Organization of<i>Plasmodium falciparum genome</i>: 1. Evidence for a highly repeated DNA sequence

Guntaka, Ramareddy V.; Gowda, Siddarame; Rao, Arepalli S.; Green, Theodore J.

doi:10.1093/nar/13.6.1965

Cited by 14 publications

(4 citation statements)

References 25 publications

(28 reference statements)

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“…A more rapid approach for detection has been based on the use of DNA probes. A repetitive DNA sequence {rep 20) from P. falciparum has been described by several independent investigators (Franzen et al 1984;Guntaka et al 1985;Bhasin et al 1985;Aslund et al 1985;Oquendo et al 1986). It consists of multiple, imperfect repeats of 21 base pairs and as many as 13 copies of the repeat can occur in tandem (Oquendo et al 1986).…”

Section: Introductionmentioning

confidence: 99%

The use of biotin-labelled, synthetic DNA oligomers for the detection and identification of Plasmodium falciparum

Hughes

Hommel

Crampton³

1990

Parasitology

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An oligonucleotide mixture based on the 21 base pair repeat sequence of Plasmodium falciparum was covalently coupled to biotin and used as a probe to detect P. falciparum DNA. The limit of detection was 10 ng. This method was further developed as a fingerprint assay for parasite strain typing. After restriction enzyme digestion, blotting and hybridization, distinct banding patterns were obtained for the strains tested and these were reproducible. In addition, discrete differences were found between PLF-3 S+/S-strains which may implicate genetic reorganization in the switching mechanism which occurs when parasites are passed from an intact to a splenectomized animal.

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Section: Introductionmentioning

confidence: 99%

The use of biotin-labelled, synthetic DNA oligomers for the detection and identification of Plasmodium falciparum

Hughes

Hommel

Crampton³

1990

Parasitology

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“…In the subterminal chromosomal regions of most eukaryotic organisms studied to date, minisatellites have been found that consist of tandemly arranged repeat units of 21 bp to about 2 kb. For instance, subterminal minisatellites have been described in rye (Bedbrook et al 1980a), Plasmodium (,~s-lund et al 1985;Guntaka et al 1985;Oquendo et al 1986;Pace et al 1987), Dictyostelium (Emery and Weiner 1981), Saccharomyces , Drosophila (Bachmann et al 1990;Karpen and Spradling 1992;Levis et al 1993), Chironomus (Carmona et al 1985;Saiga and Edstr6m 1985), and man (Simmler et al 1987;Cheng et al 1989;Brown et al 1990;de Lange et al 1990;Rouyer et al 1990).…”

Section: Introductionmentioning

confidence: 99%

DNA organization and polymorphism of a wild-type Drosophila telomere region

et al. 1995

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Telomeres at the ends of linear chromosomes of eukaryotes protect the chromosome termini from degradation and fusion. While telomeric replication/elongation mechanisms have been studied extensively, the functions of subterminal sequences are less well understood. In general, subterminal regions can be quite polymorphic, varying in size from organism to organism, and differing among chromosomes within an organism. The subterminal regions of Drosophila melanogaster are not well characterized today, and it is not known which and how many different components they contain. Here we present the molecular characterization of DNA components and their organization in the subterminal region of the left arm of chromosome 2 of the Oregon RC wild-type strain of D. melanogaster, including a minisatellite with a 457bp repeat length. Two distinct polymorphic arrangements at 2L were found and analyzed, supporting the Drosophila telomere elongation model by retrotransposition. The high incidence of terminal chromosome deficiencies occurring in natural Drosophila populations is discussed in view of the telomere structure at 2L.

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“…Such repetitive DNA, however, is an excellent target for DNA-based diagnosis of infections caused by eucaryotic pathogens, because its abundance and rapid evolution allow sensitive and specific detection. Several plasmid clones have been isolated that may be suitable as oligonucleotide-based diagnostic probes for detection of Plasmodium falciparum (3,4,(7)(8)(9)16). Several of these clones have been sequenced and have been shown to contain a family of highly repetitive, somewhat degenerate 21-base-long DNA sequences (3,8,16).…”

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confidence: 99%

Comparison of genomic, plasmid, synthetic, and combined DNA probes for detecting Plasmodium falciparum DNA

McLaughlin¹,

Collins²,

Campbell³

1987

J Clin Microbiol

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Total genomic Plasmodiumfalciparum DNA, the plasmid clone pRepHind, and a 21-base-long synthetic DNA probe (PFR1), the sequence of which was derived from pRepHind, were hybridized with DNA from various species of the phylum Apicomplexa. The genomic probe hybridized with P. reichenowi and P. falciparum DNA and significantly cross-hybridized with DNA of ail the other Plasmodium species tested. The synthetic and plasmid probes hybridized to P. falciparum DNA and at reduced levels to P. reichenowi but did not hybridize to P. vivax, P. malariae, P. ovale, P. fragile, P. inui, P. knowlesi, Babesia bovis, B. microti, B. bigemina, Anopheles sp., Pan sp., Aotus sp., or human DNA. Southern blot analysis indicated that approximately 60 distinct restriction enzyme fragments from P.falciparum DNA were similarly detected by PFR1 and pRepHind. A method was developed by using a second brief hybridization with synthetic DNA to amplify signals from samples that were previously hybridized with plasmid-borne repetitive DNA. This amplification procedure was shown to allow the detection of 0.005% P. falciparum parasitemias from 10-,ul samples of blood from patients in Kenya.

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Organization ofPlasmodium falciparum genome: 1. Evidence for a highly repeated DNA sequence

Cited by 14 publications

References 25 publications

The use of biotin-labelled, synthetic DNA oligomers for the detection and identification of Plasmodium falciparum

The use of biotin-labelled, synthetic DNA oligomers for the detection and identification of Plasmodium falciparum

DNA organization and polymorphism of a wild-type Drosophila telomere region

Comparison of genomic, plasmid, synthetic, and combined DNA probes for detecting Plasmodium falciparum DNA

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