Organic Zinc and Copper Supplementation–Associated Changes in Gene Expression and Protein Profiles in Buck Spermatozoa

Mayasula, Venkata Krishnaiah; Arunachalam, A.; Selvaraju, Sellappan; Guvvala, Pushpa Rani; Ghosh, Jyotirmoy

doi:10.1007/s12011-021-02796-x

Cited by 6 publications

(5 citation statements)

References 36 publications

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“…Immunoblotting: This is the continuation of previously published work in cryobiology (Arunachalam et al 2018, Mayasula et al 2022, B. Sekar et al 2021. The 2D Gel analysed differentially expressed protein localized and identified through MALDI-TOF (methodology followed as discussed by Mayasula et al 2022). Briefly the 2-DE was carried out by re-suspending 60 μg protein in 125 μl rehydration buffer (0.2% biolyte ampholyte, 2 M thiourea, 50 mM dithiothreitol, 8 M urea, 2% CHAPS and added to immobilized pH gradient (IPG) strip (7 cm, 4-7 pI) (Biorad), followed by rehydration (19 h, 50 V at 20°C) (Protean IEF cell, Bio-Rad).…”

Section: Experimental Plan and 2 D Gel Electrophoresis Andmentioning

confidence: 54%

Section: Experimental Plan and 2 D Gel Electrophoresis Andmentioning

confidence: 88%

“…supplementation studies related to puberty onset in bucks, we identified that the sperms of T4 (Zn 60 mg on kg-DM) group semen had lost their viability at the end of equilibration (4 hr) and after cryopreservation (Arunachalam et al 2018, Mayasula et al 2022. Initially it was believed that this could be due to the deleterious effect of higher Zn on the spermatozoa plasma membrane or accelerated cold shock induced damage to spermatozoa.…”

Section: Experimental Plan and 2 D Gel Electrophoresis Andmentioning

confidence: 99%

“…The in vivo method, consists of supplementation of mineral, proteins, high energy diet to male animals and enhancement of semen characteristic prior to collection of semen samples (Arunachalam et al 2018;Hemalatha et al 2018;Narasimhaiah et al 2018). In the in vitro method, various types of semen additives are included during the dilution and equilibration period and prior to cryopreservation process (Dance et al 2016, Mayasula et al 2022. The obtained results and success of the in vivo and in vitro approaches are variable at a larger scale.…”

mentioning

confidence: 99%

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Novel Cold-Inducible RNA-Binding Protein (CIRBP) in goat spermatozoa: A possible cryo-stress reliever during the process of Cryopreservation

Arunachalam

BACKIALAKSHMI

SHARANYA

et al. 2022

Indian J of Anim Sci

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The great expectation of the dairy owners from the frozen semen industry is quality semen with high fertility. Many researchers concentrate in this area to produce frozen semen of high quality and fertility. However, the problem still exists to minimize or reduce the cryoinjury related damages that occur during the process of cryopreservation with the use of all kinds of semen additives. The success rate of the additives added semen is variable at a larger scale. Therefore, any new molecules which can effectively prevent or minimize the cryoinjury are warranted and it could be low cost substances. Our incidental finding and protein identification with molecular weight ≈ 19 kDa (cold-inducible RNA-binding protein CIRBP) in goat spermatozoa seems to be effective in minimizing cryoinjury. The identified CIRBP was confirmed via Western blotting. In future, further studies are needed to confirm the molecular mechanism of the CIRBP on the cryoinjury as semen additives, and application of this protein added semen in the field to improve the level of conception rate in cattle.

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Section: Experimental Plan and 2 D Gel Electrophoresis Andmentioning

confidence: 54%

Section: Experimental Plan and 2 D Gel Electrophoresis Andmentioning

confidence: 88%

Section: Experimental Plan and 2 D Gel Electrophoresis Andmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Novel Cold-Inducible RNA-Binding Protein (CIRBP) in goat spermatozoa: A possible cryo-stress reliever during the process of Cryopreservation

Arunachalam

BACKIALAKSHMI

SHARANYA

et al. 2022

Indian J of Anim Sci

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“…Nos últimos anos, o interesse na incorporação de minerais traço na forma orgânica para a alimentação animal tem crescido, diminuindo a ocorrência da adição na forma inorgânica. Este interesse tem sido estimulado devido os resultados de pesquisas que mostram a melhora no crescimento, na reprodução e na sanidade dos animais alimentados com minerais orgânicos (ULLAH et al, 2020;MAYASULA, 2022).…”

Section: Suplementação De Leveduras Com Zincounclassified

Produção de leveduras enriquecidas em zinco

Pinto

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A Deus, por todo Seu amor e misericórdia. À Virgem Maria, pelo seu colo de Mãe.Agradeço aos meus pais, Antonia e José, verdadeiros anjos, um na terra e outro no céu.Muito obrigada por todo o incentivo, apoio incondicional e por nunca me deixarem perder a fé.Ao meu irmão Edvando, que me ensinou que o amor é capaz de suportar todas as coisas.À minha família, pelo carinho e estímulo que me deram, especialmente aos meus padrinhos, Neto e Silani, que sempre foram exemplos e me apontaram o caminho.Ao Lenon Henrique, expresso minha profunda gratidão por ter sido um ponto de equilíbrio fundamental diante das dificuldades. Sua presença tornou esse caminho mais leve e significativo.À família Guerreiro Leandro, por sempre me apoiar e estar presente em todos os momentos.Agradeço ao meu orientador, Prof. Dr. Antonio Sampaio Baptista, pela oportunidade que me concedeu, por todos os conselhos, por compartilhar sua sabedoria, pela valiosa orientação e pela forma excelente com a qual guiou o meu aprendizado.À Layna Amorim, por ter me emprestado seu tempo, seu olhar minucioso que enriqueceu esse trabalho, pelos conselhos e por sua amizade especial.Agradeço às meninas do GTSBio, Gabriela Almeida, Tamires Faria, Vitória Franco e Chiara Vitti, que me acolheram muito bem e me apoiaram constantemente com as suas orações.Agradeço especialmente à Liandra Spironello, pelo companheirismo, amizade e contribuição nesse trabalho.

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In Vitro and In Vivo Studies on the Efficacy of Zinc-Oxide and Selenium Nanoparticle in Cryopreserved Goat (Capra hircus) Spermatozoa

Abedin

Baruah

et al. 2023

Biol Trace Elem Res

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Organic Zinc and Copper Supplementation–Associated Changes in Gene Expression and Protein Profiles in Buck Spermatozoa

Cited by 6 publications

References 36 publications

Novel Cold-Inducible RNA-Binding Protein (CIRBP) in goat spermatozoa: A possible cryo-stress reliever during the process of Cryopreservation

Novel Cold-Inducible RNA-Binding Protein (CIRBP) in goat spermatozoa: A possible cryo-stress reliever during the process of Cryopreservation

Produção de leveduras enriquecidas em zinco

In Vitro and In Vivo Studies on the Efficacy of Zinc-Oxide and Selenium Nanoparticle in Cryopreserved Goat (Capra hircus) Spermatozoa

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