Organoids from mouse molar and incisor as new tools to study tooth-specific biology and development

Hermans, Florian; Hemeryck, Lara; Bueds, Celine; Pereiro, Marc Torres; Hasevoets, Steffie; Kobayashi, Hiroto; Lambrechts, Diether; Lambrichts, Ivo; Bronckaers, Annelies; Vankelecom, Hugo

doi:10.1016/j.stemcr.2023.03.011

Cited by 5 publications

(6 citation statements)

References 51 publications

(90 reference statements)

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“…Regenerative therapies have drawn attention as a solution to the issue. In the dental field, such therapeutic approaches include the use of synthetic materials [ 54 , 55 ], biological components [ [56] , [57] , [58] , [59] , [60] , [61] , [62] , [63] ], or even whole bioengineered teeth/tooth organoids [ [64] , [65] , [66] ] to recover dental structures.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Unraveling the hidden complexity: Exploring dental tissues through single-cell transcriptional profiling

Eldeeb,

Ikeda,

Hojo

et al. 2024

Regenerative Therapy

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Unraveling the hidden complexity: Exploring dental tissues through single-cell transcriptional profiling

Eldeeb,

Ikeda,

Hojo

et al. 2024

Regenerative Therapy

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“… Kim (2023) [ 89 ] Adult (9 week) mouse incisor organoids DMEM/F12 + 1X B27 , 1X N2 , N-acetylcysteine (1 mM), Y-27,632 (10 µM), 50% WNT3A conditioned medium, 5% RSPO1 conditioned medium, EGF (100 µg/mL), NOGGIN (200 µg/mL), FGF10 (100 µg/mL), dibenzazepine (10 mM), A83-01 (0.5 µM), nicotinamide (10 mM) day 0–14 : culture in expansion medium day 14–28 : suspension culture in expansion medium supplemented with SAG (concentration not provided by authors) Kidney transplantation : day 14 organoids were first cultured in suspension for 4 weeks and subsequently transplanted into the kidney capsule for 8 weeks. Underlined : Shared factor in all expansion media 1 SFDM: serum-free defined medium, see Hemeryck or Hermans et al for detailed composition [ 87 , 88 ]; 2 KSFM: keratinocyte serum-free medium …”

Section: Main Textmentioning

confidence: 99%

“… Underlined : Shared factor in all expansion media 1 SFDM: serum-free defined medium, see Hemeryck or Hermans et al for detailed composition [ 87 , 88 ]; 2 KSFM: keratinocyte serum-free medium …”

Section: Main Textmentioning

confidence: 99%

“…Mouse TO were established from early-postnatal (day 7) human-resembling molars and ever-growing incisors, and from 9-week-old adult mouse incisor [ 88 , 89 ]. Both protocols rely on typical organoid medium components containing WNT pathway activation (WNT3A, RSPO1), BMP inhibition (NOGGIN), EGF (notably lacking from developed human TO medium), activin receptor-like kinase 5 (ALK5) inhibition (A83-01) as well as N-acetylcysteine, nicotinamide and fibroblast growth factor 10 (FGF10).…”

Section: Main Textmentioning

confidence: 99%

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From Pluripotent Stem Cells to Organoids and Bioprinting: Recent Advances in Dental Epithelium and Ameloblast Models to Study Tooth Biology and Regeneration

Hermans,

Hasevoets,

Vankelecom

et al. 2024

Stem Cell Rev and Rep

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Ameloblasts are the specialized dental epithelial cell type responsible for enamel formation. Following completion of enamel development in humans, ameloblasts are lost and biological repair or regeneration of enamel is not possible. In the past, in vitro models to study dental epithelium and ameloblast biology were limited to freshly isolated primary cells or immortalized cell lines, both with limited translational potential. In recent years, large strides have been made with the development of induced pluripotent stem cell and organoid models of this essential dental lineage – both enabling modeling of human dental epithelium. Upon induction with several different signaling factors (such as transforming growth factor and bone morphogenetic proteins) these models display elevated expression of ameloblast markers and enamel matrix proteins. The advent of 3D bioprinting, and its potential combination with these advanced cellular tools, is poised to revolutionize the field – and its potential for tissue engineering, regenerative and personalized medicine. As the advancements in these technologies are rapidly evolving, we evaluate the current state-of-the-art regarding in vitro cell culture models of dental epithelium and ameloblast lineage with a particular focus toward their applicability for translational tissue engineering and regenerative/personalized medicine. Graphical Abstract Future perspectives for in vitro modeling of dental epithelium and ameloblasts. Development of iPSC and organoid models that can reliably generate dental epithelium and ameloblast-like cells, together with advances in 3D bioprinting, provide promising tools for enamel research. Advanced models will provide new avenues for development of enamel repair/regeneration approaches, for testing of dental materials or drugs, studying host-pathogen and/or cell-cell interactions, in vitro modeling of enamel diseases (e.g. amelogenesis imperfecta) and developing novel insights in fundamental tooth biology (e.g. regulation of amelogenesis, lineage specification). Abbreviations: iPSC: induced pluripotent stem cells; TO: tooth organoids; DE: dental epithelium; AB: ameloblast.

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“…Regenerative dentistry is an emerging area of dental research, and in recent years, numerous articles have appeared dealing with tooth development, tissue regeneration, organoids, and stem cell differentiation [1][2][3][4][5][6][7][8]. A tooth consists of three mineralized tissues derived from three different tooth germ tissues: enamel organ, dental papilla, and dental follicle.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Current Studies to Elucidate Processes in Dental Follicle Cells Driving Osteogenic Differentiation

Morsczeck,

De Pellegrin,

Reck

et al. 2023

Biomedicines

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When research on osteogenic differentiation in dental follicle cells (DFCs) began, projects focused on bone morphogenetic protein (BMP) signaling. The BMP pathway induces the transcription factor DLX3, whichh in turn induces the BMP signaling pathway via a positive feedback mechanism. However, this BMP2/DLX3 signaling pathway only seems to support the early phase of osteogenic differentiation, since simultaneous induction of BMP2 or DLX3 does not further promote differentiation. Recent data showed that inhibition of classical protein kinase C (PKCs) supports the mineralization of DFCs and that osteogenic differentiation is sensitive to changes in signaling pathways, such as protein kinase B (PKB), also known as AKT. Small changes in the lipidome seem to confirm the participation of AKT and PKC in osteogenic differentiation. In addition, metabolic processes, such as fatty acid biosynthesis, oxidative phosphorylation, or glycolysis, are essential for the osteogenic differentiation of DFCs. This review article attempts not only to bring the various factors into a coherent picture of osteogenic differentiation in DFCs, but also to relate them to recent developments in other types of osteogenic progenitor cells.

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Organoids from mouse molar and incisor as new tools to study tooth-specific biology and development

Cited by 5 publications

References 51 publications

Unraveling the hidden complexity: Exploring dental tissues through single-cell transcriptional profiling

Unraveling the hidden complexity: Exploring dental tissues through single-cell transcriptional profiling

From Pluripotent Stem Cells to Organoids and Bioprinting: Recent Advances in Dental Epithelium and Ameloblast Models to Study Tooth Biology and Regeneration

Evaluation of Current Studies to Elucidate Processes in Dental Follicle Cells Driving Osteogenic Differentiation

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