Organic light emitting diode improves diabetic cutaneous wound healing in rats

Abstract

A major complication for diabetic patients is chronic wounds due to impaired wound healing. It is well documented that visible red wavelengths can accelerate wound healing in diabetic animal models and patients. In vitro and in vivo diabetic models were used to investigate the effects of organic light emitting diode (OLED) irradiation on cellular function and cutaneous wound healing. Human dermal fibroblasts were cultured in hyperglycemic medium (glucose concentration 180 mM) and irradiated with an OLED (623 nm wavelength peak, range from 560 to 770 nm, power density 7 or 10 mW/cm2 at 0.2, 1, or 5 J/cm2). The OLED significantly increased total adenosine triphosphate concentration, metabolic activity, and cell proliferation compared with untreated controls in most parameters tested. For the in vivo experiment, OLED and laser (635 ± 5 nm wavelength) treatments (10 mW/cm2 , 5 J/cm2 daily for a total of seven consecutive days) for cutaneous wound healing were compared using a genetic, diabetic rat model. Both treatments had significantly higher percentage of wound closure on day 6 postinjury and higher total histological scores on day 13 postinjury compared with control. No statistical difference was found between the two treatments. OLED irradiation significantly increased fibroblast growth factor-2 expression at 36-hour postinjury and enhanced macrophage activation during initial stages of wound healing. In conclusion, the OLED and laser had comparative effects on enhancing diabetic wound healing.

Heparin‐poloxamer hydrogel‐encapsulated rhFGF21 enhances wound healing in diabetic mice

“…Under severe conditions, chronic diabetic ulcers of the feet and lower extremities will develop, and many patients with diabetes eventually require amputation. Traditional treatments for diabetic wounds are timeconsuming and laborious, and the results are often unsatisfactory (3,6). In addition, several previous studies have reported new nonpharmacological therapies, such as hyperbaric oxygen therapy, photobiomodulation and low-level laser therapy (34).…”

“…Wounds in patients with diabetes heal more slowly or are nonhealing. Moreover, persistent chronic skin ulcers are present in diabetic skin wounds (3). Impaired wound healing has complex underlying pathogeneses, such as a prolonged inflammatory response, inadequate angiogenesis, and decreased fibroblast mitogenesis and growth factor production (4).…”

Improved Wound Remodeling Correlates with Modulated TGF‐beta Expression in Skin Diabetic Wounds Following Combined Red and Infrared Photobiomodulation Treatments

“…These studies have implicated decreased pro‐inflammatory cytokines (TNF α , IL1 β , IL6), mast cell degranulation, altered MMPs and redox status, decreased cell adhesion complexes as well as modulated bFGF signaling as key players in PBM modulated DM wound healing . There have been specific studies designed to assess the effects of wavelengths, dose, light sources (including OLEDs), pulsing as well as combinations with nutritional supplements such as CoQ10 that have all shown positive correlations .…”

“…These studies have implicated decreased pro-inflammatory cytokines (TNFa, IL1b, IL6), mast cell degranulation, altered MMPs and redox status, decreased cell adhesion complexes as well as modulated bFGF signaling as key players in PBM modulated DM wound healing (22)(23)(24)(25)(26)(27). There have been specific studies designed to assess the effects of wavelengths, dose, light sources (including OLEDs), pulsing as well as combinations with nutritional supplements such as CoQ10 that have all shown positive correlations (12,(28)(29)(30)(31)(32)(33)(34)(35)(36). Histological assessment (criteria outlined in Table S2) for re-epithelization and inflammation was performed in each treatment group at day 4 and 10 PBM post-treatments.…”

22‐3: Distinquished Student Paper: Flexible Quantum Dot Light Emitting Devices for Photomedicine

“…PBM with red light has been demonstrated to improve diabetic wound healing by accelerating collagen production, enhancing angiogenesis, increasing wound closure rate, and increasing growth factor expression. While OLEDs have been applied to improve diabetic cutaneous wound healing in rats, their broad emission peaks and relatively low power density remains a major factor limiting their treatment effects [12,13].…”