Organic acids as a substitute for monensin in diets for beef cattle

Castillo, Cristina; Benedito, José Luís; Méndez, J.; Pereira, Víctor; López‐Alonso, Marta; Miranda, Marta; Hernández, Joaquín

doi:10.1016/j.anifeedsci.2004.02.001

Cited by 105 publications

(61 citation statements)

References 36 publications

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“…(Morgavi et al, 2010). Among them fumarate is non-toxic and an intermediate of the pathways of propionate formation (Russell and Wallace, 1997), and has been extensively studied as an alternative electron sink (Castillo et al, 2004). Fumarate has been associated with favorable changes in ruminal fermentation in vitro as well as in vivo (Asanuma et al, 1999a;Ungerfeld et al, 2007;Wood et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Effects of disodium fumarate on ruminal fermentation and microbial communities in sheep fed on high-forage diets

Zhou

McSweeney

Wang

et al. 2012

Animal

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This study was conducted to investigate effects of disodium fumarate (DF) on fermentation characteristics and microbial populations in the rumen of Hu sheep fed on high-forage diets. Two complementary feeding trials were conducted. In Trial 1, six Hu sheep fitted with ruminal cannulae were randomly allocated to a 2 3 2 cross-over design involving dietary treatments of either 0 or 20 g DF daily. Total DNA was extracted from the fluid-and solid-associated rumen microbes, respectively. Numbers of 16S rDNA gene copies associated with rumen methanogens and bacteria, and 18S rDNA gene copies associated with rumen protozoa and fungi were measured using real-time PCR, and expressed as proportion of total rumen bacteria 16S rDNA. Ruminal pH decreased in the DF group compared with the control (P , 0.05). Total volatile fatty acids increased (P , 0.001), but butyrate decreased (P , 0.01). Addition of DF inhibited the growth of methanogens, protozoa, fungi and Ruminococcus flavefaciens in fluid samples. Both Ruminococcus albus and Butyrivibrio fibrisolvens populations increased (P , 0.001) in particle-associated samples. Trial 2 was conducted to investigate the adaptive response of rumen microbes to DF. Three cannulated sheep were fed on basal diet for 2 weeks and continuously for 4 weeks with supplementation of DF at a level of 20 g/day. Ruminal samples were collected every week to analyze fermentation parameters and microbial populations. No effects of DF were observed on pH, acetate and butyrate (P . 0.05). Populations of methanogens and R. flavefaciens decreased in the fluid samples (P , 0.001), whereas addition of DF stimulated the population of solid-associated Fibrobacter succinogenes. Population of R. albus increased in the 2nd to 4th week in fluid-associated samples and was threefold higher in the 4th week than control week in solid samples. Analysis of denaturing gradient gel electrophoresis fingerprints revealed that there were significant changes in rumen microbiota after adding DF. Ten of 15 clone sequences from cut-out bands appearing in both the 2nd and the 4th week were 94% to 100% similar to Prevotella-like bacteria, and four sequences showed 95% to 98% similarity to Selenomonas dianae. Another 15 sequences were obtained from bands, which appeared in the 4th week only. Thirteen of these 15 sequences showed 95% to 99% similarity to Clostridium sp., and the other two showed 95% and 100% similarity to Ruminococcus sp. In summary, the microorganisms positively responding to DF addition were the cellulolytic bacteria, R. albus, F. succinogenes and B. fibrisolvens as well as proteolytic bacteria, B. fibrisolvens, P. ruminicola and Clostridium sp.

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Section: Introductionmentioning

confidence: 99%

Effects of disodium fumarate on ruminal fermentation and microbial communities in sheep fed on high-forage diets

Zhou

McSweeney

Wang

et al. 2012

Animal

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“…Bannink et al (2005) included the effect of ruminal pH on VFA stoichiometric parameters related to type of substrate fermented, which indicated the profound effect of type of substrate fermented as well as of rumen pH on predicted methane yield (Table 2). Some mitigation strategies to reduce methane emissions, including the addition of fats (in particular unsaturated fatty acids and lauric and myristic acids; Giger-Reverdin et al (2003)) and of organic acids (including fumarate and malate; Castillo et al (2004)), are not yet covered by mechanistic models, although they affect the profile of nutrients available for absorption. Similarly, reduction in methane emissions from the rumen may be Predicting the profile of absorbed nutrients partly offset by increased emissions from hindgut fermentation or subsequently in slurry methanogenesis (Hindrichsen et al, 2006), which for predictions on the animal sublevel require models of hindgut fermentation.…”

