Organic loading rate and food-to-microorganism ratio shape prokaryotic diversity in a demo-scale up-flow anaerobic sludge blanket reactor treating domestic wastewater

Cardinali-Rezende, Juliana; Araújo, Juliana Calábria de; Almeida, Pedro Machado; Chernicharo, C. A. L.; Sanz, J. L.; Chartone-Souza, Edmar; Nascimento, Andréa M. A.

doi:10.1007/s10482-013-0018-y

Cited by 23 publications

(9 citation statements)

References 38 publications

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“…Some previous studies confirmed that the F/M ratio shaped the microbial abundance and composition in sludge bioreactors (38). In the present study, the F/M ratio was maintained at a low level of 0.05 to 0.13 kg BOD kg Ϫ1 mixed liquor suspended solids (MLSS) day Ϫ1 , which would hinder the growth of bacterial communities in high-elevation WWTPs.…”

Section: Resultssupporting

confidence: 81%

Understanding the Linkage between Elevation and the Activated-Sludge Bacterial Community along a 3,600-Meter Elevation Gradient in China

Niu

Wang

et al. 2015

Appl Environ Microbiol

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To understand the relationship between elevation and bacterial communities in wastewater treatment plants (WWTPs), bacterial communities in 21 municipal WWTPs across China, located 9 to 3,660 m above sea level (masl), were investigated by 454 pyrosequencing. A threshold for the association of elevation with bacterial community richness and evenness was observed at approximately 1,200 masl. At lower elevations, both richness and evenness were not significantly associated with elevation. At higher elevations, significant declines with increased elevations were observed for community richness and evenness. The declining evenness trend at the phylum level was reflected by distinct trends in relative abundance for individual bacterial phyla. Betaproteobacteria, Bacteroidetes, and Firmicutes displayed significant increases, while most other phyla showed declines. Spearman correlation analysis indicated that the community richness and evenness at high elevations were more correlated with elevation than with any other single environmental variable. Redundancy analysis indicated that the contribution of elevation to community composition variances increased from 3% at lower elevations to 11% at higher elevations whereas the community composition variance at higher elevations remained much more explained by operational variables (39.2%) than by elevation. The influent total phosphorus concentration, food/microorganism ratio, and treatment process were the three shared dominant contributors to the community composition variance across the whole elevation gradient, followed by effluent ammonia nitrogen and temperature at higher elevations. W astewater treatment plants (WWTPs) are broadly applied for the efficient removal of contaminants from wastewater before its discharge into the natural environment. A complex microbial community is the most dominant contributor to the removal of contaminants in WWTPs. A better understanding of the microbial community structure and its relationship to influential variables could provide important guidance for the improvement of wastewater treatment efficiency (1). Multiple studies have investigated the significant associations between WWTP microbial community structures and operational parameters or wastewater characteristics, such as dissolved oxygen concentration (2), temperature (3), treatment process (4), influent ammonia nitrogen concentration (5), biological oxygen demand (6), and total phosphorus (TP) concentration (7). However, regardless of the weakly neutral assembly of bacterial communities (8), there is still a large part of the bacterial community variance that cannot be explained by these well-known variables. Thus, it is presumed that there are still other environmental variables that potentially influence the variance of WWTP microbial communities.In recent years, a few studies were performed to explore the relationship between microbial communities and geographical locations of WWTPs. Zhang et al. (9) found significant geographical differences in bacterial communities amo...

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Section: Resultssupporting

confidence: 81%

Understanding the Linkage between Elevation and the Activated-Sludge Bacterial Community along a 3,600-Meter Elevation Gradient in China

Niu

Wang

et al. 2015

Appl Environ Microbiol

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“…1), which means that the relative abundances were more evenly distributed among taxa for such communities. On the contrary, prior studies reported a decrease in Shannon diversity [66,67] and Hill numbers [61] at higher organic loading. Since the low organic loading reactors displayed better COD removal and complete nitrification with almost no residual NH3-N or NO2 --N, it was expected that they would harbour more diverse communities, as more even communities were reported to have better functionality [68].…”

Section: Higher -Diversity Does Not Translate Into Better Functionmentioning

confidence: 66%

Press disturbance unveils different community structure, function and assembly of bacterial taxa and functional genes in mesocosm-scale bioreactors

