Organic Peracids.

Swern, Daniel

doi:10.1021/cr60140a001

Cited by 243 publications

(114 citation statements)

References 118 publications

Supporting

Mentioning

108

Contrasting

Unclassified

Order By: Relevance

“…4 Despite their attractive synthesis potential, peroxycarboxylic acids are limited in applications due to hydrolysis in aqueous solutions 5 and their explosive "nature" in concentrated form. 6 In situ generation of peroxycarboxylic acids by hydrolysis of esters or amides in the presence of hydrogen peroxide is an alternative approach for producing active peroxycarboxylic acids when required in applications. 7 Peroxycarboxylic acid production employing hydrolases has been reported for proteases, 8,9 lipases, 10 and esterases, 11 which are mainly used in detergent formulations.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Assay for Directed Evolution of Perhydrolases

et al. 2012

View full text Add to dashboard Cite

Directed evolution offers opportunities to improve promiscuous activities of hydrolases in rounds of diversity generation and high-throughput screening. In this article, we developed and validated a screening platform to improve the perhydrolytic activity of proteases and likely other hydrolases (e.g., lipases or esterases). Key was the development of a highly sensitive fluorescent assay (sensitivity in the µM range) based on 3-carboxy-7-hydroxycoumarin (HCC) formation. HCC is released through an hypobromite-mediated oxidation of 7-(4′-aminophenoxy)-3-carboxycoumarin (APCC), which enables for the first time a continuous measurement of peroxycarboxylic acid formation with a standard deviation of 11% in microtiter plates with a wide pH range window (5-9). As example, subtilisin Carlsberg was subjected to site saturation mutagenesis at position G165, yielding a variant T58A/G165L/L216W with 5.4-fold increased k cat for perhydrolytic activity compared with wild type.

show abstract

Section: Introductionmentioning

confidence: 99%

Fluorescent Assay for Directed Evolution of Perhydrolases

et al. 2012

View full text Add to dashboard Cite

show abstract

“…No data are available on the -Japor pressure oi performic acid but from its behavior in fractional distillation it is known to be more volatile than formic acid. As for peracetic acid, it boils a t 10SO, 13 degrees lower than acetic acid, in spite of a higher n~olecular weight (22). For the salne reason it may be predicted that the entropj-of vaporization of the peracids is near the value of Trouton's constant, 21 cal.…”

mentioning

confidence: 95%

A Spectroscopic Study of Hydrogen Bonding in Performic and Peracetic Acids

Giguère¹,

Olmos²

1952

Can. J. Chem.

View full text Add to dashboard Cite

The infrared spectra of concentrated performic and peracctic acids were measured in the rock-salt region. The most significant features are theO-Hstretching frequency a t 3310-3350 cm.-1 and the O H bending frequency a t 1450 cm.-1 which, for both peracids, remain essentially the same in the vapor state a s in the liquid or in solution in nonpolar solvents. This is attributed to i~~tramolecular hydrogen bonds resulting in particularly stable five-membered rings, 0 .Steric conditions in the percarboxylic group are favorable to such ring formation or chelatiotz. From the observed freqi~ency shifts the energy of these hydrogen bonds is estimated to be about 7 kcal. per mole. No evidence for unchelated molecules was found even in very dilute solutions of peracetic acid in nonpolar solvent nor in the vapor a t low pressure and moderate temperature. Tentative assignments of the other freqiiencies i n the spectra of the peracids are made by comparison with those of formic and acetic acids.The danger involved in handling these peracids in concentrated form is emphasized.

show abstract

“…Titration by the methods of Swern (1953) showed the reagent to be 40 per cent. (w/v) peracetic acid with 6 per cent.…”

Section: Methodsmentioning

confidence: 99%

Studies on Oxidised Wool. I. A Comparison of the Completeness of Oxidation With Peracetic and Performic Acid

Thompson¹,

O'donnell²

1959

Aust. Jnl. Of Bio. Sci.

View full text Add to dashboard Cite

SummaryThe oxidation of wool with performic and peracetic acids has been compared by measuring the cysteic acid contents of hydrolysates of the oxidized wool. Whereas virtually complete oxidation takes place with performic acid, the oxidation with peracetic acid is incomplete. Performic acid can also further oxidize the partial oxidation products in wool treated with peracetic acid and it is concluded that performic acid is the more powerful oxidizing agent for wool. For the oxidation of the disulphide bonds prior to the extraction of proteins from wool, performic acid is therefore the better reagent.

show abstract

Organic Peracids.

Cited by 243 publications

References 118 publications

Fluorescent Assay for Directed Evolution of Perhydrolases

Fluorescent Assay for Directed Evolution of Perhydrolases

A Spectroscopic Study of Hydrogen Bonding in Performic and Peracetic Acids

Studies on Oxidised Wool. I. A Comparison of the Completeness of Oxidation With Peracetic and Performic Acid

Contact Info

Product

Resources

About