Organization and Transcription of Canine (CAC)n Sequences

Hershfield, Bennett; Inouye, Laura S.; Chader, Gerald J.; Ripps, Harris; Aguirre, G.D.

doi:10.1093/oxfordjournals.jhered.a111077

Cited by 7 publications

(3 citation statements)

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“…In contrast to minisatellite probes which have been used for DNA fingerprinting in different species including dogs (J effreys et al 1985; J effreys and M orton 1987; G eorges et al 1988), oligonucleotide probes offer the advantage that hybridization can be performed directly in the gel (P urrello and B alazs 1983; T sao et al 1983). The oligonucleotide probes (GT) 8 and (GTG) 5 have been described to yield polymorphic banding patterns in dogs (B uitkamp et al 1991; H ershfield et al 1991). However, the informativeness for identity and paternity testing was not investigated.…”

Section: Summary Of Dna Fingerprinting Results Using Different Oligmentioning

confidence: 99%

Oligonucleotide probes for DNA fingerprinting in dogs

Jung

Wilke

Geldermann

1994

J Animal Breeding Genetics

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Ten different oligonucleotide probes were evaluated for DNA fingerprinting in dogs. Seven probes are able to detect polymorphic bands. Probes (GT)(8) , (GTG)(5) and (GGAT)(4) are most informative for individual identification. The probabilities that two individuals from different breeds have the same DNA fingerprint pattern are 1.7 × 10(-7) , 5.5 × 10(-8) and 4.5 × 10(-6) , respectively. Using a combination of the three probes, paternity tests were performed with exclusion probabilities between 0.006% and 3%. ZUSAMMENFASSUNG: Oligonucleotid Sonden für DNA fingerprinting bei Hunden Zur Darstellung von DNA-Fingerprints beim Hund wurden zehn verschiedene Oligonukleotid-Sonden verglichen. Mit sieben Sonden konnten polymorphe Banden nachgewiesen werden. Die Sonden (GT)(8) , (GTG)(5) und (GGAT)(4) besaßen die größte Informativität für den Indentitätsnachweis. Die Wahrscheinlichkeit, daß zwei Individuen dieselben Fingerprint-Muster aufweisen, liegt bei 1,7 × 10(-7) , 5,5 × 10(-8) bzw. 4,5 × 10(-6) . Bei Verwendung einer Kombination der drei Sonden wurden Vaterschaftskontrollen mit Ausschlußwahrscheinlichkeiten zwischen 0,006% und 3 % erreicht.

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Section: Summary Of Dna Fingerprinting Results Using Different Oligmentioning

confidence: 99%

Oligonucleotide probes for DNA fingerprinting in dogs

Jung

Wilke

Geldermann

1994

J Animal Breeding Genetics

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“…However, because DNA fingerprinting patterns are complex and individual-specific, their forensic use has been confined to determining whether a particular individual within a particular species contributed biological material to a sample. We have recently studied the usefulness of a (CAC), repeat for DNA fingerprinting of canine pedigrees [14]. As an outgrowth of that project, we became interested in comparing the pattern of interspersion of (CAC), repeats in the dog genome against other vertebrates.…”

Section: Introductionmentioning

confidence: 99%

A polymerase chain reaction‐based method for the identification of DNA samples from common vertebrate species

1994

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Polymerase chain reaction (PCR) amplification of vertebrate genomic DNAs using a (CAC)n primer was found to generate species-specific patterns which are resolvable by agarose gel electrophoresis. Of the thirteen species tested (trout, frog, chicken, mouse, rat, cow, dog, African green monkey, chimpanzee, orangutan, gorilla, rhesus macaque, and human), all species showed discrete amplification products ranging in size from 1.0 to 3.2 kbp although trout and frogs had only weak (CAC)n amplification products. Most species had three to six bands except rodents, who had eight bands, and cows, who had a single band at 2.2 kbp. Importantly, within a single species, different unrelated individuals had highly similar amplification band patterns although the relative intensities of the bands varied. This held true even when different unrelated humans of different racial backgrounds were tested. We conclude that this technique is potentially useful for identifying which vertebrate species contributed DNA to a given biological sample (e.g., bloodstains, semen, hair) and that only a very small amount of sample is necessary for the analysis.

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“…Previous work on genetic polymorphism in dogs has been concentrated on studying the canine major histocompatibility complex (Sarmiento & Storb 1988), DNA fingerprinting (Jeffreys & Morton 1987;Georges et aJ. 1988;Hershfield et al 1991), and plasma proteins (e.g. Juneja et al 1987;Arnold & Bouw 1989).…”

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confidence: 99%

Cloning of a polymorphic canine genetic marker which maps to human chromosome 9

1993

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Summary We describe the cloning of a novel canine polymorphic genetic marker which maps to human chromosome 9. The sequence is 2092 bp, 59% GC rich, and contains three GC boxes. Chemilumin‐escent probing of zooblots showed evolutionary conservation. Dogs have three Bam HI alleles: 2.3 kb, 2.1 kb and 1.7 kb. Allele frequencies in 17 unrelated dogs representing 13 breeds are presented. Polymorphism for the 1.7‐kb allele in beagles is common. The 2.1‐kb allele is probably the ancestral allele since it is the most common and is also noted in the Cape hunting dog. Interestingly, in more than 50 dogs tested to date, the 2.3‐kb allele has been found only in miniature and giant schnauzers. This points to a common origin for these two breeds.

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Organization and Transcription of Canine (CAC)n Sequences

Cited by 7 publications

References 0 publications

Oligonucleotide probes for DNA fingerprinting in dogs

Oligonucleotide probes for DNA fingerprinting in dogs

A polymerase chain reaction‐based method for the identification of DNA samples from common vertebrate species

Cloning of a polymorphic canine genetic marker which maps to human chromosome 9

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