Section: Environmental Pollutionmentioning

confidence: 99%

Predicting the profile of nutrients available for absorption: from nutrient requirement to animal response and environmental impact

Dijkstra

Kebreab

Mills

et al. 2007

Animal

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Current feed evaluation systems for dairy cattle aim to match nutrient requirements with nutrient intake at pre-defined production levels. These systems were not developed to address, and are not suitable to predict, the responses to dietary changes in terms of production level and product composition, excretion of nutrients to the environment, and nutrition related disorders. The change from a requirement to a response system to meet the needs of various stakeholders requires prediction of the profile of absorbed nutrients and its subsequent utilisation for various purposes. This contribution examines the challenges to predicting the profile of nutrients available for absorption in dairy cattle and provides guidelines for further improved prediction with regard to animal production responses and environmental pollution.The profile of nutrients available for absorption comprises volatile fatty acids, long-chain fatty acids, amino acids and glucose. Thus the importance of processes in the reticulo-rumen is obvious. Much research into rumen fermentation is aimed at determination of substrate degradation rates. Quantitative knowledge on rates of passage of nutrients out of the rumen is rather limited compared with that on degradation rates, and thus should be an important theme in future research. Current systems largely ignore microbial metabolic variation, and extant mechanistic models of rumen fermentation give only limited attention to explicit representation of microbial metabolic activity. Recent molecular techniques indicate that knowledge on the presence and activity of various microbial species is far from complete. Such techniques may give a wealth of information, but to include such findings in systems predicting the nutrient profile requires close collaboration between molecular scientists and mathematical modellers on interpreting and evaluating quantitative data. Protozoal metabolism is of particular interest here given the paucity of quantitative data.Empirical models lack the biological basis necessary to evaluate mitigation strategies to reduce excretion of waste, including nitrogen, phosphorus and methane. Such models may have little predictive value when comparing various feeding strategies. Examples include the Intergovernmental Panel on Climate Change (IPCC) Tier II models to quantify methane emissions and current protein evaluation systems to evaluate low protein diets to reduce nitrogen losses to the environment. Nutrient based mechanistic models can address such issues. Since environmental issues generally attract more funding from governmental offices, further development of nutrient based models may well take place within an environmental framework.

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“…An in vitro study performed by Martin and Streeter (1995) showed that the effect of free malic acid in the rumen was similar to that of disodium malate, although differences in chemical structure might imply different effects on ruminal fermentation. Free acids provide protons to the medium, which could decrease ruminal pH, whereas the contribution of the sodium cation in the salts can help to increase the pH (Castillo et al, 2004). Nisbet and Martin (1994) reported a synergistic action of both forms of malate, and observed that sodium concentrations between 25 and 100 mM stimulated consumption of L-lactate in the presence of S. ruminantium and 10 mM of malate ion.…”

Section: Organic Acids and Their Saltsmentioning

confidence: 99%

“…Malate has been reported to stimulate in vitro lactate uptake by S. ruminantium (Martin and Park, 1996;Martin, 1998), and to increase ruminal pH and propionate production (reviewed by Carro and Ungerfeld, 2015). In contrast, information on the effects of malate on in vivo ruminal fermentation and performance of beef cattle is more limited and conflicting (Castillo et al, 2004). Yeast cultures of Saccharomyces cerevisiae have also been reported to stimulate lactate utilization by S. ruminantium and M. elsdenii , and could contribute to increase rumen pH (Chaucheyras-Durand et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Malic acid is an organic acid naturally found in many fruits and plants, including forages used in ruminant feeding , which has been proposed as a substitute for monensin in beef cattle diets because its effects on ruminal fermentation are similar to those of ionophores (Castillo et al, 2004). Although malic acid and malate salts have been repeatedly shown to have positive effects on in vitro ruminal fermentation by increasing ruminal pH and propionate production (Carro and Ranilla, 2003;Gómez et al, 2005;Newbold et al, 2005;Tejido et al, 2006), available information on effects of malate on in vivo ruminal fermentation and ruminant performance is limited and conflicting.…”

