adenyl cyclase systems (4) have been reported to increase cyclic AMP excretion (5, 6) suggesting the kidney as a source of urinary cyclic AMP. Similarly, the kidney has been suggested as a source of urinary cyclic GMP (7). In the absence of definitive clearance studies, it has not been possible to know whether the cyclic nucleotides in urine are derived solely from the kidney, solely from plasma, or both from plasma and from the kidney.The present studies were designed to evaluate the renal clearances as well as other kinetic parameters of the extracellular cyclic nucleotides. Tracer doses of tritiumlabeled cyclic nucleotides were administered to human subjects by rapid intravenous injection and by constant intravenous infusion. Specific radioactivity determinations of the cyclic nucleotides in plasma and urine provided information regarding the sources of cyclic AMP and cyclic GMP excreted into the urine. The effects of certain hormones on plasma and urinary cyclic nucleotides are repo...

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“…Guanosine 3',5'-monophosphate (cyclic GMP), which was first identified in urine (5,9,26,27), is the only cyclic nucleotide other than cyclic AMP known to occur in nature. The biological role of cyclic GMP remains to be established.…”

Section: Introductionmentioning

confidence: 99%