Organic and inorganic content of fluorotic rat incisors measured by FTIR spectroscopy

Porto, Isabel Maria; Saiani, Regina Aparecida Segatto; Chan, K. L. Andrew; Kazarian, Sergei G.; Gerlach, Raquel Fernanda; Bachmann, Luciano

doi:10.1016/j.saa.2010.04.024

Cited by 12 publications

(11 citation statements)

References 17 publications

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“…On the other hand, fluorotic enamel samples do present more intense C-H signal in the late stage of maturation, especially on surface enamel. This observation is compatible with the increased protein content found in rat incisors, originated in the presence of high fluoride concentrations as compared with rat incisors formed under control conditions (12). Nonetheless, protein (or peptides) retention in later stages of enamel maturation does not necessarily imply a causal relationship with enamel defects.…”

Section: Introductionsupporting

confidence: 81%

Peptide Characterization of Mature Fluorotic and Control Human Enamel

Lelis

Molina

Souza³

et al. 2016

Braz. Dent. J.

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Exposure to high fluoride levels during amelogenesis causes enamel fluorosis. This study aimed to determine and compare the amino acid sequences in the enamel of fluorotic and control teeth. This investigation included enamel samples obtained from erupted and non-erupted third molars with either TF grade 4-6 (n=7) fluorosis or no sign of fluorosis (controls, n=7). The samples were kept frozen at -20 °C until protein extraction. Samples were etched and processed with a cocktail of proteinase inhibitors and immediately analyzed. Matrix Assisted Laser Desorption/Ionization-Time-Of-Flight/Time-of-Flight Mass Spectrometry (MALDI-TOF/TOF) followed by MASCOT search aided the peptides analysis. The more abundant peptides bore the N-terminal amelogenin sequences WYQSIRPPYP (which is specific for the X-encoded amelogenin) and MPLPPHPGHPGYINF (which does not show sexual dimorphism) were not different in control or fluorotic enamel. There was no missing proteolytic cleavage in the fluorotic samples, which suggested that the increased amount of protein described in fluorotic enamel did not stem from the decreased ability of proteinases to cleave the proteins in humans. This study showed how to successfully obtain peptide from superficial enamel. A relatively low number of teeth was sufficient to provide good data on the actual peptides found in mature enamel.

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Section: Introductionsupporting

confidence: 81%

Peptide Characterization of Mature Fluorotic and Control Human Enamel

Lelis

Molina

Souza³

et al. 2016

Braz. Dent. J.

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“…www.nature.com/scientificreports/ It should be noted that the intensity of the mode 1401.1 cm −1 and the shift of its position relative to the literature value of 1420 cm −1 (Figs. 5a and 6a) is due to the presence of a small amount of organic component in the enamel associated with CH group, the most intensive lines of which (symmetric CH 3 bending, stretching of COO) arise in the region 1412-1396 cm −1 , and overlap with the band of CO 3 2− ν 3 34,37,40 . However, the analysis of the low-intensity vibrational bands of CO 3 ν 1 in IR reflection spectra in the region of 890-860 cm −1 , where (in contrast to the Raman spectra) there is no overlap with other vibrations (Table 1), showed a similar trend in the transformation of intensities for A and B-type carbonate substitutions in fluorotic enamel (Figs.…”

Section: Discussionmentioning

confidence: 99%

“…At the same time using the IR-microspectroscopy with synchrotron radiation as a source allows one to analyze biological specimens (human tooth tissues) and pathology processes in them with a greater lateral resolution during data acquisition and high signal-to-noise ratio without the long-term accumulation of the desired signal that is important for the study of biological specimens [36][37][38] . The use of infrared microspectroscopy with synchrotron radiation makes it possible to clarify the molecular composition and study local changes in the mineral-organic matrix of dental enamel from the areas less than 10 µm 239 .…”

Section: This Study Is Aimed At Investigating the Features Of Mineralmentioning

confidence: 99%

“…The use of infrared microspectroscopy with synchrotron radiation makes it possible to clarify the molecular composition and study local changes in the mineral-organic matrix of dental enamel from the areas less than 10 µm 2 39 . Moreover, in any case the applicability of synchrotron IR-microspectroscopy to the dental tissue analysis is more convenient than Raman microspectroscopy, even though the Raman microscopy is also an excellent approach to vibrational spectroscopy, with an equivalent, or often a better, spatial resolution than synchrotron infrared microscopy 31 , 33 , 37 , 40 .…”

Section: Introductionmentioning

confidence: 99%

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Development of a new approach to diagnosis of the early fluorosis forms by means of FTIR and Raman microspectroscopy

Seredin

Goloshchapov

Ippolitov

et al. 2020

Sci Rep

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This study is aimed at investigating the features of mineralization of the enamel apatite at initial stages of fluorosis development. Samples of teeth with intact and fluorotic enamel in an early stage of the disease development (Thylstrup–Fejerskov Index = 1–3) were studied by Raman scattering and FTIR using Infrared Microspectroscopy beamline at Australian Synchrotron equipment. Based on the data obtained by optical microspectroscopy and calculation of the coefficient R [A-type/B-type], which represents the ratio of carbonation fraction of CO32−, replacing phosphate or hydroxyl radicals in the enamel apatite lattice, the features of mineralization of enamel apatite in the initial stages of development of the pathology caused by an increased content of fluorine in the oral cavity were established. Statistical analysis of the data showed significant differences in the mean values of R [A-type/B-type] ratio between the control and experimental groups for surface layers (p < 0.01). The data obtained are potentially significant as benchmarks in the development of a new approach to preventive diagnostics of the development of initial and clinically unregistered stages of human teeth fluorosis, as well as personalized control of the use of fluoride-containing caries-preventive agents.

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“…ATR-FTIR spectroscopic imaging has been developed in the last decade and applied to investigate biomedical samples and art materials (Spring et al, 2008;Porto et al, 2010;Kazarian and Chan, 2013). This mode of measurement requires minimal preparation as analyses are realized at the surface of the samples.…”

Section: Introductionmentioning

confidence: 99%