OregonFluor enables quantitative intracellular paired agent imaging to assess drug target availability in live cells and tissues

Label-free analysis of membrane protein binding kinetics and cell adhesions using evanescent scattering microscopy

“…By fitting the binding curve with a first-order binding kinetics model, the k on , k off , and K D were found to be 8.0 × 10 4 M −1 s −1 , 4.1 × 10 −3 s −1 , and 51.3 nM, which are close to the reported values, showing that the spring constant measurements can resist the interference resulting from large cell micromotions and determine the kinetics of small molecule binding to membrane proteins. 37…”

Small-molecule probes from bench to bedside: advancing molecular analysis of drug–target interactions toward precision medicine

“…, probe design and technology development) will bring unprecedented opportunities to facilitate drug discovery and personalized medicine. 306,307 For example, new probe and assay designs with spatially precise photo-proximity labeling 308 and dynamic ratiometric imaging 309 are likely to further enhance the specificity, sensitivity, and accuracy of small-molecule probes to generate highly reliable data for drug evaluation, against complex background of different biological specimens. Likewise, technology advances in multiplexed microfluidic systems 310 and cycling imaging 311 which enhance information acquisition, at a high molecular resolution and processing throughput, can reveal previously masked signatures, generate multiparametric and multipopulation analysis, and establish disease-reflective biomarker composites.…”

Strategies to convert organic fluorophores into red/near-infrared emitting analogues and their utilization in bioimaging probes

“…The C-9 central atom of the xanthene dyes can be chosen from the carbon family (group 14: C, Si, Ge, and Sn), leading to various xanthene analogues, 11–23 as well as from the nitrogen family (group 15; N and P) 16,24 or chalcogen family (group 16; O, S, Se, and Te) 16,25–28 (Fig. 4).…”