Organization and Relative Content of Smooth Muscle Cells, Collagen and Elastic Fibers in the Corpus Cavernosum of Rat Penis

Pinheiro, Ana Costa; Costa, Waldemar S.; Cardoso, Luis E.M.; Sampaio, Francisco J.B.

doi:10.1016/s0022-5347(05)67110-8

Cited by 50 publications

(72 citation statements)

References 32 publications

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“…The alleatory histological areas were quantified using a M-42 test-grid system on the digitized fields on the screen of a color monitor. The stereological method has been described in detail elsewhere (11,12).…”

Section: Methodsmentioning

confidence: 99%

Stereological study of collagen and elastic system in the detrusor muscle of bladders from controls and patients with infravesical obstruction

2007

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Objective: Compare detrusor muscle of normal and patients with infravesical obstruction, quantifying the collagen and elastic system fibers. Materials and Methods:We studied samples taken from bladders of 10 patients whose ages ranged from 45 to 75 years (mean = 60 years), who underwent transvesical prostatectomy for treatment of BPH. Control material was composed of 10 vesical specimens, removed during autopsies performed in cadavers of accident victims, with ages between 18 and 35 years (mean = 26 years). Results:The results of collagen and elastic fibers quantification (volumetric density) demonstrated the following results in percentage (mean +/-standard deviation): collagen in BPH patients = 4.89 +/-2.64 and 2.32 +/-1.25 in controls (p < 0.0001), elastin in BPH patients = 10.63% +/-2.00 and 8.94% +/-1.19 in controls (p < 0.0001). Conclusion:We found that the components of connective tissue, collagen and elastic system fibers are increased in the detrusor muscle of patients with infravesical obstruction, when compared to controls.

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Section: Methodsmentioning

confidence: 99%

Stereological study of collagen and elastic system in the detrusor muscle of bladders from controls and patients with infravesical obstruction

2007

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“…These structures also give required rigidity to the penis when in a flaccid state (2,3). Several studies have reported functional and morphological changes that take place during the erection process in different animal models (4,5) and also in humans (6,7).…”

Section: Introductionmentioning

confidence: 99%

Sinusoidal Constriction and Vascular Hypertrophy in the Diabetes-Induced Rabbit Penis

et al. 2013

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Objective: To assess the morphological changes of penile vascular structures and the corpus cavernosum area in alloxan-induced diabetic rabbits. Materials and Methods: Twenty male rabbits (2 months old) were divided into two groups with 10 rabbits each, the control group (CG) and the diabetic group (DG). The animals from DG received an intravenous injection of alloxan (100mg/kg) to induce the diabetes. Ten weeks after the induction of diabetes, all animals were euthanized. Two fragments of the penile shaft were harvested and samples were processed and paraffin embedded. Sections (5µm) were cut and stained for histological and immunohistochemical markers. Results: Nuclear protrusion toward the lumen, and cytoplasmic vacuolization were observed in the tunica intima of the dorsal artery of the penis in DG. The thicknesses of the tunica media increased significantly in DG (p = 0.0350). It was also observed a significant increase in the area of the tunica media (p = 0.0179). There was no significant change in smooth muscle cell density in the tunica media of the dorsal artery of the penis (p = 0.0855). The collagen fiber pattern of the tunica adventitia of the dorsal artery of the penis was different between the control and diabetic groups. There was a significant decrease in the area occupied by the cavernous sinuses in DG (p = 0.0013). Conclusion: Alloxan-induced diabetes mellitus in rabbits promotes important changes in penile vascular structures, thereby decreasing blood supply and affecting penile hemodynamics, leading to erectile dysfunction.

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“…In our previous investigation on the rabbit penis, we showed that the CC of young adult animals has a high concentration of elastic fibers 3 , which is greater than the values that have been reported for humans 14 and young adult rats 16 . This implies that, in rabbits, these fibrillar proteins play a particularly prominent role during erection and detumescence.…”

Section: Introductionmentioning

confidence: 55%

“…The Weigert-oxone technique as used herein stains all types of elastic fibers irrespective of the amount of elastin 9 . The specificity of the staining was further confirmed by immunolabeling tissue sections with an anti-elastin antibody (product code ab21610, polyclonal, Abcam, Cambridge, USA), as previously described 16 .…”

Section: Tissue Samples and Histological Techniquesmentioning

confidence: 99%

“…The distribution and structural features of elastic fibers in penile tissues have been investigated in humans [11][12][13][14][15] as well as in laboratory and food animals 3,16,17 . We have previously studied the concentration and distribution of elastic fibers in different regions of the rabbit penis, and the results indicated a close relationship with the known functions of penile tissues 3 .…”

Section: Introductionmentioning

confidence: 99%

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Age-related changes in the concentration of elastic fibers in different regions of the rabbit penis

Figueiredo

Costa

Chagas³

et al. 2013

Acta Cir. Bras.

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PURPOSE:To investigate whether elastic fiber content in the corpus cavernosum (CC), corpus spongiosum (CS) and tunica albuginea (TA) of the rabbit penis undergoes modifications with age. METHODS:Rabbits were sacrificed, in groups of ten animals each, at 30, 120, 240, and 730 days of age. Histological sections were obtained from the penile middle shaft and were stained with Weigert's resorsin fuchsin. The content of elastic fibers was determined using stereological methods, and was expressed as volume fraction. RESULTS:At 730 days of age, elastic fiber content was increased by 54% (p<0.004), 78% (p<0.004), and 87% (p<0.004) in the TA, CC, and CS, respectively, compared with animals aged 30 days. After 30 days of age, the concentration gradually and significantly increased until 240 days of age. In 730-day old animals, the concentration, compared with the previous age group, was unchanged in the CC and decreased by 20% (p<0.004) in the TA. CONCLUSIONS:Elastic fiber contents in the rabbit penis correlate with properties of penile tissues. Although after one month of age there is a gradual increase in these concentrations, in two-year old animals this trend is interrupted, which suggests that this could be an early alteration due to senescence.

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Organization and Relative Content of Smooth Muscle Cells, Collagen and Elastic Fibers in the Corpus Cavernosum of Rat Penis

Cited by 50 publications

References 32 publications

Stereological study of collagen and elastic system in the detrusor muscle of bladders from controls and patients with infravesical obstruction

Stereological study of collagen and elastic system in the detrusor muscle of bladders from controls and patients with infravesical obstruction

Sinusoidal Constriction and Vascular Hypertrophy in the Diabetes-Induced Rabbit Penis

Age-related changes in the concentration of elastic fibers in different regions of the rabbit penis

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