Organogenesis of Cucumis metuliferus plantlets under the effects of LEDs and silver nanoparticles

Lai, Chern Shun; Kho, Ying Han; Chew, Bee Lynn; Raja, Pandian Bothi; Subramaniam, Sreeramanan

doi:10.1016/j.sajb.2022.04.020

Cited by 5 publications

(5 citation statements)

References 38 publications

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“…Contrary to our result, the longest roots were recorded in plants of P. radiata and P. pseudostrobus exposed to red LEDs, [26,43]. Summarizing, different plant species respond differently, even when using the same light treatment, which may be due to the genotypic characteristics of the plants or the physiological state of the explants [30,65].…”

Section: Discussioncontrasting

confidence: 99%

Adult Trees Cryptomeria japonica (Thunb. ex L.f.) D. Don Micropropagation: Factors Involved in the Success of the Process

Rojas-Vargas

Montalbán²,

Moncaleán³

2023

Forests

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Cryptomeria japonica (Thunb. ex L.f.) D. Don is a commercial tree native to Japan and is one of the most important forest species in that country and the Azores (Portugal). Because of the quality of C. japonica timber, several genetic improvement programs have been performed. Recently, some studies focusing on C. japonica somatic embryogenesis have been carried out. However, in this species, this process uses immature seeds as initial explants, and for this reason, it is not possible to achieve the maximum genetic gain (100% genetic of the donor plant). Although some studies have been made applying organogenesis to this species, the success of the process in adult trees is low. For this reason, our main goal was to optimize the micropropagation method by using trees older than 30 years as a source of plant material. In this sense, in a first experiment, we studied the effect of different types of initial explants and three basal culture media on shoot induction; then, two sucrose concentrations and two light treatments (LEDs versus fluorescent lights) were tested for the improvement of rooting. In a second experiment, the effects of different plant growth regulators (6-benzylaminopurine, meta-topolin, and thidiazuron) on shoot induction and the subsequent phases of the organogenesis process were analyzed. The cultures produced the highest number of shoots when QL medium (Quoirin and Lepoivre, 1977) and long basal explants (>1.5 cm) were used; the shoots obtained produced a higher number of roots when they were grown under red LED lights. Moreover, root induction was significantly higher in shoots previously induced with meta-topolin.

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Section: Discussioncontrasting

confidence: 99%

Adult Trees Cryptomeria japonica (Thunb. ex L.f.) D. Don Micropropagation: Factors Involved in the Success of the Process

Rojas-Vargas

Montalbán²,

Moncaleán³

2023

Forests

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“…In Cucumis metuliferus, explants showed high root induction rates when exposed to white LEDs and in a mixed circuit of blue and red [ 56 ]. Furthermore, Cedrela odorata in vitro cultures grown under white LEDs presented a higher number of roots formed [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, Cedrela odorata in vitro cultures grown under white LEDs presented a higher number of roots formed [ 47 ]. In this regard, Lai et al [ 56 ] explained that one reason for this result might be that white light provides with a wider variety of minor lights, which are also required by plants for optimal growth.…”

Section: Discussionmentioning

confidence: 99%

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Rojas-Vargas

Castander-Olarieta²,

Nascimento³

et al. 2023

Plants

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Pinus. ponderosa (P. Lawson and C. Lawson) is a commercial tree and one of the most important forest species in North America. Ponderosa pine suffers hardship when going through vegetative propagation and, in some cases, 15–30 years are needed to achieve full reproductive capacity. Based on previous works on P. ponderosa regeneration through in vitro organogenesis and trying to improve the published protocols, our objective was to analyze the influence of different types of explants, basal culture media, cytokinins, auxins, and light treatments on the success of shoot multiplication and rooting phases. Whole zygotic embryos and 44 µΜ 6-benzyladenine showed the best results in terms of explants survival. For shoot organogenesis, whole zygotic embryos and half LP (LP medium, Quoirin and Lepoivre, 1977, modified by Aitken-Christie et al., 1988) macronutrients were selected. A significant positive interaction between whole zygotic embryos and half LP macronutrients was found for the percentage of explants forming shoots. Regarding the light treatments applied, a significantly higher percentage of shoots elongated enough to be rooted was detected in shoots growing under blue LED at a light intensity of 61.09 µmol m−2 s−1. However, the acclimatization percentage was higher in shoots previously cultivated under fluorescent light at a light intensity of 61.71 µmol m−2 s−1. Anatomical studies using light microscopy and scanning electron microscopy showed the light treatments promoted differences in anatomical aspects in in vitro shoots; needles of plantlets exposed to red and blue LEDs revealed less stomata compared with needles from plantlets exposed to fluorescent light.

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“…[28] Beside these applications, they are used to promote plant growth by decreasing the defoliation and the formation of biologically active substances, thereby producing multiple shoots for micropropagation. [30] Furthermore, AgNPs exhibit excellent conductivity, high surface area, mitigated threat of overpotential and quick electron communication features when used to modify electrode surfaces. [31] Depending on their size dependent SPR properties that lead to solution color change, AgNPs-based colorimetric sensors have been extensively used for the detection of various analytes such as DNA, metallic anions and cations, proteins and small compounds.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, AgNPs are widely used in the pharmaceutical industry for the prevention and treatment of burns and skin wounds in the form of creams and unguents [28] . Beside these applications, they are used to promote plant growth by decreasing the defoliation and the formation of biologically active substances, thereby producing multiple shoots for micropropagation [30] . Furthermore, AgNPs exhibit excellent conductivity, high surface area, mitigated threat of over‐potential and quick electron communication features when used to modify electrode surfaces [31] .…”

Section: Introductionmentioning

confidence: 99%

Colorimetric Sensor Based AgNPs for the Detection of Cyanide using UV‐Vis Spectrophotometry

Girgin,

Akbıyık,

Zaman

et al. 2023

ChemistrySelect

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Cyanide (CN−) is known to be a very harmful ion that may accumulate in the air, soil and water, and become a critical risk to organisms, even at trace levels. Hence, it is very critical to develop sensitive and accurate analytical methods for the determination of this anion. In this study, a highly sensitive and simple approach was developed for the quantitative determination of cyanide by UV‐Vis spectrophotometry with the aid of synthesized silver nanoparticles (AgNPs) solutions. AgNPs are promising probes frequently used in the determination of both organic and inorganic pollutants. The AgNPs were prepared by reducing silver nitrate (AgNO3) with sodium borohydride (NaBH4) in the presence of Triton X‐100 as a stabilizer. The UV‐Vis absorption spectra showed that the interaction between cyanide ions and AgNPs made significant decreases in the absorption peak of the surface plasmon resonance (SPR) band at 405 nm. The developed colorimetric sensor exhibited exceptional sensing towards cyanide ions with good linearity in the range of 0.5 and 3.5 mM. The limit of detection (LOD) and the limit of quantification (LOQ) were estimated as 0.09 and 0.29 mM, respectively. Moreover, the proposed colorimetric method was successfully applied to different water samples.

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Organogenesis of Cucumis metuliferus plantlets under the effects of LEDs and silver nanoparticles

Cited by 5 publications

References 38 publications

Adult Trees Cryptomeria japonica (Thunb. ex L.f.) D. Don Micropropagation: Factors Involved in the Success of the Process

Adult Trees Cryptomeria japonica (Thunb. ex L.f.) D. Don Micropropagation: Factors Involved in the Success of the Process

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Colorimetric Sensor Based AgNPs for the Detection of Cyanide using UV‐Vis Spectrophotometry

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