Organelle imaging with carbon dots: strategies, challenges, and perspectives

Abstract

Organelle imaging is an efficient approach to gain information about intracellular events and dynamics of subcellular structures.

Comprehensive review on fluorescent carbon dots and their applications in nucleic acid detection, nucleolus targeted imaging and gene delivery

“…110 It is pertinent to note that understanding the cellular uptake pathways of CDs is crucial for assessing their biological properties and optimizing their biomedical applications. 25…”

“…However, it has been reported that CDs synthesized from identical precursors and uniform conditions were shown to exhibit diverse organelle-targeting properties. 25 As a result, further comprehensive studies focusing on preparation methodologies are vital to enhance the organelle selectivity of intrinsic nucleolus-targeting CDs.…”

“…CDs with intrinsic organelle-targeting capabilities, not requiring post-modification steps, are getting significant attention in organelle-specific imaging studies owing to their unique surface functional groups, zeta potential, amphiphilic nature, and good photophysical properties. 25 It has been reported that CDs synthesized from one-pot methods typically retain specific properties from the starting precursor molecules. Therefore, a careful selection of precursors that possess inherent organelle-targeting features is crucial for achieving intrinsic organelle-targeting capabilities in CDs.…”

“…While previous reviews have covered different aspects of CDs, such as synthesis, surface functionalization, photoluminescence properties, and biological applications, ongoing research continues to explore and expand the potential of CDs in various biomedical fields. 23–27 This review offers an overview of the latest advancements in CD-based gene detection, gene delivery and nucleolus imaging applications. The discussion encompasses the use of CDs in the detection of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) for disease diagnosis.…”

Effects of multifunctional cerium-doped carbon dots on photosynthetic capacity and nutritional quality of lettuce

“…As a new type of nanomaterial, carbon dots (CDs) are usually spherical particles with a particle size of less than 10 nm, and have good water solubility, 13 photoluminescence, 14 easy regulation, 15 low toxicity, 16 and biocompatibility. 17,18 CDs have been widely used in cosmetics, 19 cell imaging, 20 sensing, 21 information encryption, 22 and other fields. Researchers have shown great interest in the multi-functionalization of CDs and have begun to explore and apply it in the fields of agriculture.…”