Organoid models in lung regeneration and cancer

Wang, Jianhai; Li, Xianglu; Chen, Huaiyong

doi:10.1016/j.canlet.2020.01.030

Cited by 45 publications

(37 citation statements)

References 66 publications

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“…Lung cancer is generally thought to originate from the malignant transformation of adult lung stem cells ( Wang et al., 2020 ; Zhang et al., 2015 ). LADC is believed to originate from alveolar type 2 (AT2) cells or their progenitors.…”

Section: Discussionmentioning

confidence: 99%

Human Lung Adenocarcinoma-Derived Organoid Models for Drug Screening

et al. 2020

iScience

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Summary Lung cancer is an extremely heterogeneous disease, and its treatment remains one of the most challenging tasks in medicine. Few existing laboratory lung cancer models can faithfully recapitulate the diversity of the disease and predict therapy response. Here, we establish 12 patient-derived organoids from the most common lung cancer subtype, lung adenocarcinoma (LADC). Extensive gene and histopathology profiling show that the tumor organoids retain the histological architectures, genomic landscapes, and gene expression profiles of their parental tumors. Patient-derived lung cancer organoids are amenable for biomarker identification and high-throughput drug screening in vitro . This study should enable the generation of patient-derived lung cancer organoid lines, which can be used to further the understanding of lung cancer pathophysiology and to assess drug response in personalized medicine.

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Section: Discussionmentioning

confidence: 99%

Human Lung Adenocarcinoma-Derived Organoid Models for Drug Screening

et al. 2020

iScience

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“…They can recapitulate the structures and some functions of specific organs. Currently, alveolar and airway organoids are actively used in various areas of lung research, including lung regeneration, lung cancer, and infectious lung diseases, including coronavirus disease-19 (COVID-19) [ 24 , 26 , 27 ]. Interestingly, several novel studies have suggested that YAP plays a vital role in the autophagy of lung cancer and the formation of lung organoids [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

New Insights into the Clinical Implications of Yes-Associated Protein in Lung Cancer: Roles in Drug Resistance, Tumor Immunity, Autophagy, and Organoid Development

Yoo

Park

Kim

et al. 2021

Cancers

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Despite significant innovations in lung cancer treatment, such as targeted therapy and immunotherapy, lung cancer is still the principal cause of cancer-associated death. Novel strategies to overcome drug resistance and inhibit metastasis in cancer are urgently needed. The Hippo pathway and its effector, Yes-associated protein (YAP), play crucial roles in lung development and alveolar differentiation. YAP is known to mediate mechanotransduction, an important process in lung homeostasis and fibrosis. In lung cancer, YAP promotes metastasis and confers resistance against chemotherapeutic drugs and targeted agents. Recent studies revealed that YAP directly controls the expression of programmed death-ligand 1 (PD-L1) and modulates the tumor microenvironment (TME). YAP not only has a profound relationship with autophagy in lung cancer but also controls alveolar differentiation, and is responsible for tubular structure formation in lung organoids. In this review, we discuss the various roles and clinical implications of YAP in lung cancer and propose that targeting YAP can be a promising strategy for treating lung cancer.

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“…Since a complete cancer investigation using experimental animals is not possible due to the lack of representability of the substantial phenotypic and genetic heterogeneity among human patients, lung organoids obtained from stem cells / progenitors derived from regions of the pulmonary epithelium can be used for oncological pharmacological intervention testing and also for regeneration. [107] In general, most researches using cancerous 3D cultures aim at investigating patient-specific anticancer treatments, as well as prognostic forethought. In summary, several recent publications point that the in vitro cancer barrier is being transposed with the reconstruction of diversified tissue tumors on a plate, such as mammary gland, lungs, ovaries, and several others.…”

Section: Cancerous Cellsmentioning

confidence: 99%

Historical evolution of spheroids and organoids, and possibilities of use in life sciences and medicine

Sakalem

Síbio

Costa

et al. 2021

Biotechnology Journal

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Background: An impressive percentage of biomedical advances were achieved through animal research and cell culture investigations. For drug testing and disease researches, both animal models and preclinical trials with cell cultures are extremely important, but present some limitations, such as ethical concern and inability of representing complex tissues and organs. 3D cell cultures arise providing a more realistic in vitro representation of tissues and organs. Environment and cell type in 3D cultures can represent in vivo conditions and thus provide accurate data on cell-to-cell interactions, and cultivation techniques are based on a scaffold, usually hydrogel or another polymeric material, or without scaffold, such as suspended microplates, magnetic levitation, and microplates for spheroids with ultra-low fixation coating. Purpose and scope:This review aims at presenting an updated summary of the most common 3D cell culture models available, as well as a historical background of their establishment and possible applications. Summary:Even though 3D culturing is incapable of replacing other current research types, they will continue to substitute some unnecessary animal experimentation, as well as complement monolayer cultures. Conclusion:In this aspect, 3D culture emerges as a valuable alternative to the investigation of functional, biochemical, and molecular aspects of human pathologies.

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Organoid models in lung regeneration and cancer

Cited by 45 publications

References 66 publications

Human Lung Adenocarcinoma-Derived Organoid Models for Drug Screening

Human Lung Adenocarcinoma-Derived Organoid Models for Drug Screening

New Insights into the Clinical Implications of Yes-Associated Protein in Lung Cancer: Roles in Drug Resistance, Tumor Immunity, Autophagy, and Organoid Development

Historical evolution of spheroids and organoids, and possibilities of use in life sciences and medicine

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