Organization of the cysts in bee (Hymenoptera, Apidae) testis: number of spermatozoa per cyst

Cruz-Landim, Carminda da

doi:10.1590/s0073-47212001000200025

Cited by 31 publications

(26 citation statements)

References 16 publications

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“…The cystic organization of spermatogonial lineage cells is also common for Hymenoptera ( Cruz-Landim, 2001). However, the number of spermatids per cyst varies among different species depending on the number of spermatogonial premeiotic divisions ( Oguma et al, 1987, Quagio-Grassioto and Lello, 1996, Cruz-Landim, 2001and Zama et al, 2007.…”

Section: Discussionmentioning

confidence: 98%

Morphology of the male reproductive system and spermiogenesis in Hypanthidium foveolatum (Alfken, 1930) (Hymenoptera: Apidae: Megachilinae)

Gracielle

Fiorillo

Lino‐Neto

et al. 2009

Micron

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The morphological aspects of male reproductive tract, spermiogenesis and spermatozoa are typical for each species and reflect its evolution, establishing a unique source of characters, which has been used to help solve phylogenetic problems. In Hypanthidium foveolatum the reproductive tract is composed of the testes comprising 28 testicular tubules, deferent ducts, seminal vesicles, accessory glands and an ejaculatory duct. The differentiation of spermatids occurs within cysts of up to 128 germ line cells each one. During the early spermatid phase, the nucleus resembles that of somatic cells. There follows a gradual chromatin condensation with an increase in nuclear electron density. In the spermatozoon, the nucleus contains heterogeneous chromatin with a loose appearance. The acrosome, shaped with the active participation of the Golgi complex, shows an electron-dense perforatorium involved by four electron-lucent acrosomal vesicle projections. The sperm tail presents an axoneme with a 9 + 9 + 2 microtubule pattern and two mitochondrial derivatives, which appear with different sizes. A dense crystalloid is formed initially in the mitochondrial matrix of the large derivative. The mitochondrial derivatives' differentiation occurs concomitantly with an axoneme outgrowth. The centriolar adjunct is observed near the axoneme, anterior to the smaller mithocondrial derivative and exhibits an approximately triangular shape in cross-sections. Microtubules were observed around the head region and flagellar components during spermiogenesis.

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Section: Discussionmentioning

confidence: 98%

Morphology of the male reproductive system and spermiogenesis in Hypanthidium foveolatum (Alfken, 1930) (Hymenoptera: Apidae: Megachilinae)

Gracielle

Fiorillo

Lino‐Neto

et al. 2009

Micron

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“…Research shows that for Diptera, the number of spermatids per bundle varies among different species depending on the number of spermatogonial premeiotic divisions (Oguma et al, 1987, Quagio-Grassiotto and Lello, 1996and Cruz-Landim, 2001). For Aedes aegypti (Culicidae), it was suggested by Owusu-Daaku et al (2007), that the maximum number of spermatozoa per cyst for this species is probably 512, being the result of nine divisions from the original spermatocyst mother cell (stem cell), according to what has been proposed by Virkki (1969).…”

Section: Discussionmentioning

confidence: 99%

Structure and ultrastructure of spermatozoa of Chrysomya megacephala (Diptera: Calliphoridae)

Name

Pujol-Luz

Báo

2010

Micron

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“…There is a seminal vesicle in each deferent duct, where the spermatozoa are stored until copulation and in each duct opens an accessory gland. However, there are structural variations in this pattern among groups of Hymenoptera, which have been used as sources of phylogenetic information (Cruz-Landim & Cruz-Hofl ing 1969, Gotwald & Burdette 1981, Wheeler & Krutzsch 1992, Duvoisin et al 1999, Baer & Schmid-Hempel 2000, Cruz-Landim 2001, Cruz-Landim & Dallacqua 2002, Baer 2003, Dallacqua & Cruz-Landim 2003, Tavares et al 2003, Ferreira et al 2004. Ferreira et al (2004) in a study on 51 bee species from six families (according to the Michener classification, 1965) divided the male reproductive systems into four types according to the number of testicular follicles and the portion of the deferent duct covered by the scrotal membrane.…”

Section: Palavras-chave: Histologia Testículo Vespa Esfeciformementioning

confidence: 99%

Morphology of male reproductive system in three species of Trypoxylon (Trypargilum) Richards (Hymenoptera: Crabronidae)

Moreira¹,

Araújo²,

Zama³

et al. 2008

Neotrop. entomol.

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-Variations in the adult male reproductive system among different groups of Hymenoptera offer characteristics that help studies on behavior and phylogenetics. The objective of this study was to describe the adult male reproductive system of three Trypoxylon (Trypargilum) species. For that, tissues were disseced, fi xed in 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.2 and postfi xed in 1% osmium tetroxide. The material was dehydratated and embedded for light and electron transmission microscopes. The species have similar reproductive systems, which are formed by a pair of testes, each one with three fusiforme follicles, from which emerges an efferent duct that later joins forming a deferent duct. The deferent duct opens into an ejaculatory duct. The fi rst half of the deferent duct is enlarged and differentiated in a region specialized in sperm storage, the seminal vesicle. The accessory gland fl ows in the post-vesicular region of the deferent duct. The testes and vesicles are both covered with a conjunctive capsule. Sexually mature individuals have all spermatogenesis stages in their follicles. Sperms are released from testes in bundles which are disorganized inside seminal vesicles.KEY WORDS: Histology, testes, spheciform wasp RESUMO -Variações no sistema reprodutor entre os diferentes grupos de Hymenoptera oferecem caracteres que auxiliam nos estudos de comportamento e fi logenia. O objetivo deste trabalho foi descrever o sistema reprodutor masculino de três espécies de Trypoxylon (Trypargilum). Para isso, os tecidos foram dissecados, fi xados em glutaraldeído 2,5% em tampão cacodilato de sódio 0,1 M, pH 7,2 e pós-fi xados em tetróxido de ósmio a 1%. O material foi desidratado e incluído para microscopias de luz e eletrônica de transmissão. As espécies possuem os sistemas reprodutores muito semelhantes, formados por um par de testículos, cada um com três folículos fusiformes, a partir dos quais emerge um ducto eferente que depois se juntam formando o ducto deferente. O ducto deferente termina no ducto ejaculatório. A primeira metade dos ductos deferentes é dilatada e diferenciada em uma região especializada no armazenamento de espermatozóides, a vesícula seminal. A glândula acessória desemboca na região pós-vesicular do ducto deferente. Testículos e vesículas seminais são envoltos por uma única cápsula conjuntiva. Indivíduos maduros sexualmente apresentam todos os estágios da espermatogênese em seus folículos. Os espermatozóides são liberados dos testículos em feixes, os quais estão desorganizados na vesícula seminal.

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Organization of the cysts in bee (Hymenoptera, Apidae) testis: number of spermatozoa per cyst

Cited by 31 publications

References 16 publications

Morphology of the male reproductive system and spermiogenesis in Hypanthidium foveolatum (Alfken, 1930) (Hymenoptera: Apidae: Megachilinae)

Morphology of the male reproductive system and spermiogenesis in Hypanthidium foveolatum (Alfken, 1930) (Hymenoptera: Apidae: Megachilinae)

Structure and ultrastructure of spermatozoa of Chrysomya megacephala (Diptera: Calliphoridae)

Morphology of male reproductive system in three species of Trypoxylon (Trypargilum) Richards (Hymenoptera: Crabronidae)

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