Organic loading rate and hydraulic retention time shape distinct ecological networks of anaerobic digestion related microbiome

Xu, Rui; Yang, Zhen; Zheng, Yue; Li, Jianbo; Xiong, Weiping; Zhang, Yanru; Lu, Yue; Xue, Wenjing; Fan, Changzheng

doi:10.1016/j.biortech.2018.04.083

Cited by 106 publications

(33 citation statements)

References 44 publications

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“…The methanogenic archaeal community mainly consisted of members of the genera Methanosarcina [33][34][35]. The Methanobacteria, Methanomicrobiales, and/or Methanomassiliicoccaceae members were also observed in certain AD-processes featuring a high organic load, depending on other prevailing conditions [36].…”

Section: Discussionmentioning

confidence: 99%

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Impact of process temperature and organic loading rate on cellulolytic / hydrolytic biofilm microbiomes during biomethanation of ryegrass silage revealed by genome-centered metagenomics and metatranscriptomics

Maus

Klocke

Derenkó

et al. 2020

Environmental Microbiome

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Background: Anaerobic digestion (AD) of protein-rich grass silage was performed in experimental two-stage twophase biogas reactor systems at low vs. increased organic loading rates (OLRs) under mesophilic (37°C) and thermophilic (55°C) temperatures. To follow the adaptive response of the biomass-attached cellulolytic/hydrolytic biofilms at increasing ammonium/ammonia contents, genome-centered metagenomics and transcriptional profiling based on metagenome assembled genomes (MAGs) were conducted.

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Section: Discussionmentioning

confidence: 99%

“…Based on this information, phylogenetic and functional molecular ecological networks (pMEN resp. fMEN) can be developed resulting in the definition of core microbiomes comprising key species for AD [36,[40][41][42]. Alternatively, a network of metabolic functionality independent from microbial community structure can be established [43].…”

Section: Discussionmentioning

confidence: 99%

Impact of process temperature and organic loading rate on cellulolytic / hydrolytic biofilm microbiomes during biomethanation of ryegrass silage revealed by genome-centered metagenomics and metatranscriptomics

Maus

Klocke

Derenkó

et al. 2020

Environmental Microbiome

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“…These can be the addition of certain beneficial microorganisms (bioaugmentation) [ 13 ] and/or the application of selection pressure, i.e., choosing specific process parameters, to favor their growth. Such environmental selection pressures can be, for example, temperature [ 14 , 15 ], ammonia concentration [ 16 ], feed composition [ 17 ], hydraulic retention time (HRT) [ 18 ], organic loading rate (OLR) [ 19 ], or the feeding regime in terms of temporal feeding schedule [ 1 , 20 – 24 ]. A temporal feeding schedule is particularly interesting because it could be implemented in industrial practice without additional investments [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Intermittent fasting for microbes: how discontinuous feeding increases functional stability in anaerobic digestion

Bonk

Popp

Weinrich

et al. 2018

Biotechnol Biofuels

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BackgroundDemand-driven biogas production could play an important role for future sustainable energy supply. However, feeding a biogas reactor according to energy demand may lead to organic overloading and, thus, to process failures. To minimize this risk, digesters need to be actively steered towards containing more robust microbial communities. This study focuses on acetogenesis and methanogenesis as crucial process steps for avoiding acidification. We fed lab-scale anaerobic digesters with volatile fatty acids under various feeding regimes and disturbances. The resulting microbial communities were analyzed on DNA and RNA level by terminal restriction fragment length polymorphism of the mcrA gene, 16S rRNA gene amplicon sequencing, and a [2-13C]-acetate assay. A modified Anaerobic Digestion Model 1 (ADM1) that distinguishes between the acetoclastic methanogens Methanosaeta and Methanosarcina was developed and fitted using experimental abiotic and biotic process parameters.ResultsDiscontinuous feeding led to more functional resilience than continuous feeding, without loss in process efficiency. This was attributed to a different microbial community composition. Methanosaeta dominated the continuously fed reactors, while its competitor Methanosarcina was washed out. With discontinuous feeding, however, the fluctuating acetic acid concentrations provided niches to grow and co-exist for both organisms as shown by transcription analysis of the mcrA gene. Our model confirmed the higher functional resilience due to the higher abundance of Methanosarcina based on its higher substrate uptake rate and higher resistance to low pH values. Finally, we applied our model to maize silage as a more complex and practically relevant substrate and showed that our model is likely transferable to the complete AD process.ConclusionsThe composition of the microbial community determined the AD functional resilience against organic overloading in our experiments. In particular, communities with higher share of Methanosarcina showed higher process stability. The share of these microorganisms can be purposefully increased by discontinuous feeding. A model was developed that enables derivation of the necessary feeding regime for a more robust community with higher share of Methanosarcina.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1279-5) contains supplementary material, which is available to authorized users.

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“…As the feeding-in of biomethane into the natural gas net allows for a conversion into electricity at larger scale, combined cycle gas turbines with conversion efficiencies of above 60%, electric efficiencies may increase compared to the local, small scale power generation units that are currently common [149]. • Variable feeding (feedstock amount and composition) to the digester or an adapted temperature regime can regulate the amount of methane produced within the biogas plant [49,51,[178][179][180][181]. This option reduces necessary investments for flexibilization [176], but requires a resilient microbiome (Section 3.2.2.)…”

Section: Biogas Supply On Demandmentioning

confidence: 99%

The Future Agricultural Biogas Plant in Germany: A Vision

et al. 2019

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After nearly two decades of subsidized and energy crop-oriented development, agricultural biogas production in Germany is standing at a crossroads. Fundamental challenges need to be met. In this article we sketch a vision of a future agricultural biogas plant that is an integral part of the circular bioeconomy and works mainly on the base of residues. It is flexible with regard to feedstocks, digester operation, microbial communities and biogas output. It is modular in design and its operation is knowledge-based, information-driven and largely automated. It will be competitive with fossil energies and other renewable energies, profitable for farmers and plant operators and favorable for the national economy. In this paper we discuss the required contribution of research to achieve these aims.

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Organic loading rate and hydraulic retention time shape distinct ecological networks of anaerobic digestion related microbiome

Cited by 106 publications

References 44 publications

Impact of process temperature and organic loading rate on cellulolytic / hydrolytic biofilm microbiomes during biomethanation of ryegrass silage revealed by genome-centered metagenomics and metatranscriptomics

Impact of process temperature and organic loading rate on cellulolytic / hydrolytic biofilm microbiomes during biomethanation of ryegrass silage revealed by genome-centered metagenomics and metatranscriptomics

Intermittent fasting for microbes: how discontinuous feeding increases functional stability in anaerobic digestion

The Future Agricultural Biogas Plant in Germany: A Vision

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