Organization of the bovine gene encoding the endothelial nitric oxide synthase

Venema, Richard C.; Nishida, Kozue; Alexander, R. Wayne; Harrison, David G.; Murphy, Thomas J.

doi:10.1016/0167-4781(94)90195-3

Cited by 104 publications

(48 citation statements)

References 30 publications

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“…Briefly, a primer set of 5′-GGCAGACCCCACCTTCTTGG-3′ and 5′-CCACAC TCTTCAAGTTGCCCAT-3′, designed based on the 5′-flanking regulatory region of bovine NOS3 gene at −1283 and + 22 (Genebank A# L27056) (Venema et al, 1994), was used for NOS3 promoter cloning. PCR was carried out using the Expand High Fidelity PCR System (Roche, Indianapolis, IN) in a reaction mixture (100 μl) of 0.25μg ovine genomic DNA polymerase under extracted from UAEC, 1.5mM MgCl 2 , 0.2mM dNTPs and 4.5units Taq DNA the conditions of 94°C for 2min, 30 cycles of denaturation (94°C, 15sec), annealing (60°C , 30sec), and extension (72°C, 2min).…”

Section: Cloning Of Sheep Nos3 Promoter and Reconstruction Of Nos3 Prmentioning

confidence: 99%

“…Sequence comparison indicated that the homology of sheep NOS3 promoter to that of bovine (Venema et al, 1994), human (Marsden et al, 1993), and mouse (Gnanapandithen et al, 1996) is 99.0%, 67.0%, and 56.1%, respectively. The putative regulatory sequences of the sheep NOS3 promoter (see Supplemental data) are diagramed in Fig.…”

Section: Cloning and Analysis Of Sheep Nos3 Promoter Sequencementioning

confidence: 99%

“…Similarly, the bovine and canine NOS3 promoters also have an AP-1 binding site (Schwemmer and Bassenge, 1999;Venema et al, 1994). We have recently isolated a 1305-bp sheep NOS3 promoter sequence.…”

Section: Introductionmentioning

confidence: 99%

“…We have recently isolated a 1305-bp sheep NOS3 promoter sequence. Sequence analysis revealed that sheep NOS3 promoter also possesses a consensus AP-1 binding site (5′-TGAGTCA-3′, −682 to −676) and many other cis-regulatory sequences similar to these of the human and bovine NOS3 promoters (Venema et al, 1994;Wariishi et al, 1995;Zhang et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional regulation of endothelial nitric oxide synthase expression in uterine artery endothelial cells by c-Jun/AP-1

Qian¹,

Mata‐Greenwood²,

Wu³

et al. 2007

Molecular and Cellular Endocrinology

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Despite extensive studies have shown that increased endothelial nitric oxide synthase (NOS3) expression in the uterine artery endothelial cells (UAEC) plays a key role in uterine vasodilatation, the molecular mechanism controlling NOS3 expression in UAEC is unknown. According to the sheep NOS3 promoter sequence isolated in our laboratory, we hypothesize that the activator protein-1 (AP-1) site in the proximal sheep NOS3 promoter (TGAGTCA, -682 to -676) is important for NOS3 expression. We developed a c-Jun adenoviral expression system to overexpress c-Jun protein into UAEC to investigate the effects of c-Jun/AP-1 on NOS3 expression. Basal levels of c-Jun protein and mRNA were detected in UAEC. C-Jun protein was overexpressed in a concentration and timedependent fashion in UAEC infected with sense c-Jun (S-c-Jun), but not sham and antisense c-Jun (A-c-Jun) adenoviruses. Infection with S-c-Jun adenovirus (25 MOI, multiplicity of infection) resulted in efficient c-Jun protein overexpression in UAEC up to 3 days. In S-c-Jun, but not sham and A-c-Jun adenovirus infected UAEC, NOS3 mRNA and protein levels were increased (P<0.05) compared to noninfected controls. Increased NOS3 expression was associated with increased total NOS activity. Transient transfections showed that c-Jun overexpression augmented the transactivation of the sheep NOS3 promoter-driven luciferase/reporter constructs with the AP-1 site but not of deletion constructs without the AP-1 site. When the AP-1 site was mutated, c-Jun failed to trans-activate the sheep NOS3 promoter. AP-1 DNA binding activity also increased in c-Jun overexpressed UAEC. Lastly, the pharmacological AP-1 activator phorbol myristate acetate increased AP-1 binding, trans-activated the wild-type but not the AP-1 mutant NOS3 promoter and dose-dependently stimulated UAEC NOS3 and c-Jun protein expression. Hence, our data show that c-Jun/AP-1 regulates NOS3 transcription involving the proximal AP-1 site in the 5′-regulatory region of the sheep NOS3 gene. Keywordsc-Jun/AP-1; endothelial nitric oxide synthase expression; uterine artery endothelial cells

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Section: Cloning Of Sheep Nos3 Promoter and Reconstruction Of Nos3 Prmentioning

confidence: 99%

Section: Cloning and Analysis Of Sheep Nos3 Promoter Sequencementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transcriptional regulation of endothelial nitric oxide synthase expression in uterine artery endothelial cells by c-Jun/AP-1

Qian¹,

Mata‐Greenwood²,

Wu³

et al. 2007

Molecular and Cellular Endocrinology

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“…Currently, the mechanisms or the significance behind these events are not clear. Recently, the presence of AP-l sites have been reported in both the human [35] and bovine ecNOS promoter [36]. Also, cyclic GMP has been implicated in the induction of the immediate early genes c-fos and junB and the formation of AP-1 complex [13,14].…”

mentioning

confidence: 99%

Up‐regulation of endothelial nitric oxide synthase expression by cyclic guanosine 3′,5′‐monophosphate

Ravichandran

Johns

1995

FEBS Letters

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In this study, the role of cyclic GMP, the end product of the NO-cyclic GMP signalling pathway, in the regulation of ecNOS was investigated. Bovine pulmonary endothelial cells were exposed to 8-bromo-cyclic GMP and its effect on NO production, ecNOS protein, and mRNA levels was analyzed. Endothelial cells on exposure to 8-bromo-cyclic GMP produced significantly increased amounts of NO, detected as increased cyclic GMP in cocultures with vascular smooth muscle cells both under basal conditions and with agonist stimulation. 8-Bromo-cyclic GMP significantly increased the ecNOS protein and mRNA levels as detected on Western and Northern blots respectively. This 8-bromo-cyclic GMP mediated increase of NO production, ecNOS protein and mRNA levels suggests that cyclic GMP up-regulates the expression of ecNOS. Thus, there may be an intercellular feedback mechanism involved at the molecular level in the expression of the NO-cyclic GMP signalling pathway in blood vessels.

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Inhibitors of endothelin

Webb

Meek

1997

Med. Res. Rev.

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Organization of the bovine gene encoding the endothelial nitric oxide synthase

Cited by 104 publications

References 30 publications

Transcriptional regulation of endothelial nitric oxide synthase expression in uterine artery endothelial cells by c-Jun/AP-1

Transcriptional regulation of endothelial nitric oxide synthase expression in uterine artery endothelial cells by c-Jun/AP-1

Up‐regulation of endothelial nitric oxide synthase expression by cyclic guanosine 3′,5′‐monophosphate

Inhibitors of endothelin

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