Organ-on-a-chip with integrated semitransparent organic electrodes for barrier function monitoring

Marrero, Denise; Guimerà‐Brunet, Anton; Maes, Laure; Villa, Rosa; Álvarez, Mar; Illa, Xavi

doi:10.1039/d2lc01097f

Cited by 15 publications

(7 citation statements)

References 46 publications

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“…An EGTA‐dependent increase in cell layer capacitance has been reported previously in Caco‐2 monolayers and is hypothesized to take place due to transient rearrangements in actin microfilaments and cell morphology. [ 62 ] The large standard deviation in EGTA‐treated membrane capacitance values, but not resistance values (Figure 4E,F), have also been reported. [ 62 ] This may be due to the reduced ability to reliably detect changes in membrane capacitance, at low membrane resistances.…”

Section: Resultssupporting

confidence: 54%

“…[ 62 ] The large standard deviation in EGTA‐treated membrane capacitance values, but not resistance values (Figure 4E,F), have also been reported. [ 62 ] This may be due to the reduced ability to reliably detect changes in membrane capacitance, at low membrane resistances. [ 63 ] Additionally, since EGTA predominantly disrupts paracellular pathways, that is, resistance across the cell layer, and does not change cell number or thickness, capacitance may be a less reliable measurement of this specific chemically induced barrier disruption.…”

Section: Resultsmentioning

confidence: 55%

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A Conformable Organic Electronic Device for Monitoring Epithelial Integrity at the Air Liquid Interface

Barron,

Oldroyd,

Saez

et al. 2023

Advanced Materials

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Air liquid interfaced (ALI) epithelial barriers are essential for homeostatic functions such as nutrient transport and immunological protection. Dysfunction of such barriers are implicated in a variety of autoimmune and inflammatory disorders and, as such, sensors capable of monitoring barrier health are integral for disease modelling, diagnostics and drug screening applications. To date, gold‐standard electrical methods for detecting barrier resistance require rigid electrodes bathed in an electrolyte, which limits compatibility with biological architectures and is non‐physiological for ALI. This work presents a flexible all‐planar electronic device capable of monitoring barrier formation and perturbations in human respiratory and intestinal cells at ALI. By interrogating patient samples with electrochemical impedance spectroscopy and simple equivalent circuit models, disease‐specific and patient‐specific signatures are uncovered. Device readouts are validated against commercially available chopstick electrodes and show greater conformability, sensitivity and biocompatibility. The effect of electrode size on sensing efficiency is investigated and a cut‐off sensing area is established, which is one order of magnitude smaller than previously reported. This work provides the first steps in creating a physiologically relevant sensor capable of mapping local and real‐time changes of epithelial barrier function at ALI, which will have broad applications in toxicology and drug screening applications.

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Section: Resultssupporting

confidence: 54%

Section: Resultsmentioning

confidence: 55%

A Conformable Organic Electronic Device for Monitoring Epithelial Integrity at the Air Liquid Interface

Barron,

Oldroyd,

Saez

et al. 2023

Advanced Materials

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show abstract

“… Organ types Intestinal cells Co-cultured cells Microbes Materials Preparation technology Peristalsis Bioflow/μL min Electrodes TEER/Ω cm2 Findings Gut [17–19 [ [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] ], Caco-2 cells [ [17] , [18] , [19] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] ] N.A. LGG [ 33 ]; B. bifidum [ 17 ]; B.scentis , E.hallii [ 36 ]; Shigella flexneri [ 37 ]; Coxsackievirus B1 [ 35 ] PDMS [ [17] , [18] , [19] , 27 , 29 , 30 , [33] , [34] , [35] , [36] , [37] , ...…”

Section: Microfluidic Strategies For the Construction Of Intestinal B...mentioning

confidence: 99%

Establishment and evaluation of on-chip intestinal barrier biosystems based on microfluidic techniques

Wang,

Li,

Shi

et al. 2024

Materials Today Bio

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“…Additionally, PEDOT:PSS offers a favorable compromise between electrical properties and transparency, making it suitable for simultaneous electrical activity recording and optical imaging. A recent study successfully utilized PEDOT:PSS to monitor the impedance of cellular barriers while enabling optical inspection in an organ-on-a-chip [125]. In this work, an organ-on-a-chip device with integrated semitransparent PEDOT:PSS electrodes was developed to monitor intestinal barrier tissue function, overcoming the limitations of current EIS setups in organ-ona-chip systems.…”

Section: Challenges and Potential Solutionsmentioning

confidence: 99%

In situ biosensing technologies for an organ-on-a-chip

Kim,

Jin

et al. 2023

Biofabrication

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The in vitro simulation of organs resolves the accuracy, ethical, and cost challenges accompanying in vivo experiments. Organoids and organs-on-chips have been developed to model the in vitro, real-time biological and physiological features of organs. Numerous studies have deployed these systems to assess the in vitro, real-time responses of an organ to external stimuli. Particularly, organs-on-chips can be most efficiently employed in pharmaceutical drug development to predict the responses of organs before approving such drugs. Furthermore, multi-organ-on-a-chip systems facilitate the close representations of the in vivo environment. In this review, we discuss the biosensing technology that facilitates the in situ, real-time measurements of organ responses as readouts on organ-on-a-chip systems, including multi-organ models. Notably, a human-on-a-chip system integrated with automated multi-sensing will be established by further advancing the development of chips, as well as their assessment techniques.

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Organ-on-a-chip with integrated semitransparent organic electrodes for barrier function monitoring

Abstract: Organs-on-a-chip (OoC) are cell culture platforms that replicate key functional units of tissues in vitro. Barrier integrity and permeability evaluation are of utmost importance when studying barrier-forming tissues. In this...

Cited by 15 publications

References 46 publications

A Conformable Organic Electronic Device for Monitoring Epithelial Integrity at the Air Liquid Interface

A Conformable Organic Electronic Device for Monitoring Epithelial Integrity at the Air Liquid Interface

Establishment and evaluation of on-chip intestinal barrier biosystems based on microfluidic techniques

In situ biosensing technologies for an organ-on-a-chip

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