Section: Introductionmentioning

confidence: 99%

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Nutritional strategies to control ruminal acidosis in feedlot cattle

Barco¹

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2017 I II IV AgradecimientosLlegar hasta este momento de finalización de la Tesis Doctoral ha sido una carrera de fondo que me ha acompañado a lo largo de mi maduración tanto a nivel profesional como personal, acompañándome en la mochila en numerosos momentos clave de mi vida.Todo empezó nada más presentar mi proyecto fin de carrera, cuando Carlos de Blas, (que de manera sutil, o quizá involuntaria, ha tenido la capacidad de llevarme a lo que soy ahora) comentó "dos artículos más y ya tienes la Tesis". Si bien en aquel momento me pareció una idea imposible, en el fondo se quedó plantada una semilla que comenzó a germinar el día en el que Pedro Medel me encargó realizar el estado del arte del proyecto de investigación que, con el soporte de COPESE, ha dado lugar a este trabajo.Recuerdo perfectamente aquel primer momento en el que visitamos la granja de terneras por primera vez y Javier Peinado dejó en mis manos el control de los ensayos.Llegar hasta el final de este proyecto hubiera sido imposible sin el apoyo tanto de mis compañeros de Imasde en aquellos momentos (Álvaro, Silvia, Jaime, Nacho y Fran), como de David del Pico, Marcos Nieto y Alfonso Fuentetaja de COPESE y, por supuesto, los profesores, investigadores y doctorandas del Departamento de Producción Animal I de la Universidad de León (Loli, Mariajo, Marisa, Eugenia, Silvia, Alfonso..) por su apoyo tanto en granja como en matadero, y especialmente en laboratorio.Quedan atrás muchos días de frío, me atrevería a decir casi siberiano, donde antes de empezar los ensayos algún día nos encontramos a Javier Rincón descongelando tuberías a soplete; aquellos momentos en los que una ternera despistada nos hacía correr los San Fermines por los pasillos, o los madrugones para poder realizar los controles en matadero. Son muchas las anécdotas que quedan atrás, y guardo con buen sabor de boca, un poco de nostalgia, pero especialmente satisfacción por todo lo vivido y aprendido, junto con el sincero agradecimiento a todos los que me han acompañado en este camino. En este proceso de aprendizaje, cabe sin duda la mención especial a mis directores, Pedro y Loli que, sin prisa pero sin pausa, me han impulsado a finalizar este trabajo.Los trabajos realizados en el marco de esta Tesis Doctoral han sido promovidos por Comercial Pecuaria Segoviana, SL (Coca, Segovia, Spain), y llevados a cabo en su granja experimental de terneros de cebo. Este proyecto ha sido financiado por el Ministerio de Industria Turismo y Comercio mediante el programa PROFIT (FIT060000200511) y el Centro para el Desarrollo Tecnológico Industrial (CDTI; IDI-20050617 RESUMENEl objetivo de esta Tesis ha sido estudiar la influencia de diferentes estrategias nutricionales sobre el control de la acidosis en terneros de cebo en un contexto experimental diseñado para representar el manejo nutricional predominante en España. Para ello, se llevaron a cabo tres ensayos experimentales que se describen a continuación.El primer experimento se diseñó para evaluar el efecto de la inclusión de malato, administra...

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Organic acids as a substitute for monensin in diets for beef cattle

Cited by 105 publications

References 36 publications

Effects of disodium fumarate on ruminal fermentation and microbial communities in sheep fed on high-forage diets

Effects of disodium fumarate on ruminal fermentation and microbial communities in sheep fed on high-forage diets

Predicting the profile of nutrients available for absorption: from nutrient requirement to animal response and environmental impact

Nutritional strategies to control ruminal acidosis in feedlot cattle

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