Organ-specific metabolic responses to drought in Pinus pinaster Ait.

Miguel, Marina de; Guevara, M. Ángeles; Sánchez‐Gómez, David; María, Nuria de; Díaz, Luis Manuel; Mancha, José Antonio; Simón, Brígida Fernández de; Cadahía, Estrella; Desai, Nalini; Aranda, Ismael; Cervera, Marı́a Teresa

doi:10.1016/j.plaphy.2016.02.013

Cited by 54 publications

(45 citation statements)

References 42 publications

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“…In trees, the level of Asc also depends on the needle age. Adult needles of Pinus pinaster seedlings contained several times more Asc than juvenile needles [55]. The level of the AsA-based antioxidant response is also linked to the age of trees.…”

Section: Development Of Woody Plantsmentioning

confidence: 99%

Ascorbic Acid—The Little-Known Antioxidant in Woody Plants

et al. 2019

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Reactive oxygen species (ROS) are constantly produced by metabolically active plant cells. The concentration of ROS may determine their role, e.g., they may participate in signal transduction or cause oxidative damage to various cellular components. To ensure cellular homeostasis and minimize the negative effects of excess ROS, plant cells have evolved a complex antioxidant system, which includes ascorbic acid (AsA). AsA is a multifunctional metabolite with strong reducing properties that allows the neutralization of ROS and the reduction of molecules oxidized by ROS in cooperation with glutathione in the Foyer-Halliwell-Asada cycle. Antioxidant enzymes involved in AsA oxidation and reduction switches evolved uniquely in plants. Most experiments concerning the role of AsA have been performed on herbaceous plants. In addition to extending our understanding of this role in additional taxa, fundamental knowledge of the complex life cycle stages of woody plants, including their development and response to environmental factors, will enhance their breeding and amend their protection. Thus, the role of AsA in woody plants compared to that in nonwoody plants is the focus of this paper. The role of AsA in woody plants has been studied for nearly 20 years. Studies have demonstrated that AsA is important for the growth and development of woody plants. Substantial changes in AsA levels, as well as reduction and oxidation switches, have been reported in various physiological processes and transitions described mainly in leaves, fruits, buds, and seeds. Evidently, AsA exhibits a dual role in the photoprotection of the photosynthetic apparatus in woody plants, which are the most important scavengers of ozone. AsA is associated with proper seed production and, thus, woody plant reproduction. Similarly, an important function of AsA is described under drought, salinity, temperature, light stress, and biotic stress. This report emphasizes the involvement of AsA in the ecological advantages, such as nutrition recycling due to leaf senescence, of trees and shrubs compared to nonwoody plants.

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Section: Development Of Woody Plantsmentioning

confidence: 99%

Ascorbic Acid—The Little-Known Antioxidant in Woody Plants

et al. 2019

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“…In addition to the direct economic benefits provided by tree species, i.e., timber and non-timber products, gaming and tourism, forests have an immensurable ecological value, being the major determinants for water, oxygen, carbon, and energy balance and can be seen as a major opportunity to mitigate climate change effects [12], i.e., continued drought, increased soil and water salinization and acidification, and intensification of extreme temperatures [13]. In forest tree metabolomics research, most biological questions are indeed related to the responses towards the acclimation and adaptation to a permanently changing environment [14][15][16][17][18][19][20][21][22][23][24][25][26] as well as to the identification of potentially active components in tree species of pharmacological, agricultural, environmental, or industrial importance [27][28][29][30][31][32][33].…”

Section: Biological Question Formulationmentioning

confidence: 99%

“…In forest tree research, LC-MS instruments have also been used for untargeted secondary metabolite profiling and phytohormone quantification studies. The focus of these studies was related with abiotic stress responses [19][20][21][22][23][24]67,88,89]; and to a smaller extent to biotic stress responses [90,91] and plant growth and developmental processes [77,92,93].…”

