A 3-year field tillage and residue management experiment established in North China was used to analyse topsoil (0-15 cm) aggregation, and microbial functional diversity, enzyme activity and glomalin-related soil protein (GRSP) content within aggregates. Compared with conventional tillage (CT), no-tillage (NT) alone significantly (P < 0.05) increased organic C contents in 50-250 and <2 lm aggregates and decreased the proportion of C accumulated by 2-50 lm aggregates and microbial functional diversity indices in <2 lm aggregates. Regardless of tillage practice, both half-amount (C50) and full (C100) residue retention tended to increase organic C and GRSP contents, or dehydrogenase and invertase activities, in certain aggregates. Under CT, a poorer performance of C50 than C100 was observed in maintaining Shannon index (H 0 ) and Simpson index (D) in >250 and <2 lm aggregates, and also McIntosh index (U) in <2 lm aggregates, owing to insufficient residue and possible decreases in the distribution of decomposer micro-organisms. Under NT, however, C50 was more effective than C100 in maintaining/elevating H 0 , D and U in all soil aggregates except for 50-250 lm, suggesting that surplus residue may induce worse soil conditions, decreasing heterotrophic microbial activities. Thus, NT with half-amount residue retention improved soil physical-chemicalbiological properties and could be a useful management practice in North China.