Organisation and sequences at the 5′ end of a cloned complete ovalbumin gene

Gannon, Frank; O’Hare, Kevin; Perrin, F.; LePennec, J. P.; Benoist, C; Cochet, Madeleine; Breathnach, Richard; Royal, André; Garapin, Axel-Claude; Cami, B.; Chambon, Pierre

doi:10.1038/278428a0

Cited by 341 publications

(134 citation statements)

References 34 publications

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“…8 (a), and no sequences consistent with the consensus 'Hogness box' (Gannon et al, 1979) are present. The hexanucleotide AAUAAA has been found in close proximity to the 3' termini of the majority of polyadenylated eukaryotic mRNAs so far analysed (Benoist et al, 1980).…”

Section: Expression Of the Hs V-1 Joint Regionmentioning

confidence: 99%

Nucleotide Sequences of the Joint between the L and S Segments of Herpes Simplex Virus Types 1 and 2

Davison¹,

Wilkie²

1981

Journal of General Virology

224

201

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SUMMARYThe a sequence of herpes simplex virus (HSV) is present as a direct repeat at the genomic termini and also in inverted orientation at thc joint between the L and S segments. DNA sequences have been determined for the joint regions of the genomes of HSV-I and HSV-2, and relative to these sequences the genomic termini are in both cases located close to a short direct repeat of 17 to 21 base pairs (bp) at the b-a and a-c junctions. The HSV-I joint region contains three separate tandem direct reiterations of short sequences, (12, 16 and 17 bp in strain 17) and we conclude that size heterogeneity in'the a and c sequences is due to variable copy numbers of these repeated units. It is likely that a considerable part of the HSV-i joint region does not code for polypeptide.

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Section: Expression Of the Hs V-1 Joint Regionmentioning

confidence: 99%

Nucleotide Sequences of the Joint between the L and S Segments of Herpes Simplex Virus Types 1 and 2

Davison¹,

Wilkie²

1981

Journal of General Virology

224

201

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“…Overall, codon usage in the RAD3 gene would indicate this gene not to be a highly expressed one. (33,34) and is apparently required for proper positioning ofthe mRNA start at a specific site by RNA polymerase I (35)(36)(37)(38). In many yeast genes examined thus far, the distance between the mRNA start site and the TATA-like sequence is not as rigid as it is in higher eukaryotes, but varies considerably, being about 100 bp and 150 bp in the PYKI gene (39), 100 bp in the ADH1 gene (32), and 39 bp in the HIS4 gene (30).…”

Section: Sequence Of the Rad3 Gene And Proteinmentioning

confidence: 99%

The nucleotide sequence of theRAD3gene ofSaccharomyces cerevisiae: a potential adenine nucleotide binding amino acid sequence and a nonessential acidic carboxyl terminal region

et al. 1985

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The RAD3 gene of Saccharomyces cerevisiae is required for excision ofpyrimidine dimers and is essential for viability. We INTRODUCTIONIn the yeast Saccharomyces cerevisiae, excision of pyrimidine dimers or interstrand DNA crosslinks requires a large number of genes -RADI, RAD2, RAD3, RAD4, RAD1O, MMS19, RAD7, RAD14, RAD16, and RAD23 (1-12). A mutation in any of the first six of the ten genes listed results in highly defective incision of DNA containing pyrimidine dimers (13,14) or interstrand DNA crosslinks (8,11,12), while a mutation in RAD14, produces reduced incision proficiency compared with the Rad+ strain (9,13). The RAD3 gene has been cloned and partially characterized (15,16). Previously, we had localized the RAD3 gene to a DNA fragment of approximately 2.6 kb, and identified a 2.5 kb RAD3 mRNA and determined its direction of transcription (15). By integrating plasmids containing different internal fragments of the RAD3 gene in the yeast chromosomal RAD3 site, we, and others, deleted part of the RAD3 gene and found these deletions to be recessive lethal (15,17), indicating that RAD3 plays an essential role in cellular processes in addition to incision of damaged DNA. This finding is in contrast to the effect of radl, rad2, and radlO deletions or disruptions, which are viable (18-21) and suggests that the RAD3 gene plays a more complex role in vivo than do these other genes involved in incision ofpyrimidine dimer-containing DNA.In this paper, we have mapped the 5' end ofthe RAD3 mRNA and determined