Section: Lc-ms Metabolite Profilingmentioning

confidence: 99%

“…Metabolite extraction for LC-MS untargeted analysis are usually performed using a simple protocol based on methanol [19] or methanol:water 80:20, v/v [20,91] or 50:50, v/v [90] as extraction solvents. However, most untargeted secondary metabolite profiling studies in forest tree metabolomics research are performed in combination with GC-qMS or GC-TOF-MS primary metabolite profiling, ultimately allowing for more comprehensive coverage of the tree metabolome.…”

Section: Lc-ms Metabolite Profilingmentioning

confidence: 99%

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Experimental Design and Sample Preparation in Forest Tree Metabolomics

2019

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Appropriate experimental design and sample preparation are key steps in metabolomics experiments, highly influencing the biological interpretation of the results. The sample preparation workflow for plant metabolomics studies includes several steps before metabolite extraction and analysis. These include the optimization of laboratory procedures, which should be optimized for different plants and tissues. This is particularly the case for trees, whose tissues are complex matrices to work with due to the presence of several interferents, such as oleoresins, cellulose. A good experimental design, tree tissue harvest conditions, and sample preparation are crucial to ensure consistency and reproducibility of the metadata among datasets. In this review, we discuss the main challenges when setting up a forest tree metabolomics experiment for mass spectrometry (MS)-based analysis covering all technical aspects from the biological question formulation and experimental design to sample processing and metabolite extraction and data acquisition. We also highlight the importance of forest tree metadata standardization in metabolomics studies.

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“…Also, Meijón et al 394(2016) showed that the metabolomes in needles of maritime pine trees from populations with distinct 395 geographic origin (notably Atlantic versus Mediterranean provenances) were quite differentiated and 396 flavonoids showed a significant correlation with the water regime of the population of origin. 397However, the expression of metabolites is organ specific(de Miguel et al, 2016) and knowledge about 398 secondary metabolites involved in resistance to D. sapinea is still lacking. 399A study on the invasive pathogen Fusarium circinatum, which did certainly not co-evolve with 400 maritime pine, also revealed a geographic cline in susceptibility, with Atlantic maritime pine 401populations showing less susceptibility than Moroccan populations (Elvira-Recuenco et al, 2014).…”

mentioning

confidence: 99%

Genetic basis of growth, phenology and susceptibility to biotic stressors in maritime pine

Hurel

Miguel

Dutech

et al. 2019

Preprint

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SummaryForest ecosystems are increasingly challenged by extreme events, e.g. pest and pathogen outbreaks, causing severe ecological and economical losses. Understanding the genetic basis of adaptive traits in tree species is of key importance to preserve forest ecosystemsAdaptive phenotypes, including susceptibility to two fungal pathogens (Diplodia sapinea and Armillaria ostoyae) and an insect pest (Thaumetopoea pityocampa), height and needle phenology were assessed in a range-wide common garden of maritime pine (Pinus pinaster Aiton), a widespread conifer in the western Mediterranean Basin and parts of the Atlantic coast.Broad-sense heritability was significant for height (0.497), needle phenology (0.231-0.468) and pathogen symptoms (0.413 for D. sapinea and 0.066 for A. ostoyae) measured after inoculation under controlled conditions, but not for pine processionary moth incidence assessed in the common garden. Genetic correlations between traits revealed contrasting trends for pathogen susceptibility to D. sapinea and A. ostoyae. Maritime pine populations from areas with high summer temperatures and frequent droughts were less susceptible to D. sapinea but more susceptible to A. ostoyae. An association study using 4,227 genome-wide SNPs revealed several loci significantly associated to each trait.This study provides important insights to develop genetic conservation and breeding strategies integrating tree responses to pathogens.

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Organ-specific metabolic responses to drought in Pinus pinaster Ait.

Cited by 54 publications

References 42 publications

Ascorbic Acid—The Little-Known Antioxidant in Woody Plants

Ascorbic Acid—The Little-Known Antioxidant in Woody Plants

Experimental Design and Sample Preparation in Forest Tree Metabolomics

Genetic basis of growth, phenology and susceptibility to biotic stressors in maritime pine

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