Organ‐specific, developmental, hormonal and stress regulation of expression of putative pectate lyase genes in Arabidopsis

Palusa, Saiprasad G.; Golovkin, Maxim; Shin, Sungbong; Richardson, Dale N.; Reddy, Anireddy S. N.

doi:10.1111/j.1469-8137.2007.02033.x

Cited by 65 publications

(83 citation statements)

References 77 publications

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“…PEL is an endogenous pectin-degrading enzyme that is capable of cleaving a-1,4-glycosidic linkages in demethylated pectin by b-elimination (Palusa et al, 2007). It is a ubiquitous enzyme in higher plants and is encoded by at least 26, 22, and 14 genes in Arabidopsis, poplar (Populus trichocarpa), and rice, respectively (Palusa et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…PELs, a family of endo-acting depolymerizing enzymes, are responsible for the a-1,4-glycosidic linkages in demethylesterified HG by b-elimination and produces 4,5-unsaturated oligogalacturonides (OGs) at their nonreducing ends (Palusa et al, 2007). PELs have been extensively studied in plant pathogenic bacteria such as Erwinia chrysanthemi, which causes soft-rot diseases (Barras et al, 1994).…”

mentioning

confidence: 99%

“…In plants, multiple functions have been identified for PEL, and there is some suggestion that it is involved in pollen, anthers, pistils, and developing tracheary elements (Wing et al, 1990;Rogers et al, 1992;Wu et al, 1996;Kulikauskas and McCormick, 1997;Domingo et al, 1998;Milioni et al, 2001), fruit softening and ripening (Dominguez-Puigjaner et al, 1997;Medina-Escobar et al, 1997;Nunan et al, 2001;Pua et al, 2001), lateral root emergence (Laskowski et al, 2006), cotton fiber elongation (Wang et al, 2010), leaf senescence (Wu et al, 2013), and susceptibility to plant pathogens (Vogel et al, 2002). It has also been shown to be expressed in a wide range of tissues (Palusa et al, 2007;Sun and van Nocker, 2010). In addition, recent research has revealed that the overexpression of aspen PtxtPL1-27 can increase the solubility of wood matrix polysaccharides (Biswal et al, 2014).…”

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confidence: 99%

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A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence

et al. 2017

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To better understand the molecular mechanisms behind plant growth and leaf senescence in monocot plants, we identified a mutant exhibiting dwarfism and an early-senescence leaf phenotype, termed dwarf and early-senescence leaf1 (del1). Histological analysis showed that the abnormal growth was caused by a reduction in cell number. Further investigation revealed that the decline in cell number in del1 was affected by the cell cycle. Physiological analysis, transmission electron microscopy, and TUNEL assays showed that leaf senescence was triggered by the accumulation of reactive oxygen species. The DEL1 gene was cloned using a map-based approach. It was shown to encode a pectate lyase (PEL) precursor that contains a PelC domain. DEL1 contains all the conserved residues of PEL and has strong similarity with plant PelC. DEL1 is expressed in all tissues but predominantly in elongating tissues. Functional analysis revealed that mutation of DEL1 decreased the total PEL enzymatic activity, increased the degree of methylesterified homogalacturonan, and altered the cell wall composition and structure. In addition, transcriptome assay revealed that a set of cell wall function-and senescence-related gene expression was altered in del1 plants. Our research indicates that DEL1 is involved in both the maintenance of normal cell division and the induction of leaf senescence. These findings reveal a new molecular mechanism for plant growth and leaf senescence mediated by PECTATE LYASE-LIKE genes.

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Section: Discussionmentioning

confidence: 99%

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A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence

et al. 2017

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“…It is well established that BRs promote cell elongation by inducing the expression of the genes involved in cell wall-loosening enzymes, such as xyloglucan endotransglycosylase/hydrolase (XTH) and Pectin Lyase-like (PLL) as well as expansins (EXP) (28)(29)(30). Consistent with its major function in promoting cell elongation, BRs induce the expression of 7 XTHs, 2 PLLs and 7 EXPs in adult plants.…”

