Organ formation in vitro as applicable to forest tree propagation

Haissig, Bruce E.

doi:10.1007/bf02858610

Cited by 19 publications

(4 citation statements)

References 73 publications

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“…Regeneration of buds and roots in plant tissues is regulated by a number of factors (Leopold 1964, Dore 1965, Haissig 1965). It has long been known that gro-^th regulators play an important role in these processes.…”

Section: Introductionmentioning

confidence: 99%

Regeneration in Streptocarpus Leaf Discs and its Regulation by Temperature and Growth Substances

Appelgren

Heide

1972

Physiologia Plantarum

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The formation of adventitious buds and roots in leaf discs of Streptocarpus x bybridus‘Constant Nymph’ were both stimulated by relatively low temperatures (12 and 18°C) applied to isolated discs or to the growing plants before leaf harvest. Auxins also promoted both bud and root formation, the optimum concentration for rooting always being one to two orders of magnitude higher than the optimum for budding. Cytokinins had only a small stimulatory effect on bud formation. At higher concentrations it was inhibitory and even counteracted the stimulatory effect of auxin on bud formation. As usual, root formation was inhibited by cytokinin. GA3 inhibited both bud and root formation but the inhibition was reversible by auxin. In presence of optimum auxin levels abscisic acid enhanced bud formation. It had little effect on root formation except for an inhibition at high concentrations. The effects of exogenous auxin and cytokinin suggest that Streptocarpus leaves have a high and non‐limiting level of endogenous cytokinin with auxin as the limiting factor for both root and bud formation. This would also explain the exceptionally high regeneration ability of this plant.

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Section: Introductionmentioning

confidence: 99%

Regeneration in Streptocarpus Leaf Discs and its Regulation by Temperature and Growth Substances

Appelgren

Heide

1972

Physiologia Plantarum

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“…Increasing the production of complete plants on the callus would permit the study in vitro of differentiation and morphogenesis in trees, and the required medium might also support the growth of embryoids from isolated single cells or small colonies (Murashige and Nakano, 1966;Tulecke, 1964). On the other hand, our current work indicates that a practical method of clonal propagation (Geissbuhler and Skoog, 1957;Hassig, 1965) might be developed by rooting excised shoots.…”

Section: Methodsmentioning

confidence: 82%

Shoot and Tree Production From Aspen Tissue Cultures

Winton

1970

American J of Botany

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Trees were produced from firm white callus tissue of triploid quaking aspen (Populus tremuloides Michx.), initiated on a modified Wolter and Skoog defined medium and subcultured monthly for two years. When subcultured to medium without auxin, kinetin or supplements, but containing 0.15 mg/liter BAP (6‐benzylaminopurine), multiple stunted shoots appeared on most inocula. However, at 0.05 mg/liter BAP, only a few vigorous shoots per piece were initiated, but seven rooted on the callus: two in the dark with BAP and five in 200 ft‐c of light with 0.04 mg/liter 2,4‐D. After proliferation of the roots on the medium surface, four shoots elongated and were planted in semi‐sterilized soil, then were given 3100 ft‐c of light for rapid growth into trees. Both light sources were on for 16 hr/day. Two trees were also grown from stunted shoots excised from the callus and rooted in soil.

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“…Although ambient temperature is briefly mentioned to modulate the efficiency of in vitro regeneration for several crops, such as tobacco (Nicotiana tabacum), sunflower (Helianthus annuus) or carrot (Daucus carota), in a now quite dated review (Haissig 1965), no molecular mechanisms explaining how temperature affects hormone-induced regeneration have been reported so far. In this study, we show that warm temperature enhances both callus formation and subsequent shoot regeneration in Arabidopsis thaliana, and that the transcriptional events mediating this process correlate with temperature-dependent eviction of H2A.Z.…”

Section: Introductionmentioning

confidence: 99%

Warm Temperature Promotes Shoot Regeneration inArabidopsis thaliana

Lambolez

Kawamura

Takahashi

et al. 2022

Plant and Cell Physiology

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Many plants are able to regenerate upon cutting, and this process can be enhanced in vitro by incubating explants on hormone-supplemented media. While such protocols have been used for decades, little is known about the molecular details of how incubation conditions influence their efficiency. In this study, we find that warm temperature promotes both callus formation and shoot regeneration in Arabidopsis thaliana. We show that such an increase in shoot regenerative capacity at higher temperatures correlates with the enhanced expression of several regeneration-associated genes, such as CUP-SHAPED COTYLEDON 1 (CUC1) encoding a transcription factor involved in shoot meristem formation and YUCCAs (YUCs) encoding auxin biosynthesis enzymes. ChIP-sequencing analyses further reveal that the histone variant H2A.Z is enriched on these loci at 17°C, while its occupancy is reduced by an increase in ambient temperature to 27°C. Moreover, we provide genetic evidence to demonstrate that H2A.Z acts as a repressor of de novo shoot organogenesis, since H2A.Z-depleted mutants display enhanced shoot regeneration. This study thus uncovers a new chromatin-based mechanism that influences hormone-induced regeneration and additionally highlights incubation temperature as a key parameter for optimizing in vitro tissue culture.

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Organ formation in vitro as applicable to forest tree propagation

Cited by 19 publications

References 73 publications

Regeneration in Streptocarpus Leaf Discs and its Regulation by Temperature and Growth Substances

Regeneration in Streptocarpus Leaf Discs and its Regulation by Temperature and Growth Substances

Shoot and Tree Production From Aspen Tissue Cultures

Warm Temperature Promotes Shoot Regeneration inArabidopsis thaliana

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