Organic additives stabilize RNA aptamer binding of malachite green

Aptamer adaptive binding assessed by stilbene photoisomerization towards regenerating aptasensors

“…Photochrome aptamer switch assay (PHASA) concept, design and preparation of the elements, and the long-term stability of the RNA aptamer have previously been reported [26,36,37]. Our previous investigation focused on the synthesis of stilbene maleimide derivatives and prepared an aptamer-stilbene based on maleimide-thiol conjugation.…”

“…The combination of MGA with its triphenylmethane ligands, as seen in Fig. 2, was chosen as a model study for a number of reasons: 1) the combination allows rapid screening of binding affinities in high throughput microplate assays [36], 2) MGA has a host of known diaryl-and triarylmethane ligands (see Fig. 2) that bind at varying affinities [38][39][40], 3) MGA's 3D crystal structure and NMR aqueous adaptive binding conformation have already been established [39,41] and 4) the triarylmethane, an MG ligand, has commercial significance as a pigment, illegal fungicide, and blood detection compound [42][43][44].…”

On-Demand Bioadhesive Dendrimers with Reduced Cytotoxicity

“…However, the degree of infiltration of giant cells was reduced to none for PAMAM-g-diazirine possibly due to the germicidal ability of –NH 2 groups [ 35 , 39 ]. These results indicate that the tissue adhesive warrants further investigation towards vascular patches, drug depots, and hydrogel biosensor implants [ 17 , 18 , 40 , 41 , 42 , 43 , 44 , 45 , 46 ].…”

Current state of in vivo panning technologies: Designing specificity and affinity into the future of drug targeting

“…Additionally, their small size contributes to their rapid renal filtration. To prevent degradation, aptamer libraries have been modified chemically to replace the 2’ position with a fluoro (F), amino (NH 2 ), or O-methyl (OCH 3 ) group, and by capping the 3’ end with inverted thymidine[99, 112]. While chemical modifications can impart improved stability and circulation, chemical modification to the aptamer backbone or side-chains can affect aptamer folding and binding.…”