Organ Culture of Human Somatotrophic Pituitary Adenomas: Ultrastructure and Growth Hormone Production

Peillon, F; Gourmelen, M; Donnadieu, M; Brandi, A. M.; D, Sevaux; Trung, My Tran

doi:10.1530/acta.0.0790217

Cited by 16 publications

(4 citation statements)

References 5 publications

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“…Many investigators have cultured pituitary ade nomas. ', 3,4,8,9,11,12,14,17) We previously reported 6) that monolayer cultures of GH-producing pituitary ade nomas exhibit many degenerative changes in the absence of any drug. Therefore, monolayer cultures are not suitable for investigating morphological changes.…”

Section: Resultsmentioning

confidence: 99%

Bromocriptine-induced Morphological Changes in Cultured Growth Hormone producing Pituitary Adenoma Cells

Inoue

Saito

Kitamura

et al. 1989

Neurol. Med. Chir.(Tokyo)

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Tumor tissues obtained from two patients with growth hormone-producing pituitary adenomas were cultured and treated with bromocriptine, then examined for morphological changes. Untreated tumor cells (controls) were morphologically well preserved and in terms of growth hormone secretion. Tumor cells exposed to bromocriptine for 7 days contained many vacuoles, which, after 14 days of exposure, grew larger and more numerous. These vacuoles appeared to be extensions of endoplasmic reticulum: some were connected to rough or smooth endoplasmic reticulum or, occasionally, to Golgi apparatus, and there were ribosomes on their surfaces. Bromocriptine apparently has cytocidal effects on growth hormone-producing pituitary adenoma in vitro.

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Section: Resultsmentioning

confidence: 99%

Bromocriptine-induced Morphological Changes in Cultured Growth Hormone producing Pituitary Adenoma Cells

Inoue

Saito

Kitamura

et al. 1989

Neurol. Med. Chir.(Tokyo)

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“…However, most other investigators such as Betzdorf et al (1971), Peillon et al (1975) and Kageyama et al (1976) reported the duration of GH secretion to be 2-4 weeks. Minute amounts of GH secretion have been reported by Kageyama et al (1976) in some tumors from nonacromegalic patients, but the amount seemed to be too small to describe to tumoral secretions.…”

Section: Discussionmentioning

confidence: 96%

Growth hormone and prolactin in tissue culture of pituitary adenoma.

et al. 1977

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Pituitary adenomas obtained from 6 patients with acromegaly and 8 patients with chromophobe adenoma were subjected to the tissue culture to correlate in vitro secretion of growth hormone (GH) and prolactin (PRL) to preoperative in vivo endocrine studies and endocrinilogical manifestations. Tissue culture demonstrated that all of the acromegalic tumors secreted large amounts of GH for 2-4 weeks and some of the PRL-secreting chromophobe adenoma without any clinical and endocrinological evidences of GH secretion secreted considerable amounts of GH; however, no recognizable GH secretion was noted in the remaining chromophobe adenomas. PRL was secreted in vitro in large amounts by tumors obtained from patients with hyperprolactinemia with and without acromegaly. It is interesting to note that tumors obtained from 2 patients who showed very slight elevation of plasma PRL of less than 40 ng/ml secreted large amounts of PRL in vitro. This finding suggests that PRL-secreting adenoma could not be ruled out in patients with mild hyperprolactinemia. The duration of in vitro PRL release was longer than that of GH and its rate of decrease was slower than that of GH.

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“…1969;Peillon et al 1975Peillon et al , 1976) the present study was undertaken in order 1) to determine whether in the same culture system PRL secretion and synthesis might occur in these tumours with or without in vivo PRL hypersécrétion 2) to compare the rate and evolution of PRL and GH secretion and synthesis by the same tumours and 3) in addition to evaluate the effect of somatostatin (GHIF) upon PRL secretion from these tumours in short-term incubation.…”

mentioning

confidence: 99%

Prolactin Secretion and Synthesis in Short- And Long-Term Organ Culture of Pituitary Tumours From Acromegalic Patients

Peillon

Cesselin

Garnier³

et al. 1978

Acta Endocrinologica

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To evaluate the in vitro PRL secretion and synthesis by pituitary tumours from acromegalic patients, six somatotrophic and two somatomammotrophic adenomas were collected after surgery and divided into fragments 1 mm3 in size. Fragments were cultured in short-term ( 1\ p=n-\ 2\ p=n-\ 3\ p=n-\ 4 h) and in long-term organ culture (7 and 14 days). Media were collected for GH and PRL radioimmunoassay and fragments studied by electron microscopy. In two experiments, fragments from a somatotrophic adenoma and from a somatomammotrophic adenoma were cultured for 1 to 4 h and 9 to 16 days with [3H]leucine. In addition the effect of somatostatin (2.5 nmole/ml) upon PRL secretion was studied in short-term incubation. In long-term organ culture PRL concentrations increased in 8 out of 16 media samples collected from the five tumours and one normal pituitary cultured for 14 days, while GH concentrations decreased during the same time in all the experiments. The [3H] PRL/[3H] proteins ratios were 9.7 % on the 9th day of culture and 20.8 % on the 16th day while for GH, the ratios were respectively 40 and 34.7 %. Ultrastructural studies showed, besides GH cells, the presence of PRL secreting cells in the different tumours, after 7 or 14 days of culture. In short-term incubation, PRL concentrations in media increased in most of the samples collected from the three tumours incubated for 1 to 4 h and the [3H]PRL/[3H]proteins ratio was 10.6 % after 1 h and 21 % after 2 h of incubation. A decrease of PRL content (< 50 %) was observed in two of the three tumours incubated with somatostatin.These data indicate that 1) somatotrophic adenomas, as well as somatomammotrophic ones, are able to secrete and synthesize PRL in vitro,

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Organ Culture of Human Somatotrophic Pituitary Adenomas: Ultrastructure and Growth Hormone Production

Cited by 16 publications

References 5 publications

Bromocriptine-induced Morphological Changes in Cultured Growth Hormone producing Pituitary Adenoma Cells

Bromocriptine-induced Morphological Changes in Cultured Growth Hormone producing Pituitary Adenoma Cells

Growth hormone and prolactin in tissue culture of pituitary adenoma.

Prolactin Secretion and Synthesis in Short- And Long-Term Organ Culture of Pituitary Tumours From Acromegalic Patients

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