Organic polyanions act as complexants of prion protein in soil

Polano, Maurizio; Anselmi, Claudio; Leita, L.; Negro, Alessandro; Nobili, Maria De

doi:10.1016/j.bbrc.2007.12.143

Cited by 38 publications

(47 citation statements)

References 24 publications

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“…40 In studies where the ability of SOM compounds to bind uninfectious recombinantly generated PrP was investigated, the affinity of SOM for recPrP was almost equivalent to that offered by the clay mineral surfaces. [17][18][19][20] In addition, pure HA not only exhibited the strongest affinity for recPrP but also increased (about 10×) the sorption capacity of kaolinite and Mte clays. 19 However, differences in the structure of infectious and non-infectious prion proteins require more judicious analyses of these results to estimate SOM-infectious prion interactions.…”

Section: Soil Organic Mattermentioning

confidence: 95%

“…[17][18][19][20] In addition, pure HA not only exhibited the strongest affinity for recPrP but also increased (about 10×) the sorption capacity of kaolinite and Mte clays. 19 However, differences in the structure of infectious and non-infectious prion proteins require more judicious analyses of these results to estimate SOM-infectious prion interactions. 40 The binding mechanism of SOM and its influence on prion infectivity is still poorly understood.…”

Section: Soil Organic Mattermentioning

confidence: 95%

“…[14][15][16]19,[26][27][28] Prions have a strong affinity for clay minerals commonly found in soil; PrP Sc binds clay minerals (montmorillonite, Mte) avidly in comparison with quartz sand, an interaction that considerably enhances prion infectivity.…”

Section: Prion Interactions With Different Soil Compoundsmentioning

confidence: 99%

“…usually vary with topography. To date, only a few soil characteristics have been investigated with respect to their influence on infectious and uninfected agents: some common soil minerals (montomorillonite, kaolinite and quartz), [14][15][16] organic polyanions and humic acids (as soil organic matter compounds), [17][18][19][20] and some metals and their oxides (Al 2 O 3 , SiO 2 , MnO). [21][22][23][24][25] The main focus of this review is the characterization of soil properties in the CWD-endemic region and its surrounding areas from Alberta to Manitoba to estimate their potential for binding prions and maintaining bioavailability.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Potential role of soil properties in the spread of CWD in western Canada

Kuznetsova

McKenzie

Banser

et al. 2014

Prion

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Section: Soil Organic Mattermentioning

confidence: 95%

Section: Soil Organic Mattermentioning

confidence: 95%

Section: Prion Interactions With Different Soil Compoundsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Potential role of soil properties in the spread of CWD in western Canada

Kuznetsova

McKenzie

Banser

et al. 2014

Prion

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“…Either the infective or the benign forms of prion proteins are able to strongly bind to mineral [8][9][10][11][12] and organic soil components [13][14][15]. PrP Sc can be displaced from environmental compartments to soil, but this does not necessarily decrease its infectivity [10,16].…”

Section: Introductionmentioning

confidence: 99%

Interaction of a Recombinant Prion Protein with Organo-Mineral Complexes as Assessed by FT-IR and CPMAS 13C NMR Analysis

Russo¹,

Gianfreda²,

Conte³

et al. 2010

TOMRJ

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Prion proteins are considered as the main agents for the Transmissible Spongiform Encephalopathies (TSE). The misfolded form, PrP Sc , which is also indicated as the etiological agent for TSE, exhibits high resistance to degradation in environmental processes. Soil contamination by prion proteins is a real environmental issue since contaminated soils can become potential reservoir and diffuser for TSE infectivity. In this work, the interaction of prion protein with organomineral complexes was studied by using a recombinant non pathogenic prion protein and a model soil system. This latter was represented by a soil manganese mineral coated with polymerized catechol. FT-IR spectra showed amide I and II signals which revealed protein involvement in catechol polymers coating manganese oxide surface. CPMAS 13 C-NMR was applied to follow the complexation of the protein to the soil-like system. All the signals were attributed to C-N in peptidic bonds, alkyl chains and methyl groups. The NMR spectrum of the prion protein interacting directly with birnessite revealed disappearance of signals due to the paramagnetic nature of manganese oxide or protein abiotic degradation, while the presence of organic matter strongly reduced the disappearance of prion protein signals.

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Location and stability of a recombinant ovine prion protein in synthetic humic-like mineral complexes

et al. 2011

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Location and stability of a recombinant prion protein (recPrP) and its interaction with humic-like complexes were investigated by low-temperature ashing (LTA), thermal gravimetric (TG), and scanning electron microscopy (SEM) analyses. Humic-like complexes were obtained by abiotic polymerization of catechol, one of the possible precursors of soil humic matter, through the catalysis of birnessite, a manganese oxide common in soil environment. The recPrP was immobilized in organomineral complexes via sorption or entrapment. Complexes were treated by LTA, allowing the controlled removal of organic matter layer by layer, from the external to the internal side, with minimal disturbance of mineral constituents. Thermal gravimetric and SEM analyses were performed on specimens before and after LTA treatment. Entrapped recPrP, compared with sorbed, resulted less easily accessible to LTA treatment and showed a higher thermal stability by TGA analyses. On the basis of these findings, we hypothesize that the processes leading to newly formed organic complexes can enhance prion stability in soil and thus influence the environmental diffusion of infectivity.

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Organic polyanions act as complexants of prion protein in soil

Cited by 38 publications

References 24 publications

Potential role of soil properties in the spread of CWD in western Canada

Potential role of soil properties in the spread of CWD in western Canada

Interaction of a Recombinant Prion Protein with Organo-Mineral Complexes as Assessed by FT-IR and CPMAS 13C NMR Analysis

Location and stability of a recombinant ovine prion protein in synthetic humic-like mineral complexes

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