Santillan

Constancias

Wuertz

2019

Preprint

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Sustained perturbations, or press disturbances, are of interest in microbial ecology as they can drive systems to alternative stable states. Here, we tested the effect of a sustained doubling of organic carbon loading on structure, assembly and function of bacterial communities. Two sets of replicate 5-liter sequencing batch reactors were operated at two different organic carbon loading levels (323 and 629 mg COD L -1 ) for a period of 74 days, following 53 days of acclimation after inoculation with sludge from a full-scale treatment plant. Temporal dynamics of community taxonomic and functional gene structure were derived from metagenomics and 16S rRNA gene metabarcoding data. Assembly mechanisms were assessed through a mathematical null model on the effective bacterial turnover expressed as a proportion of total bacterial diversity. Disturbed reactors exhibited different community function, structure and assembly compared to the undisturbed reactors. Bacterial taxa and functional genes showed dissimilar alphadiversity and community assembly patterns. Deterministic assembly mechanisms were generally stronger in disturbed reactors, associated with common taxa. Stochastic assembly was more important for functional genes and was driven by rare genes. We urge caution when assessing microbial community assembly mechanisms, as results can vary depending on the approach.

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“…The high abundance and strong shifts in the phyla Bacteroidetes, Firmicutes, and Proteobacteria dominated the composition of the common microbiome in the BW module [21]. This is because Bacteroidetes and Firmicutes are capable of degrade various types of cellulose, mainly from toilet paper [18].…”

Section: Discussionmentioning

confidence: 99%

On-Site Blackwater Treatment Fosters Microbial Groups and Functions to Efficiently and Robustly Recover Carbon and Nutrients

Kuramae

Dimitrov

Silva

et al. 2020

Preprint

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Background: Wastewater is considered as a renewable resource water and energy. An advantage of decentralized sanitation systems is the separation of the blackwater (BW) stream, which is highly contaminated with human pathogens, from the remaining household water. However, the composition and functions of the microbial community in BW are not known. In this study, we used shotgun metagenomics to assess the dynamics of microbial community structure and function throughout a new BW anaerobic digestion system installed at The Netherlands Institute of Ecology. Samples from the influent (BW), primary effluent (anaerobic digested BW), sludge and final effluent of the pilot upflow anaerobic sludge blanket (UASB) reactor and microalgae pilot tubular photobioreactor (PBR) were analyzed.Results: Our results showed a decrease in microbial richness and diversity followed by a decrease in functional complexity and co-occurrence along the different modules of the bioreactor. The microbial diversity and function decrease were reflected both changes in substrate composition and wash conditions. The most prevalent core functions in influent (BW) were related to metabolism of carbohydrates, response to chemicals and drugs, and nitrogen. The core functions in anaerobic digested BW and upflow anaerobic sludge blanket reactor were related to response to stress, viral processes and iron-sulfur metabolism. Methanogenesis-related functions were most abundant in upflow anaerobic sludge blanket reactor. Effluent from tubular photobioreactor presented high abundances of functions related to nitrogen utilization, metal ion binding and antibiotic biosynthetic processes. Interestingly, the abundance of sequences related to ‘pathogenesis’ decreased from influent BW to SP1 to effluent from tubular photobioreactor. Our wastewater treatment system also decreased potential microbial functions related to pathogenesis. Conclusions: The new sanitation system studied here fosters microbial groups and functions that allow the system to efficiently and robustly recover carbon and nutrients while reducing pathogenic groups, ultimately generating a final effluent safe for discharge and reuse.

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Organic loading rate and food-to-microorganism ratio shape prokaryotic diversity in a demo-scale up-flow anaerobic sludge blanket reactor treating domestic wastewater

Cited by 23 publications

References 38 publications

Understanding the Linkage between Elevation and the Activated-Sludge Bacterial Community along a 3,600-Meter Elevation Gradient in China

Understanding the Linkage between Elevation and the Activated-Sludge Bacterial Community along a 3,600-Meter Elevation Gradient in China

Press disturbance unveils different community structure, function and assembly of bacterial taxa and functional genes in mesocosm-scale bioreactors

On-Site Blackwater Treatment Fosters Microbial Groups and Functions to Efficiently and Robustly Recover Carbon and Nutrients

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