Organ-Specific Splice Variants of Aquaporin Water Channel AgAQP1 in the Malaria Vector Anopheles gambiae

Tsujimoto, Hitoshi; Liu, Kun; Linser, Paul J.; Agre, Peter; Rasgon, Jason L.

doi:10.1371/journal.pone.0075888

Cited by 36 publications

(40 citation statements)

References 32 publications

(41 reference statements)

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“…Similarly, splice variants have been reported for human kidney AQP2 (Ma et al, 1996), human brain mitochondria AQP9 (Amiry-Moghaddam et al, 2005), rat AQP4 (Moe et al, 2008), Anopheles gambiae aquaporin 1 (Tsujimoto et al, 2013), Lygus hesperus…”

Section: Discussionmentioning

confidence: 56%

“…For example, in A. gambiae, the aquaporin 1 variant A is specifically expressed in female ovaries, while variant B is expressed in the midgut, hindgut, Malpighian tubules and the head (Tsujimoto et al, 2013). Rat AQP4 has 6 isoforms (a-f) and some are localized intracellularly and do not transport water when expressed in X. laevis oocytes (Moe et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

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Molecular and functional characterization of Bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects

Ekert

Chauvigné

Finn

et al. 2016

Insect Biochemistry and Molecular Biology

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The Middle East-Asia Minor 1 (MEAM1) whitefly, Bemisia tabaci (Gennadius) is an economically important pest of food, fiber, and ornamental crops. This pest has evolved a number of adaptations to overcome physiological challenges, including 1) the ability to regulate osmotic stress between gut lumen and hemolymph after imbibing large quantities of a low nitrogen, sugar-rich liquid diet; 2) the ability to avoid or prevent dehydration and desiccation, particularly during egg hatching and molting; and 3) to be adapted for survival at elevated temperatures. One superfamily of proteins involved in the maintenance of fluid homeostasis in many organisms includes the aquaporins, which are integral membrane channel proteins that aid in the rapid flux of water and other small solutes across biological membranes. Here, we show that B. tabaci has eight aquaporins (BtAqps), of which seven belong to the classical aquaporin 4-related grade of channels, including Bib, Drip, Prip, and Eglps and one that belongs to the unorthodox grade of aquaporin 12-like channels. B. tabaci has further expanded its repertoire of water channels through the expression of three BtDrip2 amino-terminal splice variants, while other hemipteran species express amino- or carboxyl-terminal isoforms of Drip, Prip, and Eglps. Each BtAqp has unique transcript expression profiles, cellular localization, and/or substrate preference. Our phylogenetic and functional data reveal that hemipteran insects lost the classical glp genes, but have compensated for this by duplicating the eglp genes early in their evolution to comprise at least three separate clades of glycerol transporters.

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Molecular and functional characterization of Bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects

Ekert

Chauvigné

Finn

et al. 2016

Insect Biochemistry and Molecular Biology

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“…Conversely, knockdown of Drip increased survival of both Ae. aegypti (Drake et al, 2015) and Anopheles gambiae (Liu et al, 2011;Tsujimoto et al, 2013) under dry conditions.…”

Section: Several Putative T Castaneum Aquaporins Appear To Be Functimentioning

confidence: 99%

RNA interference‐mediated functional characterization of aquaporin genes in Tribolium castaneum

