Organ-On-A-Chip: An Emerging Research Platform

R, Nithin; Aggarwal, Ayushi; Sravani, Anne Boyina; Mallya, Pooja; Lewis, Shaila

doi:10.1080/15476278.2023.2278236

Cited by 6 publications

(1 citation statement)

References 89 publications

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“…Concerning animal models, 3D-cell cultures are still inferior in terms of complexity and the faithful recapitulation of systemic properties, such as drug-induced release of hormones or activation of neural or immune responses. Yet, lab-on-a-chip approaches are increasingly coping even with systemic aspects [4,5], organoids and assembloids are reflecting a growing number of tissue-specific characteristics [6][7][8], and a clear advantage of the cellular models is that these can employ cells of a matched species origin, e.g., human cells for human disease studies, and even using patient-derived cells for personalized questions [7,9].…”

Section: Introductionmentioning

confidence: 99%

A Multiparametric Analysis Reveals Differential Behavior of Spheroid Cultures on Distinct Ultra-Low Attachment Plates Types

Vitacolonna,

Bruch,

Agaçi

et al. 2024

Preprint

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Spheroids have become principal three-dimensional biological models to study cancer, developmental processes, and drug efficacy. For spheroid generation, ultra-low attachment plates are noteworthy due to their simplicity, compatibility with automation, and experimental and commercial accessibility. Nonetheless, it is unknown whether and to what degree the plate type impacts spheroid formation and biology. This study employed automated brightfield microscopy to systematically compare the size and eccentricity of spheroids formed in six different plate types using four distinct human cell lines, i.e., CCD-1137Sk fibroblasts, HaCaT keratinocytes, and MDA-MB-231 and HT-29 cancer cells. Results showed that all plate types exhibited similar spheroid-forming capabilities, and the gross patterns of growth or shrinkage during four days after seeding were comparable. Yet, size and eccentricity varied systematically among specific cell lines and plate types. A confocal wholemount analysis by a novel pipeline of AI-based 3D-image analysis procedures revealed changes in cell proliferation, cell number, nuclear volume, and keratinocyte differentiation, which were accompanied by altered YAP1-signals. The findings show that the plate type may influence the outcome of experimental campaigns. It is advisable to scan different plate types for the optimal configuration for a specific investigation instead of using one standard plate for all kinds of applications.

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Section: Introductionmentioning

confidence: 99%

A Multiparametric Analysis Reveals Differential Behavior of Spheroid Cultures on Distinct Ultra-Low Attachment Plates Types

Vitacolonna,

Bruch,

Agaçi

et al. 2024

Preprint

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Bone and Joint‐on‐Chip Platforms: Construction Strategies and Applications

Du,

Liu,

Liu

et al. 2024

Small Methods

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Organ‐on‐a‐chip, also known as “tissue chip,” is an advanced platform based on microfluidic systems for constructing miniature organ models in vitro. They can replicate the complex physiological and pathological responses of human organs. In recent years, the development of bone and joint‐on‐chip platforms aims to simulate the complex physiological and pathological processes occurring in human bones and joints, including cell–cell interactions, the interplay of various biochemical factors, the effects of mechanical stimuli, and the intricate connections between multiple organs. In the future, bone and joint‐on‐chip platforms will integrate the advantages of multiple disciplines, bringing more possibilities for exploring disease mechanisms, drug screening, and personalized medicine. This review explores the construction and application of Organ‐on‐a‐chip technology in bone and joint disease research, proposes a modular construction concept, and discusses the new opportunities and future challenges in the construction and application of bone and joint‐on‐chip platforms.

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