Organic Acids Production and Phosphate Solubilization by Phosphate Solubilizing
Microorganisms (PSM) Under in vitro Conditions

Rashid, Maliha; Khalil, Samina; Ayub, Najma; Alam, Sadia; Latif, Farooq

doi:10.3923/pjbs.2004.187.196

Cited by 278 publications

(63 citation statements)

References 33 publications

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“…In the present study, a significant negative relationship was observed between the amount of soluble P and pH in the culture medium (R 2 =0.953). This result reaffirms that phosphate solubilization by PSB is involved in the production of organic acids (Halder et al, 1990;Goldstein, 1995;Kim et al, 1998;Rashid et al, 2004). The recent research demonstrated that citric acid secreted by PSB strains can improve their P-solubilizing activity.…”

Section: Discussionsupporting

confidence: 58%

“…P-solubilizing activity is related to the microbial production of organic acids, which chelate the cation bound to phosphate, thereby converting it to a soluble form (Sagoe et al, 1998;Rashid et al, 2004;Lugtenberg & Kamilova, 2009). In the present study, a significant negative relationship was observed between the amount of soluble P and pH in the culture medium (R 2 =0.953).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers

Ling

Liu

Cheng

et al. 2017

Span. j. agric. res.

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Phosphate-solubilizing bacteria (PSB) have the ability to solubilize insoluble phosphorus (P) and release soluble P. Extensive research has been performed with respect to PSB isolation from the rhizospheres of various plants, but little is known about the prevalence of PSB in the grapevine rhizosphere. In this study, we aimed to isolate and identify PSB from the grapevine rhizosphere in five vineyards of Northwest China, to characterize their plant-growth-promoting (PGP) traits, evaluate the effect of stress on their phosphate-solubilizing activity (PSA), and test their ability to stimulate the growth of Vitis vinifera L. cv. Cabernet Sauvignon. From the vineyard soils, 66 PSB isolates were screened, and 10 strains with high PSA were identified by 16S rRNA sequencing. Sequence analysis revealed that these 10 strains belonged to 4 genera and 5 species: Bacillus aryabhattai, B. megaterium, Klebsiella variicola, Stenotrophomonas rhizophila, and Enterobacter aerogenes. The selected PSB strains JY17 (B. aryabhattai) and JY22 (B. aryabhattai) were positive for multiple PGP traits, including nitrogen fixation and production of indole acetic acid (IAA), siderophores, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, chitinase, and protease. JY17 and JY22 showed strong PSA under stress conditions of high pH, high salt, and high temperature. Therefore, these two isolates can be used as biofertilizers in saline-alkaline soils. The inoculation with PSB significantly facilitated the growth of V. vinifera cv. Cabernet Sauvignon under greenhouse conditions. Use of these PSB as biofertilizers will increase the available P content in soils, minimize P-fertilizer application, reduce environmental pollution, and promote sustainable agriculture.Additional key words: promote plant growth; inorganic phosphate; stress conditions; saline-alkaline soil; Vitis vinifera L. cv. Cabernet SauvignonAbbreviations used: ACC (1-aminocyclopropane-1-carboxylate); CAS (chrome azurol S); DF (Dworkin-Foster); GADH (gluconate dehydrogenase); IAA (indole acetic acid); NBRIP (National Botanical Research Institute's phosphate); PGP (plant-growthpromoting); PQQ-GDH (pyrroloquinoline quinone-dependent glucose dehydrogenase); PSA (phosphate-solubilizing activity); PSB (phosphate-solubilizing bacteria); TCP (tricalcium phosphate).Authors' contributions: ML drafted the manuscript; XL made critical revision of the manuscript for important intellectual content; BSC and XLM acquired, analyzed and interpreted data; XTL coordinated the research project; XFZ performed statistical analysis; YLJ and ZM proposed revision suggestions; YLF supervised the work.Citation: Liu, M.; Liu, X.; Cheng, B. S.; Ma, X. L.; Lyu, X. T.; Zhao, X. F.; Ju, Y. L.; Min, Z.; Fang, Y. L. (2016). Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers.

