Organic Osmoregulatory Solutes in Blue-green Algae

Erdmann, Norbert

doi:10.1016/s0044-328x(83)80161-5

Cited by 35 publications

(22 citation statements)

References 17 publications

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“…), glucosylglycerol was accumulated by more salt-tolerant strains, typical of a marine environment, and quaternary nitrogen-containing compounds such as glycine-betaine or L-glutamate-betaine, either alone or in combination with sucrose and/or trehalose, were the major organic solutes accumulated by hypersaline strains. Glucosylglycerol accumulation by cyanobacteria under conditions of osmotic stress has also been noted by Erdmann (1983), Richardson et al (1983) and Reed et al (1984).…”

Section: Introductionmentioning

confidence: 82%

13C Nuclear Magnetic Resonance Study of Biosynthesis of Glucosylglycerol by a Cyanobacterium under Osmotic Stress

Mackay

Norton

1987

Microbiology

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3C nuclear magnetic resonance spectroscopy was used to study the biosynthesis and turnover of 0-a-D-glucopyranosyl-( 1+2)-glycerol (glucosylglycerol) in the marine cyanobacterium Synechococcus NlOO during osmotic shock. There was a rapid increase in the level of this heteroside following hyperosmotic shock, with most of the solute being synthesized from extracellular carbon via photosynthetic C 0 2 fixation. However, changes in the 3C labelling patterns during the time course of the response suggested that up to 10% of the newly synthesized material may be derived from intracellular carbon. No increase in glucosylglycerol was observed when cells were subjected to hyperosmotic shock in the dark. These experiments indicated that a good yield of highly 3C-enriched glucosylglycerol, which could serve as a source of labelled glucose and glycerol, could be obtained simply and quickly by subjecting this cyanobacterium to hyperosmotic stress in the presence of 90% 3C-enriched bicarbonate.

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Section: Introductionmentioning

confidence: 82%

13C Nuclear Magnetic Resonance Study of Biosynthesis of Glucosylglycerol by a Cyanobacterium under Osmotic Stress

Mackay

Norton

1987

Microbiology

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“…TLC was performed in acetonitrile/water (85 : 15, v/v) and repeated three times with careful drying after each run. For the determination of the whole spectrum of reaction products, aliquots of the enzyme assays were additionally separated by two-dimensional TLC (see Erdmann, 1983). Authentic "C-labelled G G was obtained from l4C-labe1led saltadapted cells of Microcystis firma after purification by ionexchange chromatography (Hagemann e t al., 1987).…”

Section: Methodsmentioning

confidence: 99%

“…One of about 30 such compounds known today is the heteroside O-GGDglucopyranosyl-( 1 + 2)-glycerol (glucosylglycerol ; GG) (Reed & Stewart, 1988;Truper & Galinski, 1989). G G accumulates mostly in cyanobacteria in response to external salt concentration (Borowitzka e t al., 1980 ;Erdmann, 1983) and enables the organisms to survive moderate salinities (Mackay e t al., 1983 ;Reed e t al., 1984). G G is absent in unstressed cells and is synthesized immediately after a salt shock (Erdmann et al, 1992) to an intracellular concentration which corresponds to the strength of the stress, and without marked turnover (Hagemann e t al., 1987).…”

Section: Introductionmentioning

confidence: 99%

Activation and pathway of glucosylglycerol synthesis in the cyanobacterium Synechocystis sp. PCC 6803

1994

Self Cite

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The biosynthetic pathway of glucosylglycerol (GG), the osmoprotective compound of Synechocystis sp. PCC 6803, was found to proceed from ADPglucose and glycerol 3-phosphate via glucosylglycerol phosphate in a two-step reaction. Using an in vitro assay it was shown that the GG-forming enzyme system required activation, which could be initiated in vivo by hypertonic salt concentrations and osmotic shock, or in vitro by NaCl addition at the stage of enzyme extraction or assay.

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“…Therefore, glucose was estimated ¢rst [27]. Then for the determination of GG and sucrose, respectively [7,24]. Aliquots of above ethanol extraction were acidi¢ed with HCl to a ¢nal concentration of 0.5 N and hydrolyzed at 100 ‡C for 120 and 30 min, respectively.…”

Section: Extraction and Determination Of Gg And Sucrosementioning

confidence: 99%

Sucrose accumulation in salt-stressed cells of agp gene deletion-mutant in cyanobacterium Synechocystis sp. PCC 6803

Miao

2003

FEMS Microbiology Letters

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The agp gene encoding the ADP-glucose pyrophosphorylase is involved in cyanobacterial glycogen synthesis and glucosylglycerol formation. By in vitro DNA recombination technology, a mutant with partial deletion of agp gene in the cyanobacterium Synechocystis sp. PCC 6803 was constructed. This mutant could not synthesize glycogen or the osmoprotective substance glucosylglycerol. In the mutant cells grown in the medium containing 0.9 M NaCl for 96 h, no glucosylglycerol was detected and the total amount of sucrose was 29 times of that of in wild-type cells. Furthermore, the agp deletion mutant could tolerate up to 0.9 M salt concentration. Our results suggest that sucrose might act as a similar potent osmoprotectant as glucosylglycerol in cyanobacterium Synechocystis sp. PCC 6803.

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Organic Osmoregulatory Solutes in Blue-green Algae

Cited by 35 publications

References 17 publications

13C Nuclear Magnetic Resonance Study of Biosynthesis of Glucosylglycerol by a Cyanobacterium under Osmotic Stress

13C Nuclear Magnetic Resonance Study of Biosynthesis of Glucosylglycerol by a Cyanobacterium under Osmotic Stress

Activation and pathway of glucosylglycerol synthesis in the cyanobacterium Synechocystis sp. PCC 6803

Sucrose accumulation in salt-stressed cells of agp gene deletion-mutant in cyanobacterium Synechocystis sp. PCC 6803

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