Organ-specific transcriptome response of the small brown planthopper toward rice stripe virus

Zhao, Wan; Lu, Lixia; Yang, Pengcheng; Cui, Na; Kang, Le; Cui, Feng

doi:10.1016/j.ibmb.2015.11.009

Cited by 43 publications

(44 citation statements)

References 68 publications

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“…After a BLAST search of the small brown planthopper transcriptome (Zhao et al, 2016a) with the partial GPS2 sequence, the full-length open reading frame (ORF) of the planthopper GPS2 was identified and cloned. Its 1299-bp nucleotides encode a 432 amino acid residue protein (GenBank accession KY435901).…”

Section: Resultsmentioning

confidence: 99%

“…Planthopper GPS2 was most similar to that of the termite Zootermopsis nevadensis (Figure 1—figure supplement 1). A paralogous gene, GPS1 (GenBank accession KY435902), which clustered with mammalian GPS1 gene (Figure 1—figure supplement 1), was also identified in the small brown planthopper transcriptome (Zhao et al, 2016a). Planthopper GPS1 and GPS2 had 17% amino acid sequence identity and GPS1 had no coiled-coil domain.…”

Section: Resultsmentioning

confidence: 99%

“…Two JNK genes were identified in the small brown planthopper transcriptome (Zhao et al, 2016a) using three human JNKs (GenBank accessions P45983, P45984, and P53779) as queries in BLASTp searches. The ORFs of the two putative JNKs were 1287 bp and 1125 bp, encoding a 49 kD protein (JNK1, GenBank accession KY435903) and a 43 kD protein (JNK2, GenBank accession KY435904), respectively.…”

Section: Resultsmentioning

confidence: 99%

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The c-Jun N-terminal kinase pathway of a vector insect is activated by virus capsid protein and promotes viral replication

Wang

Zhao

et al. 2017

eLife

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No evidence has shown whether insect-borne viruses manipulate the c-Jun N-terminal kinase (JNK) signaling pathway of vector insects. Using a system comprising the plant virus Rice stripe virus (RSV) and its vector insect, the small brown planthopper, we have studied the response of the vector insect’s JNK pathway to plant virus infection. We found that RSV increased the level of Tumor Necrosis Factor-α and decreased the level of G protein Pathway Suppressor 2 (GPS2) in the insect vector. The virus capsid protein competitively bound GPS2 to release it from inhibiting the JNK activation machinery. We confirmed that JNK activation promoted RSV replication in the vector, whereas JNK inhibition caused a significant reduction in virus production and thus delayed the disease incidence of plants. These findings suggest that inhibition of insect vector JNK may be a useful strategy for controling the transmission of plant viruses.DOI: http://dx.doi.org/10.7554/eLife.26591.001

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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The c-Jun N-terminal kinase pathway of a vector insect is activated by virus capsid protein and promotes viral replication

Wang

Zhao

et al. 2017

eLife

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“…Non‐viruliferous fourth‐instar nymphs were fed on an artificial diet containing the JSHA isolate of RSV crude preparations from the infected rice leaves, as described previously (Zhao et al ., 2016a). After feeding on RSV for 8 h, the nymphs were transferred to healthy rice seedlings and then collected after 2, 5, 8, 10, 12, 14 and 22 d. Four replicates and five insects per replicate at each time point were prepared.…”

Section: Methodsmentioning

confidence: 99%

“…Although RSV can replicate in both vector insects and host plants, different symptoms are induced in the two types of host. Instead of producing severe diseases as it does in host plants, RSV seems to live in harmony with planthoppers without causing intense immune responses or inducing obvious damage (Zhang et al ., 2010; Zhao et al ., 2016a). Moreover, the RSVs from planthoppers and from rice plants exhibit different pathogenicities.…”

Section: Introductionmentioning

confidence: 99%

Genomic variations in the 3′‐termini of Rice stripe virus in the rotation between vector insect and host plant

Zhao

Zhang

et al. 2018

New Phytologist

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Summary A large number of plant RNA viruses circulate between plants and insects. For RNA viruses, host alternations may impose a differential selective pressure on viral populations and induce variations in viral genomes. Here, we report the variations in the 3′‐terminal regions of the multiple‐segment RNA virus Rice stripe virus (RSV) that were discovered through de novo assembly of the genome using RNA sequencing data from infected host plants and vector insects.The newly assembled RSV genome contained 16‐ and 15‐nt extensions at the 3′‐termini of two genome segments compared with the published reference RSV genome. Our study demonstrated that these extensional sequences were consistently observed in two RSV isolates belonging to distinct genetic subtypes in RSV‐infected rice, wheat and tobacco. Moreover, the de novo assembled genome of Southern rice black‐streaked dwarf virus also contained 3′‐terminal extensions in five RNA segments compared with the reference genome.Time course experiments confirmed that the 3′‐terminal extensions of RSV were enriched in the vector insects, were gradually eliminated in the host plant and potentially affected viral replication.These findings indicate that variations in the 3′‐termini of viral genomes may be different adaptive strategies for plant RNA viruses in insects and plants.

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Plant–Virus–Vector Interactions

et al. 2021

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Organ-specific transcriptome response of the small brown planthopper toward rice stripe virus

Cited by 43 publications

References 68 publications

The c-Jun N-terminal kinase pathway of a vector insect is activated by virus capsid protein and promotes viral replication

The c-Jun N-terminal kinase pathway of a vector insect is activated by virus capsid protein and promotes viral replication

Genomic variations in the 3′‐termini of Rice stripe virus in the rotation between vector insect and host plant

Plant–Virus–Vector Interactions

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