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“…The test code indicator was calculated to be 1.04, indicating a high probability of it being proteincoding. The open reading frame is preceded by two TATATA sequences situated at positions 258 -263 and 324 -329, which are related to the TATA eukaryotic transcriptional signal [35]. The putative translational start has purine bases at positions -3 and + 4 relative to the A of the ATG codon, like many other eukaryotic translation start sites [36].…”

Section: Nucleotide Sequence Of the Cct Genementioning

confidence: 99%

Molecular cloning and characterization of the gene encoding cholinephosphate cytidylyltransferase in Saccharomyces cerevisiae

Tsukagoshi

Nikawa

Yamashita

1987

European Journal of Biochemistry

102

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1. The structural gene for cholinephosphate cytidylyltransferase (CCT) was isolated from a Sacchuromyces cerevisiae genomic library by means of complementation in a mutant of the yeast defective in the enzyme. The cloned DNA restored both the growth and cholinephosphate cytidylyltransferase activity of the mutant. Whereas the enzyme of the mutant was thermolabile, the enzyme produced by the transformant was indistinguishable in heat stability from that produced by the wild type.2. Strains carrying a multicopy recombinant plasmid overproduced cholinephosphate cytidylyltransferase. The overproduction of the enzyme brought about an increase in the synthesis of CDPcholine in the transformant, but there was no increase in the overall rate of phosphatidylcholine synthesis.3. The cloned DNA was subcloned into a 2.5-kb DNA fragment. The nucleotide sequence which contained CCT was determined by the dideoxy chain-termination method. The sequence contained an open reading frame capable of encoding a protein of 424 amino acid residues with a calculated relative molecular mass of 49 379.31. Northern blot analysis showed that this DNA segment is transcribed in yeast cells and the length of the transcript is consistent with the putative translation product.4. Hydropathy analysis according to Kyte and Doolittle indicated that the primary translation product contains extended hydrophilic stretches in its N-and C-terminal regions.5. The primary translation product contains a region showing local sequence homology with nucleotidyltransfer enzymes such as DNA polymerase (Escherichiu coli), CDPdiacylglycerol pyrophosphatase (E. coli), 3-deoxy-manno-octulosonate cytidylyltransferase (E. coli) and DNA ligase (T4 phage), suggesting that these five enzymes are evolutionarily related. Statistically significant sequence homology was also noted between the human c-fos gene product and the enzyme.Phosphatidylcholine is a major structural component of the cellular membranes of eukaryotes. In the yeast, Sacchuromyces cerevisiue, phosphatidylcholine comprises about 40% of the total phospholipids. Several lines of evidence indicate that phosphatidylcholine is absolutely required for yeast growth [l -31. S. cerevisiae possesses two pathways for the synthesis of phosphatidylcholine, the CDPcholine pathway and the phosphatidylethanolamine methylation pathway [4-61. Due to the feasibility of genetic manipulation of the yeast, the phosphatidylcholine-synthesizing system of S. cerevisiue has been the subject of extensive genetic studies. Mutants for all of the enzymes involved in the CDPcholine pathway and the phosphatidylethanolamine methylation pathway have been isolated [l, 2, 7-91. Characterization of these mutants has provided an insight into the genetic and

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Organisation and sequences at the 5′ end of a cloned complete ovalbumin gene

Cited by 341 publications

References 34 publications

Nucleotide Sequences of the Joint between the L and S Segments of Herpes Simplex Virus Types 1 and 2

Nucleotide Sequences of the Joint between the L and S Segments of Herpes Simplex Virus Types 1 and 2

The nucleotide sequence of theRAD3gene ofSaccharomyces cerevisiae: a potential adenine nucleotide binding amino acid sequence and a nonessential acidic carboxyl terminal region

Molecular cloning and characterization of the gene encoding cholinephosphate cytidylyltransferase in Saccharomyces cerevisiae

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