Orengedokuto exerts anti-allergic effects via inhibition of effector T cell activation in a murine model of contact hypersensitivity

Tsuge, Atsushi; Watanabe, Atsuki; Kodama, Yuki; Hisaka, Shinsuke; Nose, Mitsuhiko

doi:10.1007/s11418-021-01566-2

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…In addition, skin inflammation and itch-related behavior in animal models of atopic dermatitis were also improved by repetitive oral administration of Orengedokuto [7] and berberine [8]. Orengedokuto also attenuates the inflammatory symptoms in mite-induced allergic dermatitis [9] and chemical allergen-induced contact dermatitis [10,11]. In addition to our report [8], berberine also exerts an anti-inflammatory effect in 12-O-tetradecanoylphorbol-13-acetate-induced dermatitis [12], allergic contact dermatitis [13,14] and fungus-induced dermatitis [5] in animals.…”

Section: Tsugunobu Andoh and Tadamichi Shimizumentioning

confidence: 99%

The Molecular Mechanisms underlying the Therapeutic Effect of Berberine in Inflammatory Skin Diseases

Andoh¹,

Shimizu²

2023

Asian J Complement Altern Med

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Clinical therapy of chronic inflammatory skin diseases such as atopic dermatitis is extremely difficult. Recently, the use of a number of traditional herbal medicines has been attempted to treat these skin diseases. Orengedokuto is a traditional herbal medicine consisting of four crude natural medicines, and in the field of dermatology, it is used to treat skin diseases accompanied by inflammation and pruritus. Our recent animal study showed that berberine, a major component of Orengedokuto, improves skin inflammation and itching in mice with atopy-like dermatitis. Furthermore, we identified EIF3F and MALT1 as factors involved in the suppression of cytokine production by berberine. In addition, berberine also activates AMP-activated protein kinase, which is involved in the expression of inflammatory and anti-inflammatory cytokines. In addition, berberine also attenuates epidermal hyperplasia though the inhibition of CDC6 expression. Taken together, these findings indicate that berberine improves inflammatory skin diseases by controlling gene expression related to both inflammation, anti-inflammation and keratinocyte hyperproliferation.

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Section: Tsugunobu Andoh and Tadamichi Shimizumentioning

confidence: 99%

The Molecular Mechanisms underlying the Therapeutic Effect of Berberine in Inflammatory Skin Diseases

Andoh¹,

Shimizu²

2023

Asian J Complement Altern Med

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“…Kampo medicines are gaining attention as an alternative or complementary treatment option to Western medicines, particularly for chronic conditions that may not be fully addressed using conventional medicine alone. In recent years, there has been increasing research on Kampo medicines, with studies focusing on their efficacy, safety, and mechanism of action [15][16][17]. As part of our ongoing research to scientifically clarify the effectiveness of Kampo medicines [18][19][20][21], this study evaluated the anti-glycation activity of a total of 147 prescriptions of oral Kampo medicines covered by health insurance in Japan.…”

Section: Introductionmentioning

confidence: 99%

“…Figure 8. MS/MS fragmentation pathway of compounds 12 and 16.Peaks 5 (tR 5.76 min), 8 (tR 6.57 min), 15 (tR 10.38 min), 22 (tR 14.86 min), and 23 (tR 15.20 min) were identified as characteristic monoterpenoids in Paeoniae radix[39].Peaks 5, 15, and 8 were identified as albiflorin (5), 4-epi-albiflorin(15), and peoniflorin(8), with the same molecular formula (C23H28O11 each), and they were deduced from the ions of m/z 498.1966 [M+NH4] + , 481.1699 [M+H] + , and m/z 481.1704 [M+H] + , respectively. Fragmentation of 5 and 15 generated ions 5a and 15a via the loss of a glucose, followed by the loss of H2O and the benzoyl group to generate product ions 5b and 15b (Figure9).…”

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confidence: 99%

LC-MS Profiling of Kakkonto and Identification of Ephedrine as a Key Component for Its Anti-Glycation Activity

Ito,

Kikuchi,

Ikube

et al. 2023

Molecules

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A total of 147 oral Kampo prescriptions, which are used clinically in Japan, were evaluated for their anti-glycation activity. Kakkonto demonstrated significant anti-glycation activity, prompting further analysis of its chemical constituents using LC-MS, which revealed the presence of two alkaloids, fourteen flavonoids, two but-2-enolides, five monoterpenoids, and four triterpenoid glycosides. To identify the components responsible for its anti-glycation activity, the Kakkonto extract was reacted with glyceraldehyde (GA) or methylglyoxal (MGO) and analyzed using LC-MS. In LC-MS analysis of Kakkonto reacted with GA, the peak intensity of ephedrine was attenuated, and three products from ephedrine-scavenging GA were detected. Similarly, LC-MS analysis of Kakkonto reacted with MGO revealed two products from ephedrine reacting with MGO. These results indicated that ephedrine was responsible for the observed anti-glycation activity of Kakkonto. Ephedrae herba extract, which contains ephedrine, also showed strong anti-glycation activity, further supporting ephedrine’s contribution to Kakkonto’s reactive carbonyl species’ scavenging ability and anti-glycation activity.

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Hochuekkito exerts the anti-allergic effects via activating regulatory T cells in a murine model of contact hypersensitivity

et al. 2023

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Orengedokuto exerts anti-allergic effects via inhibition of effector T cell activation in a murine model of contact hypersensitivity

Cited by 3 publications

References 27 publications

The Molecular Mechanisms underlying the Therapeutic Effect of Berberine in Inflammatory Skin Diseases

The Molecular Mechanisms underlying the Therapeutic Effect of Berberine in Inflammatory Skin Diseases

LC-MS Profiling of Kakkonto and Identification of Ephedrine as a Key Component for Its Anti-Glycation Activity

Hochuekkito exerts the anti-allergic effects via activating regulatory T cells in a murine model of contact hypersensitivity

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