Organism and molecular-level responses of superoxide dismutase interaction with 2-pentanone

Sun, Kailun; Li, Meifei; Song, Yan; Tang, Jingchun; Liu, Rutao

doi:10.1016/j.chemosphere.2021.131707

Cited by 12 publications

(5 citation statements)

References 62 publications

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“…To test this hypothesis, we measured MDA levels, the end product of lipid peroxidation. 56 As expected, UV-transformed MoS 2 nanosheets induced significant lipid peroxidation (50.4−69.5%, p < 0.05; Figure 4C) in a dose-dependent fashion. The significant positive correlation between DG and MDA (R = 0.485, p = 0.0162; Figure 1M) further indicates that UTMoS 2 -induced lipid peroxidation was closely related to glyceride hypermetabolism.…”

Section: ■ Results and Discussionsupporting

confidence: 76%

“…67 Loss of earthworm epithelial integrity may lead to a higher exposure risk for the subepidermal muscle and intestinal tract. 56 This hypothesis was confirmed by the high-concentration groups. UTMoS 2 at 100 mg Mo/L resulted in more significant damage to the earthworm epithelium (Figure 5G 1 , red arrows).…”

Section: ■ Results and Discussionmentioning

confidence: 64%

“…Given the observed lipid dysregulation, we speculate that hypermetabolized liposomes, particularly polyunsaturated fatty acids, could be rapidly and extensively peroxidized by reactive oxygen species (ROS). To test this hypothesis, we measured MDA levels, the end product of lipid peroxidation . As expected, UV-transformed MoS 2 nanosheets induced significant lipid peroxidation (50.4–69.5%, p < 0.05; Figure C) in a dose-dependent fashion.…”

Section: Resultsmentioning

confidence: 95%

“…The earthworm epithelium is the primary barrier against environmental stress . Loss of earthworm epithelial integrity may lead to a higher exposure risk for the subepidermal muscle and intestinal tract . This hypothesis was confirmed by the high-concentration groups.…”

Section: Resultsmentioning

confidence: 99%

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Coupled Lipidomics and Digital Pathology as an Effective Strategy to Identify Novel Adverse Outcome Pathways in Eisenia fetida Exposed to MoS₂ Nanosheets and Ionic Mo

Sun

White

Qiu

et al. 2023

Environ. Sci. Technol.

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Molybdenum disulfide (MoS2) nanosheets are increasingly applied in several fields, but effective and accurate strategies to fully characterize potential risks to soil ecosystems are lacking. We introduce a coelomocyte-based in vivo exposure strategy to identify novel adverse outcome pathways (AOPs) and molecular endpoints from nontransformed (NTMoS2) and ultraviolet-transformed (UTMoS2) MoS2 nanosheets (10 and 100 mg Mo/L) on the earthworm Eisenia fetida using nontargeted lipidomics integrated with transcriptomics. Machine learning-based digital pathology analysis coupled with phenotypic monitoring was further used to establish the correlation between lipid profiling and whole organism effects. As an ionic control, Na2MoO4 exposure significantly reduced (61.2–79.5%) the cellular contents of membrane-associated lipids (glycerophospholipids) in earthworm coelomocytes. Downregulation of the unsaturated fatty acid synthesis pathway and leakage of lactate dehydrogenase (LDH) verified the Na2MoO4-induced membrane stress. Compared to conventional molybdate, NTMoS2 inhibited genes related to transmembrane transport and caused the differential upregulation of phospholipid content. Unlike NTMoS2, UTMoS2 specifically upregulated the glyceride metabolism (10.3–179%) and lipid peroxidation degree (50.4–69.4%). Consequently, lipolytic pathways were activated to compensate for the potential energy deprivation. With pathology image quantification, we report that UTMoS2 caused more severe epithelial damage and intestinal steatosis than NTMoS2, which is attributed to the edge effect and higher Mo release upon UV irradiation. Our results reveal differential AOPs involving soil sentinel organisms exposed to different Mo forms, demonstrating the potential of liposome analysis to identify novel AOPs and furthermore accurate soil risk assessment strategies for emerging contaminants.

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Section: ■ Results and Discussionsupporting

confidence: 76%

Section: ■ Results and Discussionmentioning

confidence: 64%

Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Coupled Lipidomics and Digital Pathology as an Effective Strategy to Identify Novel Adverse Outcome Pathways in Eisenia fetida Exposed to MoS₂ Nanosheets and Ionic Mo

Sun

White

Qiu

et al. 2023

Environ. Sci. Technol.

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“…A one-way analysis of variance (ANOVA) and Duncan's multiple tests were used for data analysis in gene expression and relative activity. The data for this study were processed by Graphpad Prism software (Version 9.0.2, San Diego, USA) [35].…”

Section: Discussionmentioning

confidence: 99%

An extracellular Cu/Zn superoxide dismutase from Neocaridina denticulata sinensis: cDNA cloning, mRNA expression and characterizations of recombinant protein

Feng¹,

Gao²,

Kong³

et al. 2022

Fish & Shellfish Immunology

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Behavioral, histopathological, genetic, and organism-wide responses to phenanthrene-induced oxidative stress in Eisenia fetida earthworms in natural soil microcosms

Yu²,

Shi

et al. 2022

Environ Sci Pollut Res

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Organism and molecular-level responses of superoxide dismutase interaction with 2-pentanone

Cited by 12 publications

References 62 publications

Coupled Lipidomics and Digital Pathology as an Effective Strategy to Identify Novel Adverse Outcome Pathways in Eisenia fetida Exposed to MoS₂ Nanosheets and Ionic Mo

Coupled Lipidomics and Digital Pathology as an Effective Strategy to Identify Novel Adverse Outcome Pathways in Eisenia fetida Exposed to MoS₂ Nanosheets and Ionic Mo

An extracellular Cu/Zn superoxide dismutase from Neocaridina denticulata sinensis: cDNA cloning, mRNA expression and characterizations of recombinant protein

Behavioral, histopathological, genetic, and organism-wide responses to phenanthrene-induced oxidative stress in Eisenia fetida earthworms in natural soil microcosms

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Organism and molecular-level responses of superoxide dismutase interaction with 2-pentanone

Cited by 12 publications

References 62 publications

Coupled Lipidomics and Digital Pathology as an Effective Strategy to Identify Novel Adverse Outcome Pathways in Eisenia fetida Exposed to MoS2 Nanosheets and Ionic Mo

Coupled Lipidomics and Digital Pathology as an Effective Strategy to Identify Novel Adverse Outcome Pathways in Eisenia fetida Exposed to MoS2 Nanosheets and Ionic Mo

An extracellular Cu/Zn superoxide dismutase from Neocaridina denticulata sinensis: cDNA cloning, mRNA expression and characterizations of recombinant protein

Behavioral, histopathological, genetic, and organism-wide responses to phenanthrene-induced oxidative stress in Eisenia fetida earthworms in natural soil microcosms

Contact Info

Product

Resources

About

Coupled Lipidomics and Digital Pathology as an Effective Strategy to Identify Novel Adverse Outcome Pathways in Eisenia fetida Exposed to MoS₂ Nanosheets and Ionic Mo

Coupled Lipidomics and Digital Pathology as an Effective Strategy to Identify Novel Adverse Outcome Pathways in Eisenia fetida Exposed to MoS₂ Nanosheets and Ionic Mo