Section: Most Of the Herk1/the1/fer Affected Genes Are Not Regulated Bymentioning

confidence: 98%

Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

Guo

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

331

357

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Cell elongation in plants is controlled by environmental cues such as light and internal growth regulators including plant steroid hormones, brassinosteroids (BRs). In this study, we found that 3 related receptor-like kinases (RLKs), HERCULES1, THESEUS1, and FERONIA, are transcriptionally induced by BRs and are downregulated in the loss-of-function BR mutant bri1 and up-regulated in the constitutive BR-response mutant bes1-D. These RLKs belong to the CrRLK family that has 17 members in Arabidopsis. We hypothesize that these RLKs are involved in BR-regulated processes. Although 2 of the RLKs were recently found to mediate male-female interaction during pollen tube reception (FERONIA) and to sense cell wall integrity (THESEUS1), our genetic studies demonstrated that they are required for cell elongation during vegetative growth as herk1 the1 double and fer RNAi mutants displayed striking dwarf phenotypes. The herk1 the1 double mutant enhances the dwarf phenotype of bri1 and partially suppresses bes1-D phenotype, supporting a role of HERK1/THE1 in BR-mediated cell elongation. Microarray experiments demonstrated that these RLKs control the expression of a unique set of genes including those implicated in cell elongation and 16% of the genes affected in herk1 the1 are regulated by BRs. Our results, therefore, identify a previously unknown pathway that functions cooperatively with, but largely independent of the BR pathway to regulate cell elongation. The work establishes a platform to identify other signaling components in this important pathway for plant growth and provides a paradigm to study the coordination of independent pathways in the regulation of a common biological process.brassinosteroid ͉ FERONIA ͉ HERCULES1 ͉ THESEUS1

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“…Supplemental Table S2 reveals transcript fold changes between floral stages for a subset of AZ 551 genes. Annotation conventions within Supplemental Table S2 include those of Kende et al (2004), Nakano et al (2006), Palusa et al (2007), and Rose et al (2002). Cluster 1 transcripts increase in abundance between stages 12 and 15a and remain at high levels as abscission commences near stages 15b and 15c (Fig.…”

Section: Lcm Facilitates Az Transcriptome Profilingmentioning

confidence: 99%

Stamen Abscission Zone Transcriptome Profiling Reveals New Candidates for Abscission Control: Enhanced Retention of Floral Organs in Transgenic Plants Overexpressing ArabidopsisZINC FINGER PROTEIN2

2008

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Organ detachment requires cell separation within abscission zones (AZs). Physiological studies have established that ethylene and auxin contribute to cell separation control. Genetic analyses of abscission mutants have defined ethylene-independent detachment regulators. Functional genomic strategies leading to global understandings of abscission have awaited methods for isolating AZ cells of low abundance and very small size. Here, we couple laser capture microdissection of Arabidopsis thaliana stamen AZs and GeneChip profiling to reveal the AZ transcriptome responding to a developmental shedding cue. Analyses focus on 551 AZ genes (AZ 551 ) regulated at the highest statistical significance (P # 0.0001) over five floral stages linking prepollination to stamen shed. AZ 551 includes mediators of ethylene and auxin signaling as well as receptor-like kinases and extracellular ligands thought to act independent of ethylene. We hypothesized that novel abscission regulators might reside in disproportionately represented Gene Ontology Consortium functional categories for cell wall modifying proteins, extracellular regulators, and nuclear-residing transcription factors. Promoter-b-glucuronidase expression of one transcription factor candidate, ZINC FINGER PROTEIN2 (AtZFP2), was elevated in stamen, petal, and sepal AZs. Flower parts of transgenic lines overexpressing AtZFP2 exhibited asynchronous and delayed abscission. Abscission defects were accompanied by altered floral morphology limiting pollination and fertility. Hand-pollination restored transgenic fruit development but not the rapid abscission seen in wild-type plants, demonstrating that pollination does not assure normal rates of detachment. In wild-type stamen AZs, AtZFP2 is significantly up-regulated postanthesis. Phenotype data from transgene overexpression studies suggest that AtZFP2 participates in processes that directly or indirectly influence organ shed.

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Organ‐specific, developmental, hormonal and stress regulation of expression of putative pectate lyase genes in Arabidopsis

Cited by 65 publications

References 77 publications

A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence

A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence

Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

Stamen Abscission Zone Transcriptome Profiling Reveals New Candidates for Abscission Control: Enhanced Retention of Floral Organs in Transgenic Plants Overexpressing ArabidopsisZINC FINGER PROTEIN2

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