Organization of the Hox gene cluster of the silkworm, Bombyx mori: a split of the Hox cluster in a non-Drosophila insect

Yasukochi, Yuji; Ashakumary, Laksmikutty A.; Wu, Chun-Ta; Yoshido, Atsuo; Nohata, Junko; Mita, Kazuei; Sahara, Ken

doi:10.1007/s00427-004-0441-1

Cited by 49 publications

(62 citation statements)

References 38 publications

(37 reference statements)

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“…Coverage in LG6 is the highest, 47.3%, since chromosome walking has been performed in the region around the homeobox genes (Yasukochi et al 2004).…”

Section: Resultsmentioning

confidence: 99%

“…Fluorescence in situ hybridization of BAC clones: Chromosomes were prepared from p50 females according to the methods previously described (Sahara et al 1999;Yasukochi et al 2004). The BAC-FISH analysis involving BAC-DNA extraction, probe labeling, hybridization, washing, and digital image acquisition and processing was carried out according to Sahara et al (2003) and Yoshido et al (2005).…”

Section: Methodsmentioning

confidence: 99%

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A Second-Generation Integrated Map of the Silkworm Reveals Synteny and Conserved Gene Order Between Lepidopteran Insects

et al. 2006

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A second-generation linkage map was constructed for the silkworm, Bombyx mori, focusing on mapping Bombyx sequences appearing in public nucleotide databases and bacterial artificial chromosome (BAC) contigs. A total of 874 BAC contigs containing 5067 clones (22% of the library) were constructed by PCRbased screening with sequence-tagged sites (STSs) derived from whole-genome shotgun (WGS) sequences. A total of 523 BAC contigs, including 342 independent genes registered in public databases and 85 expressed sequence tags (ESTs), were placed onto the linkage map. We found significant synteny and conserved gene order between B. mori and a nymphalid butterfly, Heliconius melpomene, in four linkage groups (LGs), strongly suggesting that using B. mori as a reference for comparative genomics in Lepidotera is highly feasible.

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“…Coverage in LG6 is the highest, 47.3%, since chromosome walking has been performed in the region around the homeobox genes (Yasukochi et al 2004).…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A Second-Generation Integrated Map of the Silkworm Reveals Synteny and Conserved Gene Order Between Lepidopteran Insects

et al. 2006

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“…The honey bee Hox cluster has the characteristics expected of a canonical insect Hox gene cluster. First, similar to Tribolium (Brown et al 2002), the honey bee complex is not split in two as in Drosophila and Bombyx (Lewis et al 2003;Yasukochi et al 2004). Second, it contains all 10 of the expected Hox genes (Fig.…”

Section: The Hox Complexmentioning

confidence: 89%

Patterns of conservation and change in honey bee developmental genes

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Wilson²,

Sablan³

et al. 2006

Genome Res.

146

155

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The current insect genome sequencing projects provide an opportunity to extend studies of the evolution of developmental genes and pathways in insects. In this paper we examine the conservation and divergence of genes and developmental processes between Drosophila and the honey bee; two holometabolous insects whose lineages separated ∼300 million years ago, by comparing the presence or absence of 308 Drosophila developmental genes in the honey bee. Through examination of the presence or absence of genes involved in conserved pathways (cell signaling, axis formation, segmentation and homeobox transcription factors), we find that the vast majority of genes are conserved. Some genes involved in these processes are, however, missing in the honey bee. We have also examined the orthology of Drosophila genes involved in processes that differ between the honey bee and Drosophila. Many of these genes are preserved in the honey bee despite the process in which they act in Drosophila being different or absent in the honey bee. Many of the missing genes in both situations appear to have arisen recently in the Drosophila lineage, have single known functions in Drosophila, and act early in developmental pathways, while those that are preserved have pleiotropic functions. An evolutionary interpretation of these data is that either genes with multiple functions in a common ancestor are more likely to be preserved in both insect lineages, or genes that are preserved throughout evolution are more likely to co-opt additional functions.

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“…they maintain some degree of genomic organization) (Aboobaker and Blaxter, 2003 (Von Allmen et al, 1996;Negre and Ruis, 2007). Other non-dipteran insects show a type S cluster, as in the moth Bombix mori, although a breakpoint lies closer to the 'anterior extremity' of the gene series (Yasukochi et al, 2004). By contrast, some insect species have the full complement of Hox genes at a single locus.…”

Section: There Are Clusters and Clustersmentioning

confidence: 99%

The rise and fall of Hox gene clusters

2007

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Although all bilaterian animals have a related set of Hox genes, the genomic organization of this gene complement comes in different flavors. In some unrelated species, Hox genes are clustered; in others, they are not. This indicates that the bilaterian ancestor had a clustered Hox gene family and that, subsequently, this genomic organization was either maintained or lost. Remarkably, the tightest organization is found in vertebrates, raising the embarrassingly finalistic possibility that vertebrates have maintained best this ancestral configuration. Alternatively, could they have co-evolved with an increased `organization' of the Hox clusters, possibly linked to their genomic amplification, which would be at odds with our current perception of evolutionary mechanisms? When discussing the why's and how's of Hox gene clustering, we need to account for three points: the mechanisms of cluster evolution; the underlying biological constraints; and the developmental modes of the animals under consideration. By integrating these parameters, general conclusions emerge that can help solve the aforementioned dilemma. “See my son, here time becomes space” Gurnemanz, in Parsifal (R. Wagner)

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Organization of the Hox gene cluster of the silkworm, Bombyx mori: a split of the Hox cluster in a non-Drosophila insect

Cited by 49 publications

References 38 publications

A Second-Generation Integrated Map of the Silkworm Reveals Synteny and Conserved Gene Order Between Lepidopteran Insects

A Second-Generation Integrated Map of the Silkworm Reveals Synteny and Conserved Gene Order Between Lepidopteran Insects

Patterns of conservation and change in honey bee developmental genes

The rise and fall of Hox gene clusters

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