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers

Ling

Liu

Cheng

et al. 2017

Span. j. agric. res.

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“…Actually, P-solubilizer microorganisms have been described at this niche (Whitelaw, 1999; Linu et al, 2009; Silva et al, 2014; Matthews and Adzahar, 2016). Organic acid production has been pointed as the main factor involved in RP microbial solubilization (Rashid et al, 2004; Mendes et al, 2014). Nevertheless, other mechanisms, such as the release of H + ions during NH 4 + assimilation or metabolic reactions that trigger proton excretion such as cellular respiration, can also contribute to P solubilization (Illmer and Schinner, 1995).…”

Section: Introductionmentioning

confidence: 99%

Long-Term Rock Phosphate Fertilization Impacts the Microbial Communities of Maize Rhizosphere

Silva

Medeiros²,

Leite³

et al. 2017

Front. Microbiol.

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Phosphate fertilization is a common practice in agriculture worldwide, and several commercial products are widely used. Triple superphosphate (TSP) is an excellent soluble phosphorus (P) source. However, its high cost of production makes the long-term use of crude rock phosphate (RP) a more attractive alternative in developing countries, albeit its influence on plant-associated microbiota remains unclear. Here, we compared long-term effects of TSP and RP fertilization on the structure of maize rhizosphere microbial community using next generation sequencing. Proteobacteria were dominant in all conditions, whereas Oxalobacteraceae (mainly Massilia and Herbaspirillum) was enriched in the RP-amended soil. Klebsiella was the second most abundant taxon in the RP-treated soil. Burkholderia sp. and Bacillus sp. were enriched in the RP-amended soil when compared to the TSP-treated soil. Regarding fungi, Glomeromycota showed highest abundance in RP-amended soils, and the main genera were Scutellospora and Racocetra. These taxa are already described as important for P solubilization/acquisition in RP-fertilized soil. Maize grown on TSP and RP-treated soil presented similar productivity, and a positive correlation was detected for P content and the microbial community of the soils. The results suggest changes of the microbial community composition associated to the type of phosphate fertilization. Whilst it is not possible to establish causality relations, our data highlights a few candidate taxa that could be involved in RP solubilization and plant growth promotion. Moreover, this can represent a shorter path for further studies aiming the isolation and validation of the taxa described here concerning P release on the soil plant system and their use as bioinoculants.

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“…amoriaceae (Giorgio 2014;Giorgio et al 2013). The six antagonistic rhizobacteria showed also desirable plant growthpromoting ability since they can supply inorganic phosphate to plants (Schachtman et al 1998) via phosphatase activity which leads to the production of organic acids that decrease soil pH (Rashid et al 2004), so that this may limit some soilborne phytopathogenic organisms, as demonstrated in the case of P. ultimum and F. oxysporum (Alhussaen 2012). However, a direct effect of rhizobacteria on the pathogen at the infection site cannot be excluded since it has been reported that bacteria applied to soil or on seeds colonise rhizosphere and then may move up to the phylloplane (Nautiyal et al 2002) or to behave as either facultative or opportunistic endophytes (Hardoim et al 2008).…”

Section: Fagioli DI Sarconi Seed Disinfectionmentioning

confidence: 99%

The Sustainability of Agro-Food and Natural Resource Systems in the Mediterranean Basin

Vastola¹

2015

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Organic Acids Production and Phosphate Solubilization by Phosphate Solubilizing Microorganisms (PSM) Under in vitro Conditions

Cited by 278 publications

References 33 publications

Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers

Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers

Long-Term Rock Phosphate Fertilization Impacts the Microbial Communities of Maize Rhizosphere

The Sustainability of Agro-Food and Natural Resource Systems in the Mediterranean Basin

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