Organoid Models for Precision Cancer Immunotherapy

Sun, Caiping; Lan, Huanrong; Fang, Xingliang; Yang, Xiaoyun; Jin, Ketao

doi:10.3389/fimmu.2022.770465

Cited by 26 publications

(29 citation statements)

References 98 publications

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“…In comparison to PDX models, PDE, by maintaining the phenotype and TME of the individual tumor, may provide a realistic environment to assess the functional ex vivo response to drugs. Several studies highlighted the usefulness of PDE-based drug screening and their predictive value or association with clinical responses, confirming the usefulness of this system to prospectively assess individual patient response (7,13,58).…”

Section: Discussionmentioning

confidence: 79%

“…In particular, ex vivo 3D culture platforms, which maintain most of the features of tumor immune microenvironment (TIME) ( 5 ) and capture drug-mediated dynamic modulations, can elucidate molecular and immune-related mechanisms underlying clinical response or resistance to therapy ( 6 ). This approach could help gathering a comprehensive mechanistic overview and define predictive biomarkers of clinical outcome in patients treated with ICI ( 7 ). Here, we provide an overview of the relevance and potential implications of the tumor explant culture system in studying ICI responses.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

3D tumor explant as a novel platform to investigate therapeutic pathways and predictive biomarkers in cancer patients

Rodolfo

Huber²,

Cossa³

et al. 2022

Front. Immunol.

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Immunotherapy with immune checkpoint inhibitors can induce durable clinical responses in different human malignancies but the number of responding patients remains globally modest. The limited therapeutic efficacy of ICI depends on multiple factors, among which the immune suppressive features of the tumor microenvironment play a key role. For this reason, experimental models that enable dissection of the immune-hostile tumor milieu components are required to unravel how to overcome resistance and obtain full-fledged anti-tumor immunity. Recent evidence supports the usefulness of 3D ex vivo systems in retaining features of tumor microenvironment to elucidate molecular and immunologic mechanisms of response and resistance to immune checkpoint blockade. In this perspective article we discuss the recent advances in patient-derived 3D tumor models and their potential in support of treatment decision making in clinical setting. We will also share our experience with dynamic bioreactor tumor explant culture of samples from melanoma and sarcoma patients as a reliable and promising platform to unravel immune responses to immune checkpoint inhibitors.

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Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

3D tumor explant as a novel platform to investigate therapeutic pathways and predictive biomarkers in cancer patients

Rodolfo

Huber²,

Cossa³

et al. 2022

Front. Immunol.

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“…Assembloids exhibit an even greater level of complexity, as multiple cell types are used to recapture the multitude of tissues of which the target organ is built in vivo [60,61]. PDCO-derived organoids are investigated as potential clinical tools for the personalization of oncological treatment and response prediction [62][63][64]. The merger of 3D-bioprinting and organoid technologies both accelerates organoid formation and increases the potential organoid complexity [65].…”

Section: D-bioprinting Applications In Solid Tumor Microenvironment R...mentioning

confidence: 99%

Perspectives for 3D-Bioprinting in Modeling of Tumor Immune Evasion

Staros

Michalak

Rusinek

et al. 2022

Cancers

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In a living organism, cancer cells function in a specific microenvironment, where they exchange numerous physical and biochemical cues with other cells and the surrounding extracellular matrix (ECM). Immune evasion is a clinically relevant phenomenon, in which cancer cells are able to direct this interchange of signals against the immune effector cells and to generate an immunosuppressive environment favoring their own survival. A proper understanding of this phenomenon is substantial for generating more successful anticancer therapies. However, classical cell culture systems are unable to sufficiently recapture the dynamic nature and complexity of the tumor microenvironment (TME) to be of satisfactory use for comprehensive studies on mechanisms of tumor immune evasion. In turn, 3D-bioprinting is a rapidly evolving manufacture technique, in which it is possible to generate finely detailed structures comprised of multiple cell types and biomaterials serving as ECM-analogues. In this review, we focus on currently used 3D-bioprinting techniques, their applications in the TME research, and potential uses of 3D-bioprinting in modeling of tumor immune evasion and response to immunotherapies.

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“…This new platform can be used to understand tumor biology and identify potential biomarkers, therapeutics, and personalized medicine strategies for pancreatic tumors (Boj et al, 2016). Tumor organoids were used to identify the behavior of breast cancer cells in a healthy mammary environment or even the tumor response to new drugs (Mohan et al, 2021;C.-P. Sun et al, 2022). Regarding the female reproductive tract, its study has been a biological challenge.…”

Section: Use Of Organoids In Health Care and Researchmentioning

confidence: 99%

Organoids of the male reproductive system: Challenges, opportunities, and their potential use in fertility research

Patrício

Santiago

Mano

et al. 2022

WIREs Mechanisms of Disease

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Organoids are units of function of a given organ able to reproduce, in culture, a biological structure similar in architecture and function to its counterpart in vivo. Today, it is possible to develop an organoid from a fragment of tissue, a stem cell located in an adult organ, an embryonic stem cell, or an induced pluripotent stem cell. In the past decade, many organoids have been developed which mimic stomach, pancreas, liver and brain tissues, optic cups, among many others. Additionally, different male reproductive system organs have already been developed as organoids, including the prostate and testis. These 3D cultures may be of great importance for urological cancer research and have the potential to be used in fertility research for the study of spermatozoa production and maturation, germ cells–somatic cells interactions, and mechanisms of disease. They also provide an accurate preclinical pipeline for drug testing and discovery, as well as for the study of drug resistance. In this work, we revise the current knowledge on organoid technology and its use in healthcare and research, describe the male reproductive system organoids and other biomaterials already developed, and discuss their current application. Finally, we highlight the research gaps, challenges, and opportunities in the field and propose strategies to improve the use of organoids for the study of male infertility situations. This article is categorized under: Reproductive System Diseases > Stem Cells and Development Reproductive System Diseases > Biomedical Engineering

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Organoid Models for Precision Cancer Immunotherapy

Cited by 26 publications

References 98 publications

3D tumor explant as a novel platform to investigate therapeutic pathways and predictive biomarkers in cancer patients

3D tumor explant as a novel platform to investigate therapeutic pathways and predictive biomarkers in cancer patients

Perspectives for 3D-Bioprinting in Modeling of Tumor Immune Evasion

Organoids of the male reproductive system: Challenges, opportunities, and their potential use in fertility research

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