Organic trace mineral supplementation enhances local and systemic innate immune responses and modulates oxidative stress in broiler chickens

Abstract

The effect of organic trace mineral supplementation on performance, intestinal morphology, immune organ weights (bursa of Fabricius and spleen), expression of innate immune response related genes, blood heterophils/lymphocytes ratio, chemical metabolic panel, natural antibodies (IgG), and oxidative stress of broiler chickens was studied. A total of 1,080 day-old male broilers were assigned to 1 of 3 dietary treatments, which included basal diet with Monensin (control), control diet supplemented with bacitracin methylene disalicylate (BMD), and BMD diet supplemented with organic trace minerals (OTM). No difference in feed conversion ratio was observed among treatments; ileum histomorphological analysis showed a lower crypt depth, higher villi height/crypt depth ratio, and lower villi width in the OTM treatment compared to control. Furthermore, OTM treatment resulted in higher uric acid and lower plasma malondehaldehyde (MDA), indicating lower oxidative stress. Gene expression analysis showed that OTM treatment resulted in up-regulations of TLR2 bin the ileum, and TLR2b, TLR4, and IL-12p35 in the bursa of Fabricius, and down-regulation of TLR2b and TLR4 in the cecal tonsils. In the spleen, OTM treatment resulted in up-regulation of IL-10. In conclusion, OTM supplementation to broiler diets may have beneficial effects on intestinal development, immune system status, and survival by improving ileum histomorphological parameters, modulation of Toll-like receptors and anti-inflammatory cytokines, and decreasing level of MDA, which in conjunction could enhance health status.

Advanced Chelate Technology Based Trace Minerals Improve Growth Performance, Mineral Digestibility, Tibia Characteristics, and Antioxidant Status in Broiler Chickens

“…The increase in blood uric acid concentration could be associated with the free radical scavenging capacity [27]. In a previous study where broiler chickens were fed diets that replaced inorganic TM with organic TM, the authors found a signi cantly higher serum uric acid level [1]. In part, these ndings could contribute to the possible reduction of tissue damage by organically complexed minerals.…”

“…In addition, the AID of Cu was higher (P < 0.05) in the CTM25 group relative to the CTM100 group. Table 5 Effect of experimental treatments on percent ileal absorption of minerals in broilers 1 Values in the same row with different superscripts were signi cantly different (P < 0.05).…”

“…In ITM and CTM50 groups, tibia Mn content was intermediate and signi cantly different from the other three diet groups. Table 6 Effect of experimental treatments on tibia quality and tibial mineral content in broilers 1 1 Values are means of 5 pens per treatment combination with 2 male broiler chickens per pen selected for tibia collection.…”

Effects of Azomite‐ and Sea Salt‐Supplemented Diets on Growth, Feed Conversion Efficiency, Survival, and Resistance against Edwardsiella ictaluri in Channel Catfish Fingerlings

“…Echeverry et al. () found that trace mineral supplementation enhanced local and systemic innate immune responses and modulated oxidative stress in broiler chickens. Furthermore, transcriptomic profiling indicated an enhanced immune response in Rainbow Trout that were fed selenium‐supplemented diets compared with those that were fed a basal diet (Pacitti et al.…”

“…In Vibrio alginolyticus-challenged shrimp, azomite supplementation increased survival rates (Tan et al 2014). Echeverry et al (2016) found that trace mineral supplementation enhanced local and systemic innate immune responses and modulated oxidative stress in broiler chickens. Furthermore, transcriptomic profiling indicated an enhanced immune response in Rainbow Trout that were fed selenium-supplemented diets compared with those that were fed a basal diet (Pacitti et al 2016), and the administration of mineral-enriched diets improved the disease resistance of Stinging Catfish Heteropneustes fossilis against Aeromonas hydrophila (Jawahar et al 2018).…”