Organ culture of fish tissues

Effect of 17‐α‐ethynylestradiol on germ cell proliferation in organ and primary culture of medaka (Oryzias latipes) testis

“…Short of using complicated perfusion systems, the cultured organs must be either extremely small, or only fragments of organs can be cultured in vitro. The latter approach was taken by Janssens and Grigg (1994), who cultured liver fragments (cubes of approximately 2 mm 3 ) from gold fish (Carassius auratus), carp (Cyprinus carpio) and red-fin perch (Perca fluviatilis) in medium on a slowly rotating roller apparatus. Similarly, single follicles of rainbow trout can be cultured successfully in vitro (Nagler et al 1994).…”

The use of Fish Cells in Ecotoxicology: The Report and Recommendations of ECVAM Workshop 47,

“…Most fish cell work has been driven by commercial aquaculture and fisheries, mainly with the purpose of identifying and growing fish viruses for fish health studies (2,3). However, the scattered literature on fish cells has also touched on various aspects of cellular physiology, molecular biology, genetics, immunology, endocrinology, nutrition, comparative biology and biotechnology (4)(5)(6)(7)(8)(9). One of the first studies that used fish cells in vitro for toxicological studies was that of Rachlin & Perlmutter (10), who measured the cytotoxic action of zinc on an established cell line from the fathead minnow (FHM).…”

Functional identification of an osmotic response element (ORE) in the promoter region of the killifish deiodinase 2 gene (FhDio2)

“…The L-15 medium in the experimental plates was made hypotonic by simple dilution 1:1, restoring its nutrients with an amino acid stock solution (Sigma-Aldrich, St Louis, MO, USA) and galactose 10·g·l -1 . The explants were incubated for 6·h in a metabolic bath chamber at 28°C under continuous agitation and saturating humidity with 99% O 2 and 1% CO 2 (Janssens and Grigg, 1994). Explants and the corresponding culture media were separately collected after 2, 4 and 6·h of incubation.…”