Yao

Meng

2017

Insect Molecular Biology

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An obvious challenge faced by most terrestrial insects is maintaining water homeostasis in an arid environment. Current research suggests aquaporins may be evolved to meet the challenge. However, up to now, this suggestion has not been verified in any insect that feeds upon solid food with mandibulate mouthparts. In the present paper, nine putative aquaporin genes [Tribolium castaneum big brain, T. castaneum Drosophila integral protein (TcDrip), T. castaneum Pyrocoelia rufa integral protein (TcPrip), T. castaneum aquaporin 12-like, T. castaneum entomoglyceroporin 1 (TcEglp1), TcEglp2, TcEglp3, TcEglp4 and TcEglp5] were identified in T. castaneum. The transcripts of the nine genes were easily detectable in the foregut, midgut, hindgut-Malpighian tubule complex, fat body and carcass (except gut and fat body). Amongst them TcDrip, TcPrip, TcEglp1, TcEglp3 and TcEglp5 were highly transcribed in the hindgut-Malpighian tubule complex; TcEglp4 was abundantly expressed in both the fat body and hindgut-Malpighian tubule complex. RNA interference (RNAi)-mediated knockdown of TcEglp3 caused a grey larval cuticle, in contrast to a smooth and bright cuticle in control larvae. Approximately 40% of the TcEglp3 RNAi larvae had their hindguts protruding from the anus; their fresh wet faeces were attached to the hindgut. Another 20% of these treated larvae did not defaecate normally; wet brown faeces were adhered to the anal area. As a result, the larval growth was inhibited and about 60% larval lethality occurred. Silencing of TcEglp4 or TcDrip exhibited similar but weaker defective phenotypes as those of the TcEglp3-silenced larvae. Therefore, Eglp3, Eglp4 and Drip may contribute to the conductance of water in the alimentary canal and Malpighian tubules in T. castaneum.

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“…In Drosophila, this transport is also dependent on basolateral Na + /K + ATPases for moving Na + , K + and Cl − into the cell (see Ianowski and O'Donnell, 2004, O'Donnell et al, 2003and Ramsay, 1954. When combined, this active ion transport establishes an osmotic gradient that allows water to enter the tubule lumen from the hemolymph, aided by aquaporins expressed by principle and stellate cells (Kaufmann et al, 2005;Misyura et al, 2017;Spring et al, 2009;Tsujimoto et al, 2013). Some K + and water is reabsorbed in the lower segment of the Malpighian tubules, before passing into the hindgut through the ureter (O'Donnell and Maddrell, 1995;Rheault and O'Donnell, 2001).…”

Section: Introductionmentioning

confidence: 99%

Cold tolerance ofDrosophilaspecies is tightly linked to epithelial K+ transport capacity of the Malpighian tubules and rectal pads

Andersen

MacMillan

Donini

et al. 2017

Journal of Experimental Biology

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Insect chill tolerance is strongly associated with the ability to maintain ion and water homeostasis during cold exposure. Maintenance of K balance is particularly important due to its role in setting the cell membrane potential that is involved in many aspects of cellular function and viability. In most insects, K balance is maintained through secretion at the Malpighian tubules, which balances reabsorption from the hindgut and passive leak arising from the gut lumen. Here, we used the scanning ion-selective electrode technique (SIET) at benign (23°C) and low (6°C) temperatures to examine K flux across the Malpighian tubules and the rectal pads in the hindgut in five species that differ in cold tolerance. We found that chill-tolerant species were better at maintaining K secretion and suppressing reabsorption during cold exposure. In contrast, chill-susceptible species exhibited large reductions in secretion with no change, or a paradoxical increase, in K reabsorption. Using an assay to measure paracellular leak, we found that chill-susceptible species experience a large increase in leak during cold exposure, which could explain the apparent increase in K reabsorption found in these species. Our data therefore strongly support the hypothesis that cold-tolerant species are better at maintaining K homeostasis through an increased ability to maintain K secretion rates and through reduced movement of K towards the hemolymph. These adaptations are manifested both at the Malpighian tubule and at the rectal pads in the hindgut, and ensure that cold-tolerant species experience less perturbation of K homeostasis during cold stress.

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Organ-Specific Splice Variants of Aquaporin Water Channel AgAQP1 in the Malaria Vector Anopheles gambiae

Cited by 36 publications

References 32 publications

Molecular and functional characterization of Bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects

Molecular and functional characterization of Bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects

RNA interference‐mediated functional characterization of aquaporin genes in Tribolium castaneum

Cold tolerance ofDrosophilaspecies is tightly linked to epithelial K+ transport capacity of the Malpighian tubules and rectal pads

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