Orderly Arranged Fluorescence Dyes as a Highly Efficient Chemiluminescence Resonance Energy Transfer Probe for Peroxynitrite

Abstract

Chemiluminescence (CL) probes for reactive oxygen species (ROS) are commonly based on a redox reaction between a CL reagent and ROS, leading to poor selectivity toward a specific ROS. The energy-matching rules in the chemiluminescence resonance energy transfer (CRET) process between a specific ROS donor and a suitable fluorescence dye acceptor is a promising method for the selective detection of ROS. Nevertheless, higher concentrations of fluorescence dyes can lead to the intractable aggregation-caused quenching effect, decreasing the CRET efficiency. In this report, we fabricated an orderly arranged structure of calcein-sodium dodecyl sulfate (SDS) molecules to improve the CRET efficiency between ONOOH* donor and calcein acceptor. Such orderly arranged calcein-SDS composites can distinguish peroxynitrite (ONOO(-)) from a variety of other ROS owing to the energy matching in the CRET process between ONOOH* donor and calcein acceptor. Under the optimal experimental conditions, ONOO(-) could be assayed in the range of 1.0-20.0 μM, and the detection limit for ONOO(-) [signal-to-noise ratio (S/N) = 3] was 0.3 μM. The proposed strategy has been successfully applied in both detecting ONOO(-) in cancer mouse plasma samples and monitoring the generation of ONOO(-) from 3-morpholinosydnonimine (SIN-1). Recoveries from cancer mouse plasma samples were in the range of 96-105%. The success of this work provides a unique opportunity to develop a CL tool to monitor ONOO(-) with high selectivity in a specific manner. Improvement of selectivity and sensitivity of CL probes holds great promise as a strategy for developing a wide range of probes for various ROS by tuning the types of fluorescence dyes.

A dual-readout chemiluminescent-gold lateral flow test for multiplex and ultrasensitive detection of disease biomarkers in real samples

“…All the negative samples (1-10) are detected to be negative using C-mode GLFT, CMIA and ELISA. For positive samples (11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30), sample 26 is detected to be negative by these three methods, and sample 27 is also detected to be negative using ELISA ( Figure S11). However, the sample 27 is correctly detected to be positive using C-mode GLFT and CMIA ( Figure 6).…”

Gold Nanoclusters@Ru(bpy)32+-Layered Double Hydroxide Ultrathin Film as a Cathodic Electrochemiluminescence Resonance Energy Transfer Probe

“…As a result, the fluorescence lifetime and quantum yield of fluorescence dyes would increase when they were assembled on the surface of LDHs. 32,33 The successful improvements of FRET and CRET in the LDH matrix inspirits us to construct an efficient ERET process by layer-by-layer (LBL) electrostatic assembly of CoAl−LDH nanosheets with some ECL molecules (i.e., ERET donors and acceptors). However, the ERET with LDH nanosheets have not been established yet.…”

Donor/acceptor nanoparticle pair-based singlet oxygen channeling homogenous chemiluminescence immunoassay for quantitative determination of bisphenol A

“…Chemiluminescence (CL) has become very popular as an analytical technique in recent years, as they offer significant gains in sensitivity without interference from background light and rapidity at a low cost, both in instrumentation and materials [30][31][32][33]. While, to the best of our knowledge, there was no report about chemiluminescence homogenous immunoassay for the detection of BPA, in this study, we developed a singlet oxygen channeling chemiluminescence homogenous immunoassay for the quantitative determination of BPA in environmental water samples.…”